Biofilm composition and composite degradation during intra-oral wear

Yulianto, Heribertus Dedy Kusuma; Rinastiti, Margareta; Cune, Marco S.; Haan-Visser, Willy de; Atema-Smit, Jelly; Busscher, Henk J.; Mei, Henny C. van der

doi:10.1016/j.dental.2019.02.024

Cited by 48 publications

(33 citation statements)

References 44 publications

(64 reference statements)

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“…More recent studies have shown the important role of bacteria and esterase commonly found in the oral environment in the degradation of resin composites. The ester-linkages in Bis-GMA and TEGDMA composites are subjected to hydrolysis when exposed to enzymes and an esterase, produced by Streptococcus mutans , seems to be partly responsible for this intraoral degradation [32]. After exposure to esterase enzyme, a nanofilled composite, Filtek supreme plus, showed 57% reduction in the tensile diametral strength and 46% in elasticity [33].…”

Section: Discussionmentioning

confidence: 99%

Influences of Different Air-Inhibition Coatings on Monomer Release, Microhardness, and Color Stability of Two Composite Materials

Marigo

Nocca

Fiorenzano

et al. 2019

BioMed Research International

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The aim of this study was to evaluate the effect of light-curing protocols on two modern resin composites using different air-inhibition coating strategies. This was accomplished by assessing the amount of monomer elution, surface microhardness, and composite discoloration in different storage conditions. A total of 120 specimens were prepared using Filtek Supreme XTE (3M ESPE, Seefeld, Germany) and CeramX Universal (Dentsply DeTrey, Konstanz, Germany). Specimens were light-cured in air as per manufacturer’s instructions or in the absence of oxygen. This latter condition was achieved using three different approaches: (i) transparent polyester strip; (ii) glycerin; (iii) argon gas. Specimens were assessed for release of monomers, Vickers hardness, and discoloration after storage in different solutions. The results were analyzed with ANOVA one-way test followed by Student-Newman-Keuls test. Moreover, multiple comparisons of means were performed using the Student t-test (p<0.05). The amount of monomers released from the tested specimens was very low in all conditions. The presence of oxygen induced some decrease in microhardness. The highest discoloration values, for both materials, were obtained after ageing in red wine. In case finish and polish procedures are awkward to achieve in posteriors composite restoration, light-curing in the absence of oxygen should be considered, especially when performing composite restoration in esthetic areas.

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Section: Discussionmentioning

confidence: 99%

Influences of Different Air-Inhibition Coatings on Monomer Release, Microhardness, and Color Stability of Two Composite Materials

Marigo

Nocca

Fiorenzano

et al. 2019

BioMed Research International

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“…This bacterium is Gram-positive, and it is present in intraoral multispecies biofilms [43]. S. mutans group is the main bacteria associated with caries lesion development [44], and they can attach to dental and material surfaces [1,21]. The samples were exposed to planktonic as well as biofilm stages.…”

Section: Discussionmentioning

confidence: 99%

“…The oral environment provides many challenges to the physical and chemical stability of dental resins, such as high humidity, temperature, and pH variations [19,20]. Likewise, acids leached by high acidogenic caries-linked bacteria such as Streptococcus mutans, conjointly with a degradative attack of enzymes, inherently present in the saliva, can jeopardize the materials' properties over time [21]. More expressively, acidic attack from high acidogenic caries-linked bacteria is a crucial step in tooth demineralization around the restorations [22].…”

Section: Introductionmentioning

confidence: 99%

Myristyltrimethylammonium Bromide (MYTAB) as a Cationic Surface Agent to Inhibit Streptococcus mutans Grown over Dental Resins: An In Vitro Study

Nunes

et al. 2020

JFB

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This in vitro study evaluated the effect of myristyltrimethylammonium bromide (MYTAB) on the physical, chemical, and biological properties of an experimental dental resin. The resin was formulated with dental dimetacrylate monomers and a photoinitiator/co-initiator system. MYTAB was added at 0.5 (G 0.5% ), 1 (G 1% ), and 2 (G 2% ) wt %, and one group remained without MYTAB and was used as the control (G Ctrl ). The resins were analyzed for the polymerization kinetics, degree of conversion, ultimate tensile strength (UTS), antibacterial activity against Streptococcus mutans, and cytotoxicity against human keratinocytes. Changes in the polymerization kinetics profiling were observed, and the degree of conversion ranged from 57.36% (±2.50%) for G 2% to 61.88% (±1.91%) for G 0.5% , without a statistically significant difference among groups (p > 0.05). The UTS values ranged from 32.85 (±6.08) MPa for G 0.5% to 35.12 (±5.74) MPa for G Ctrl (p > 0.05). MYTAB groups showed antibacterial activity against biofilm formation from 0.5 wt % (p < 0.05) and against planktonic bacteria from 1 wt % (p < 0.05). The higher the MYTAB concentration, the higher the cytotoxic effect, without differences between G Ctrl e G 0.5% (p > 0.05). In conclusion, the addition of 0.5 wt % of MYTAB did not alter the physical and chemical properties of the dental resin and provided antibacterial activity without cytotoxic effect.

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“…Although the available data suggest that microbial succession on dental materials is similar to that on sound enamel, differences in the abundance of relevant species may still exist. This was demonstrated in an in vivo study comparing oral biofilm formation on dental composites containing higher versus lower ester-linkages, with a greater abundance of Streptococcus mutans on the former (Kusuma Yulianto et al 2019). Certainly, further research is needed to better characterize biofilm formation, microbial succession, and species abundance on dental materials.…”

Section: Microbe-acquired Pellicle Interactions During Dental Biofilm Formationmentioning

confidence: 95%

Interaction between the Oral Microbiome and Dental Composite Biomaterials: Where We Are and Where We Should Go

et al. 2020

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Dental composites are routinely placed as part of tooth restoration procedures. The integrity of the restoration is constantly challenged by the metabolic activities of the oral microbiome. This activity directly contributes to a less-than-desirable half-life for the dental composite formulations currently in use. Therefore, many new antimicrobial dental composites are being developed to counteract the microbial challenge. To ensure that these materials will resist microbiome-derived degradation, the model systems used for testing antimicrobial activities should be relevant to the in vivo environment. Here, we summarize the key steps in oral microbial colonization that should be considered in clinically relevant model systems. Oral microbial colonization is a clearly defined developmental process that starts with the formation of the acquired salivary pellicle on the tooth surface, a conditioned film that provides the critical attachment sites for the initial colonizers. Further development includes the integration of additional species and the formation of a diverse, polymicrobial mature biofilm. Biofilm development is discussed in the context of dental composites, and recent research is highlighted regarding the effect of antimicrobial composites on the composition of the oral microbiome. Future challenges are addressed, including the potential of antimicrobial resistance development and how this could be counteracted by detailed studies of microbiome composition and gene expression on dental composites. Ultimately, progress in this area will require interdisciplinary approaches to effectively mitigate the inevitable challenges that arise as new experimental bioactive composites are evaluated for potential clinical efficacy. Success in this area could have the added benefit of inspiring other fields in medically relevant materials research, since microbial colonization of medical implants and devices is a ubiquitous problem in the field.

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Biofilm composition and composite degradation during intra-oral wear

Cited by 48 publications

References 44 publications

Influences of Different Air-Inhibition Coatings on Monomer Release, Microhardness, and Color Stability of Two Composite Materials

Influences of Different Air-Inhibition Coatings on Monomer Release, Microhardness, and Color Stability of Two Composite Materials

Myristyltrimethylammonium Bromide (MYTAB) as a Cationic Surface Agent to Inhibit Streptococcus mutans Grown over Dental Resins: An In Vitro Study

Interaction between the Oral Microbiome and Dental Composite Biomaterials: Where We Are and Where We Should Go

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