Bioengineered acellular dermal matrices for the repair of abdominal wall defects in rats

Mohsina, A; Kumar, Naveen; Sharma, Ashok; Mishra, Birendra Kumar; Mathew, Dayamon David; Remya, V.; Negi, Mamta; Kritaniya, Deepika; Mahan, Patrick; Maiti, S. K.; Shrivastava, Sameer; Singh, Kiranjeet

doi:10.1007/s10029-014-1308-7

Cited by 11 publications

(6 citation statements)

References 20 publications

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“…A large range of scaffolds, including ECM materials, are commercially available for internal tissue engineering and for dermal reconstruction. − It is vital that the physical properties of the material selected for use render it able to withstand the strains to which it will be subject in situ. Common clinical applications include: abdominal hernia repair, providing support during breast reconstruction, rotator cuff repair, tendon repair, and substituting for skin in serious burns and chronic wounds …”

Section: Introductionmentioning

confidence: 99%

“…2−4 It is vital that the physical properties of the material selected for use render it able to withstand the strains to which it will be subject in situ. Common clinical applications include: abdominal hernia repair, 5 providing support during breast reconstruction, 6 rotator cuff repair, 7 tendon repair, 8 and substituting for skin in serious burns and chronic wounds. 9 Surgeons can choose to use either synthetic or natural scaffolds.…”

Section: Introductionmentioning

confidence: 99%

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Collagen Fibril Response to Strain in Scaffolds from Ovine Forestomach for Tissue Engineering

Sizeland

Wells

Kelly

et al. 2017

ACS Biomater. Sci. Eng.

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Scaffold biomaterials are typically applied surgically as reinforcement for weakened or damaged tissue, acting as substrates on which healing tissue can grow. Natural extracellular matrix (ECM) materials consisting mainly of collagen are often used for this purpose, but are anisotropic. Ovine forestomach matrix (OFM) ECM was exposed to increasing strain and synchrotron-based SAXS diffraction patterns and revealed that the collagen fibrils within underwent changes in orientation, orientation index (a measure of isotropy), and extension. Response to the strain depended on the direction the collagen fibrils were oriented. When the ECM was stretched in the direction of collagen fibril orientation, the fibrils become more oriented and begin to take up the strain immediately (as shown by the increased d-spacing). Stretch applied perpendicular to dominant fibril direction caused the fibrils to initially become less oriented as they were pulled away from the original direction, and less force was initially transmitted along the length of the fibrils (i.e., the d-spacing changed less). SAXS analysis of OFM and the starting raw tissue showed there is no difference in the structural arrangement of the collagen fibrils. Understanding the directional structural response of these materials under strain may influence how surgeons select and place the materials in use.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Collagen Fibril Response to Strain in Scaffolds from Ovine Forestomach for Tissue Engineering

Sizeland

Wells

Kelly

et al. 2017

ACS Biomater. Sci. Eng.

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“…Haemoglobin (%), Packed Cell Volume (%), Total Leukocyte Count (10 3 /mm 3 ), Differential Leucocytic Count, Alanine Aminotransferase (ALT-U/L), Creatine Kinase (CK-U/L), Gama-glutamyl transferase (GGT-U/L), Serum Cortisol (ng/ml), Serum Total Protein (g/dl) and Serum Albumin (g/ dl)was carried out on 0 th , 3 rd , 5 th , 7 th and 10 th day following surgical hernioplasty. The blood serum was subjected to indirect ELISA as per method described by Mohsina et al (2014) on 0 th , 5 th , 10 th , 15 th , 20 th and 30 th days of surgical reconstruction.…”

Section: Methodsmentioning

confidence: 99%

Preparation of Porcine Allogenic Acellular Diaphragm Matrix and its Application for Umbilical Hernioplasty in Pig

Nath

Sarma

2022

IJAR

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Background: The surgical reconstruction of large abdominal defects with application of surgical mesh of synthetic origin resulted a number of potential clinical complications; although it is having good tensile strength. Acellular bio-mesh derived from slaughtered animals could be better alternative for surgical management abdominal wall defects. The present study aimed to evaluate bio-detergent based decellularized allogenic porcine acellular diaphragm matrix in umbilical hernioplasty in pig. Methods: For the present study sodium deoxycholate was used as bio-detergent for preparation of porcine allogenic acellular diaphragm matrix (ADM). All total eighteen (18) numbers of piglets aged 2-3 months, body weight 8-14 kg and either sex with umbilical hernia equally divided in to three groups. Group A was subjected to application of ADM; while in Group B injection of methylprednisolone along with ADM application and Autologous Tunica Vaginalis was used for hernioplasty in Group C. Various parameters viz clinical, gross evaluation of surgical wound, haemato-biochemical and indirect ELISA of blood and serum was carried out as per standard methods. Result: Decellularization protocol carried out with sodium deoxycholate as bio-detergent@ 2% concentration resulted complete decellularization of porcine diaphragm and preserved the distinctive, natural, three-dimensional collagen structure within the prepared matrix. Clinical and haemato-biochemical observations following umbilical hernioplasty in pig with ADM promises its clinical utility. The immune response of host tissue to bio-mesh as revealed by Indirect ELISA was negligible.

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“…Compared to the other cell sources previously assessed, p‐MEF cells exhibit the advantage of being easily expandable from a single embryo and of being relatively inert immunologically (due to the absence of major histocompatibility complex class II antigen) . During the 60 days of the in vivo experiment, p‐MEF seeded scaffolds presented better adhesion results associated with histological signs of graft remodeling and tissue reconstruction, while eliciting low immunoreactivity …”

Section: Cell Coated Meshesmentioning

confidence: 96%

“…Since this preliminary investigation, improvement of tissue integration and graft vascularization were observed when ADSC and BM‐MSC were pre‐seeded on biological decellularized matrices. Alternatively, bioengineering scaffolds seeded with primary mouse embryo fibroblast (p‐MEF) cells have recently been introduced in the field of hernia regeneration . Compared to the other cell sources previously assessed, p‐MEF cells exhibit the advantage of being easily expandable from a single embryo and of being relatively inert immunologically (due to the absence of major histocompatibility complex class II antigen) .…”

Section: Cell Coated Meshesmentioning

confidence: 99%

Emerging Trends in Abdominal Wall Reinforcement: Bringing Bio‐Functionality to Meshes

Guillaume

Teuschl

Gruber-Blum

et al. 2015

Adv Healthcare Materials

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Abdominal wall hernia is a recurrent issue world-wide and requires the implantation of over 1 million meshes per year. Because permanent meshes such as polypropylene and polyester are not free of complications after implantation, many mesh modifications and new functionalities have been investigated over the last decade. Indeed, mesh optimization is the focus of intense development and the biomaterials utilized are now envisioned as being bioactive substrates that trigger various physiological processes in order to prevent complications and to promote tissue integration. In this context, it is of paramount interest to review the most relevant bio-functionalities being brought to new meshes and to open new avenues for the innovative development of the next generation of meshes with enhanced properties for functional abdominal wall hernia repair.

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Bioengineered acellular dermal matrices for the repair of abdominal wall defects in rats

Abstract: On the basis of the results bioengineered cell seeded scaffolds were found to be better than non-cell seeded scaffolds for the repair of abdominal wall defects in rats.

Cited by 11 publications

References 20 publications

Collagen Fibril Response to Strain in Scaffolds from Ovine Forestomach for Tissue Engineering

Collagen Fibril Response to Strain in Scaffolds from Ovine Forestomach for Tissue Engineering

Preparation of Porcine Allogenic Acellular Diaphragm Matrix and its Application for Umbilical Hernioplasty in Pig

Emerging Trends in Abdominal Wall Reinforcement: Bringing Bio‐Functionality to Meshes

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