The Application of Thermotolerant Yeast Kluyveromyces marxianus as a Potential Industrial Workhorse for Biofuel Production

Park, Jae-Bum; Kim, Jin-Seong; Jang, Seung-Won; Hong, Eunsoo; Ha, Suk‐Jin

doi:10.7841/ksbbj.2015.30.3.125

Cited by 7 publications

(3 citation statements)

References 25 publications

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“…In these processes, thermotolerant yeasts are necessary, because saccharification, generally considered as the overall rate-determining step in SSF or SSCF, is performed by cellulase enzymes with high optimal reaction temperatures (45–55 °C) [14, 17]. A well known thermotolerant yeast, K. marxianus , is a robust strain used for industrial ethanol production [18] and was previously reported to grow and ferment in environments up to 50 °C [19]. K. marxianus also has various benefits over mesophilic yeasts, such as high growth rates, reduced cooling costs, and a broad spectrum of substrates, including xylose [20, 21].…”

Section: Introductionmentioning

confidence: 99%

Alleviation of catabolite repression in Kluyveromyces marxianus: the thermotolerant SBK1 mutant simultaneously coferments glucose and xylose

Kim

Kwon

Park

et al. 2019

Biotechnol Biofuels

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Background Simultaneous cofermentation of glucose and xylose mixtures would be a cost-effective solution for the conversion of cellulosic biomass to high-value products. However, most yeasts ferment glucose and xylose sequentially due to glucose catabolite repression. A well known thermotolerant yeast, Kluyveromyces marxianus , was selected for this work because it possesses cost-effective advantages over Saccharomyces cerevisiae for biofuel production from cellulosic biomass. Results In the present study, we employed a directed evolutionary approach using 2-deoxyglucose to develop a thermotolerant mutant capable of simultaneous cofermentation of glucose and xylose by alleviating catabolite repression. The selected mutant, K. marxianus SBK1, simultaneously cofermented 40 g/L glucose and 28 g/L xylose to produce 23.82 g/L ethanol at 40 °C. This outcome corresponded to a yield of 0.35 g/g and productivity of 0.33 g/L h, representing an 84% and 129% improvement, respectively, over the parental strain. Interestingly, following mutagenesis the overall transcriptome of the glycolysis pathway was highly downregulated in K. marxianus SBK1, except for glucokinase-1 (GLK1) which was 21-fold upregulated. Amino acid sequence of GLK1 from K. marxianus SBK1 revealed three amino acid mutations which led to more than 22-fold lower enzymatic activity compared to the parental strain. Conclusions We herein successfully demonstrated that the cofermentation of a sugar mixture is a promising strategy for the efficient utilization of cellulosic biomass by K. marxianus SBK1. Through introduction of additional biosynthetic pathways, K. marxianus SBK1 could become a chassis-type strain for the production of fuels and chemicals from cellulosic biomass. Electronic supplementary material The online version of this article (10.1186/s13068-019-1431-x) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Alleviation of catabolite repression in Kluyveromyces marxianus: the thermotolerant SBK1 mutant simultaneously coferments glucose and xylose

Kim

Kwon

Park

et al. 2019

Biotechnol Biofuels

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“…To enhance the feasibility of conventional process and develop SSF and CBP using lignocellulosic biomass, robust yeast strains with multiple tolerance against high temperature, acidic condition and furan derivatives should be developed. By a series of screening methods, metabolic engineering and evolutionary engineering, mutant or recombinant strains of conventional ( Saccharomyces cerevisiae ), and non‐conventional yeasts ( Zygosaccharomyces bailii, Kluyveromyces marxianus and Hansenula polymorpha ) have been developed to be able to grow over 40–45 °C or in the presence of 24 g L −1 weak acid . In additions, recombinant S. cerevisiae strains overexpressing the HAA1 gene or deficient in the OPI1 gene possessed higher tolerance on acetic acid or lactic acid, respectively.…”

Section: Introductionmentioning

confidence: 99%

Physiological and Metabolomic Analysis of Issatchenkia orientalis MTY1 With Multiple Tolerance for Cellulosic Bioethanol Production

Seong

Lee

et al. 2017

Biotechnology Journal

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Yeast with multiple tolerance onto harsh conditions has a number of advantages for bioethanol production. In this study, an alcohol yeast of Issatchenkia orientalis MTY1 is isolated in a Korean winery and its multiple tolerance against high temperature and acidic conditions is characterized in microaerobic batch cultures and by metabolomic analysis. In a series of batch cultures using 100 g L glucose, I. orientalis MTY1 possesses wider growth ranges at pH 2-8 and 30-45 °C than a conventional yeast of Saccharomyces cerevisiae D452-2. Moreover, I. orientalis MTY1 showes higher cell growth and ethanol productivity in the presence of acetic acid or furfural than S. cerevisiae D452-2. I. orientalis MTY1 produces 41.4 g L ethanol with 1.5 g L h productivity at 42 °C and pH 4.2 in the presence of 4 g L acetic acid, whereas a thermo-tolerant yeast of Kluyvermyces marxianus ATCC36907 does not grow. By metabolomics by GC-TOF MS and statistical analysis of 125 metabolite peaks, it is revealed that the thermo-tolerance of I. orientalis MTY1 might be ascribed to higher contents of unsaturated fatty acids, purines and pyrimidines than S. cerevisiae D452-2. Conclusively, I. orientalis MTY1 could be a potent workhorse with multiple tolerance against harsh conditions considered in cellulosic bioethanol production.

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“…Therefore, it is necessary to be able to shorten these processes, and the employed strain should be capable of using various substrates. The strain Kluyveromyces marxianus is currently known to exhibit thermotolerance, making it an industrially robust yeast, because economical production processes, such as simultaneous saccharification and fermentation (SSF) or simultaneous saccharification and co-fermentation (SSCF), prefer high temperatures [5][6][7]. Above all, K. marxianus can utilize various substrates including xylose [6,[8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Mutant Galactose Permease (ScGal2_N376F) Effective for Utilization of Glucose/Xylose or Glucose/ Galactose Mixture by Engineered Kluyveromyces marxianus

Kwon

Kim

Park

et al. 2020

J. Microbiol. Biotechnol.

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Mutant sugar transporter ScGAL2-N376F was overexpressed in Kluyveromyces marxianus for efficient utilization of xylose, which is one of the main components of cellulosic biomass. K. marxianus ScGal2_N376F, the ScGAL2-N376F-overexpressing strain, exhibited 47.04 g/l of xylose consumption and 26.55 g/l of xylitol production, as compared to the parental strain (24.68 g/l and 7.03 g/l, respectively) when xylose was used as the sole carbon source. When a mixture of glucose and xylose was used as the carbon source, xylose consumption and xylitol production rates were improved by 195% and 360%, respectively, by K. marxianus ScGal2_N376F. Moreover, the glucose consumption rate was improved by 27% as compared to that in the parental strain. Overexpression of both wild-type ScGAL2 and mutant ScGAL2-N376F showed 48% and 52% enhanced sugar consumption and ethanol production rates, respectively, when a mixture of glucose and galactose was used as the carbon source, which is the main component of marine biomass. As shown in this study, ScGAL2-N376F overexpression can be applied for the efficient production of biofuels or biochemicals from cellulosic or marine biomass.

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The Application of Thermotolerant Yeast Kluyveromyces marxianus as a Potential Industrial Workhorse for Biofuel Production

Cited by 7 publications

References 25 publications

Alleviation of catabolite repression in Kluyveromyces marxianus: the thermotolerant SBK1 mutant simultaneously coferments glucose and xylose

Alleviation of catabolite repression in Kluyveromyces marxianus: the thermotolerant SBK1 mutant simultaneously coferments glucose and xylose

Physiological and Metabolomic Analysis of Issatchenkia orientalis MTY1 With Multiple Tolerance for Cellulosic Bioethanol Production

Overexpression of Mutant Galactose Permease (ScGal2_N376F) Effective for Utilization of Glucose/Xylose or Glucose/ Galactose Mixture by Engineered Kluyveromyces marxianus

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