Bioengineering Approaches to Controlled Protein Delivery

Abstract

Proteins are of crucial importance in all biologic organisms, in terms of both structure and function. Their deficits play central roles in many pathologic states, and their potential as powerful therapeutic agents has been widely recognized. Many issues, however, exist in delivery of biologically active proteins to target tissues and organs. Recent advances in biomedical engineering have lead to development of advanced techniques for controlled delivery of peptides and proteins, paving the way for their efficient use in treating human injury and disease. With a particular emphasis on most recent advances, this review discusses currently available techniques for controlled delivery of proteins and considers future research directions.

Incorporation of PLGA nanoparticles into porous chitosan–gelatin scaffolds: Influence on the physical properties and cell behavior

“…However, systemic administration of biomolecules such as growth factors often produces poor results, probably due to their short biological half life, lack of tissue specificity, long term instability, and potential dose dependent carcinogenicity (Kobsa and Saltzman, 2008;Lee and Shin, 2007). In addition to this, a well-timed and localized delivery of biomolecules from the scaffold is necessary to achieve the desired biomimetic effect (Vasita and Katti, 2006;Zisch et al, 2003).…”

“…Such a system primarily acts as a local regulator to control doses and kinetics of released growth factor, thus increasing their potential retention time at therapeutic concentration levels (Kobsa andSaltzman, 2008 andSilvia et al, 2007).…”

Enzymosomes: A Rising Effectual Tool for Targeted Drug Delivery System

“…Regardless of the practical advantages of the enzyme transduction technology, the main interest of this plan is the inefficient escape from the endosome to cytosol thus leading to CPP-tagged cargoes secluded in intracellular vesicles. Proteins are of importance to biological needs and thus for controlling delivery of these peptides, modern techniques of encapsulation paved way for its adorable use in the treatment of various diseases [49].…”

Tissue Engineering and Regenerative Science in Pediatrics

“…When a bioactive material is used as the scaffold, the biomaterial may provide the necessary cues to the seeded cells to guide regeneration. External forces may also be important in influencing cell behavior, especially for cells that normally respond to shear and stress, such as endothelial cells in blood vessels and chondrocytes in cartilage (19). When the dominant signaling cue is a single molecule such as the basic fibroblast growth factor, the delivery of such proteins will enhance tissue regeneration (20).…”