Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae

Matsushika, Akinori; Inoue, Hiroyuki; Murakami, Katsuji; Takimura, Osamu; Sawayama, Shigeki

doi:10.1016/j.biortech.2008.11.047

Cited by 117 publications

(89 citation statements)

References 28 publications

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“…The microorganisms commonly used for industrial alcohol production have several advantages. However, they exhibit some problems to assimilate pentoses carbohydrates [3]. Moreover, it has been reported that microorganisms that assimilate pentoses show low tolerance to inhibitors and require a small and well-controlled supply of oxygen to enhance alcohol production [3,4], therefore research of wild microorganisms adapted to specific substrates is still considered and attractive alternative to pentoses fermentation, since the knowledge of the strains behavior could be useful for future strategies and for its improvement.…”

Section: Introductionmentioning

confidence: 99%

Capability of Candidas to Ferment Mixtures of Carbohydrates to Alcohol in Free Cells and Co-Culture

Estrada-Martínez¹,

Pacheco-López²,

Ramírez‐Sucre³

et al. 2017

JFSE

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Abstract:Organic biomass is an attractive feedstock for second generation alcohol production. Wild-type strains of the genus Candida showed capabilities different to produce alcohol fermenting a carbohydrates mixture (synthetic medium), individually and in co-culture. Therefore, the main objective of this work was to evaluate the capability of Candida wild-type strains isolated from termite gut and rumen liquid, to ferment the most commonly carbohydrates presented in citrus residues, individually and in co-culture to alcohol production. C. Tropicalis (LR4) presented higher percentage of carbohydrate consumption (74.20% ± 4.60%), alcohol production (44.53 ± 0.01 gL -1 ) and maximal alcohol productivity (6.40 ± 0.01 gL -1 day) than C. Glabrata (T1). Co-culture schemas, CC1 (LR4: 60%; T1: 40%) and CC3 (first LR4 alone and 2 days later T1) presented the highest alcohol production (45.20 ± 1.30 gL -1 and 46.80 ± 2.60 gL -1 , respectively). Maximal alcohol productivity was obtained with CC2 (LR4: 80%; T1: 20%) and CC3 schemas, 7.70 ± 0.29 gL -1 day and 7.80 ± 0.44 gL -1 day, respectively. The results suggest the usefulness of these wild-type strains in co-culture as an alternative to alcohol production from carbohydrates mixtures at concentrations commonly found in citrus waste.

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Section: Introductionmentioning

confidence: 99%

Capability of Candidas to Ferment Mixtures of Carbohydrates to Alcohol in Free Cells and Co-Culture

Estrada-Martínez¹,

Pacheco-López²,

Ramírez‐Sucre³

et al. 2017

JFSE

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“…They are characterized by the ability to ferment xylose to ethanol in synthetic medium. [12][13][14][15][16][17] Among these strains, MA-R4, engineered by chromosomal integration from industrial diploid S. cerevisiae strain IR-2 to express the XYL1, XYL2, and XKS1 genes, converted xylose to ethanol faster than recombinant haploid laboratory strains.…”

Section: )mentioning

confidence: 99%

“…18) Hence the choice of a potential host for developing recombinant strains is important. We have investigated ethanol production from eucalyptus hydrolysate by three recombinant industrial strains, MA-R4, MA-S4, and MA-T4, 15) with the result that MA-R4 rapidly converted xylose to ethanol.…”

Section: )mentioning

confidence: 99%

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Comparison of the Performance of Eight Recombinant Strains of Xylose-FermentingSaccharomyces cerevisiaeas to Bioethanol Production from Rice Straw Enzymatic Hydrolyzate

Fujii

Matsushika

Goshima

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…The utilization of hexose and pentose sugars has been developed through several well-defined strategies, including cell surface display and cellobiose/xylose co-fermentation [8,9]. In addition to laboratorial S. cerevisiae strains, an industrial S. cerevisiae strain has been engineered for cellulosic biofuel production [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

The Application of Thermotolerant Yeast Kluyveromyces marxianus as a Potential Industrial Workhorse for Biofuel Production

Park¹,

Kim²,

Jang³

et al. 2015

KSBB Journal

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Kluyveromyces marxianus is a well-known thermotolerant yeast. Although Saccharomyces cerevisiae is the most commonly used yeast species for ethanol production, the thermotolerant K. marxianus is more suitable for simultaneous saccharification and fermentation (SSF) processes. This is because enzymatic saccharification usually requires a higher temperature than that needed for the optimum growth of S. cerevisiae. In this study, we compared the fermentation patterns of S. cerevisiae and K. marxianus under various temperatures of fermentation. The results show that at a fermentation temperature of 45°C, K. marxianus exhibited more than two fold higher growth rate and ethanol production rate in comparison to S. cerevisiae. For SSF using starch or corn stover as the sole carbon source by K. marxianus, the high temperature (45°C) fermentations showed higher enzymatic activities and ethanol production compared to SSF at 30°C. These results demonstrate the potential of the thermotolerant yeast K. marxianus for SSF in the industrial production of biofuels.

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Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae

Cited by 117 publications

References 28 publications

Capability of Candidas to Ferment Mixtures of Carbohydrates to Alcohol in Free Cells and Co-Culture

Capability of Candidas to Ferment Mixtures of Carbohydrates to Alcohol in Free Cells and Co-Culture

Comparison of the Performance of Eight Recombinant Strains of Xylose-FermentingSaccharomyces cerevisiaeas to Bioethanol Production from Rice Straw Enzymatic Hydrolyzate

The Application of Thermotolerant Yeast Kluyveromyces marxianus as a Potential Industrial Workhorse for Biofuel Production

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