Biodegradation of phenol by Ewingella americana: Effect of carbon starvation and some growth conditions

Khleifat, Khaled M.

doi:10.1016/j.procbio.2006.04.015

Cited by 87 publications

(66 citation statements)

References 27 publications

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“…They reported that the rate of phenol degradation is influenced by the energetic status or growth phase of the cells. The same findings were reported by Khleifat [15] for the degradation of phenol by Ewingella americana. According to the study, starvation increased the cells ability to utilize the organic substrate and the nutrients and decreased the lag time.…”

Section: Resultssupporting

confidence: 90%

“…According to the study, starvation increased the cells ability to utilize the organic substrate and the nutrients and decreased the lag time. Khleifat [15] explained that the quantity of the enzymes is the same but it is the time of early or late expression of catabolic genes that makes the difference. Additionally, a study by Varma and Gaikwad [24] for biodegradation of phenol by the yeast Candida tropicalis NCMI 3556 revealed that grown cells (already exiting the exponential phase) could degrade 100% of 2000 mg/l phenol whereas only 32% could be degraded when phenol was added to a medium with growing cells (still in the exponential phase).…”

Section: Resultsmentioning

confidence: 99%

“…The high-volume use of phenols in the United States and their potential toxicity has led the U.S. Environmental Protection Agency (US EPA) to define them as priority pollutants [15]. Most countries specify the maximum allowable concentration of phenols in the effluent streams to be less than 1 mg/l [16], [17].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transient Behavior in Biodegradation of 2, 4 Dichlorophenol: Is It a Starvation Effect?

Al-Khalid¹,

El‐Naas²

2013

IJCEA

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Abstract-Batch experiments were carried out to examine the biomass acclimatization during the aerobic biodegradation of 2, 4 dichlorophenol (2, 4 DCP) by Pseudomonas putida immobilized in polyvinyl alcohol (PVA) gel pellets in a bubble column bioreactor. The bacteria was acclimatized to 2, 4 DCP with concentrations of up to 200 mg/l, with and without the addition of glucose. After the acclimatization, experiments were carried out with initial 2, 4 DCP concentrations ranging from 25 to 100 mg/l. In the course of the study, a phenomenon was noticed that can be related to the effect of carbon starvation on the biodegradation ability of the bacterial cells. This paper presents the results on this phenomenon. Starved cells showed higher growth and degradation rates.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

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Transient Behavior in Biodegradation of 2, 4 Dichlorophenol: Is It a Starvation Effect?

Al-Khalid¹,

El‐Naas²

2013

IJCEA

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“…The biodegradation was tested using 5 different concentrations (1, 2, 3, 4 and 5 mM from 3,4-DCBA compound). The formation of any bacterial biomass will be a function of exhaustion of this substrates (Khleifat, 2006a(Khleifat, , 2006b(Khleifat, , 2006c The maximum level of degradation was obtained with 3mM 3,4-DCBA in 20-40 hr of incubation (Table 1). The growth reaches 0.26 OD at 600 nm.…”

Section: Effect Of Substrate Concentrationmentioning

confidence: 99%

Optimizing the Biodegradation of 3,4-Dichlorobenzoic Acid by Corynebacterium jeikeium

Alqudah¹,

Tarawneh²,

Alkafaween³

et al. 2014

IJB

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Chlorinated benzoic acids (CBA) have been intentionally released to the environment due to their use in agriculture as herbicides or pesticides, or unintentionally because they are common metabolites in the aerobic transformation of many chlorinated pollutants. The biodegradation of 3,4-dichlorobenzoic acid compound was investigated by using Corynebacterium jeikeium bacteria, which was isolated from Petra wastewater plant in Jordan. 3,4-dichlorobenzoic acid compound (3,4-DCBA) was used as a sole carbon and energy source in minimal salt media (MSM). 3,4-DCBA was the most degradable compound among the Chlorobenzoic acid compounds tested by this bacteria. Different conditions such as substrate concentration, temperature, pH, agitation rate, carbon starvation and carbon adaptation were used to obtain optimal Biodegradation. The optimal conditions for the biodegradation were 3mM as substrate concentration and the culture conditions were also showed a significant impact on the ability of these cells to remove 3,4-DCBA. The optimum solution, temperature and agitation rate were 7.5, 37 °C and 150 rpm, respectively. Adaptation of the cells to 3,4-DCBA for 24 hr and 48 hr and cells starvation for 24 hr and 48 hr increased the initial degradation rate. The degradation ability was monitored through disappearance of the substrate from the medium and the measuring the accompanying chloride release. Corynebacterium jeikeium dioxygenases were physiologically induced by 3,4-DCBA compound. They were analyzed for both ortho or meta ring-cleavage of this aromatic compound. Only 1,2-dioxygenase activity was detected which mean that the cleavage is through the ortho pathway. This microorganism can be a valuable and promising candidate for use in the biotreatment of wastewater samples contaminated with 3,4-DCBA.

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“…These microorganisms also modify their cell surface in order to increase the affinity for hydrophobic substrates and thus facilitate their absorption [9]. Toluene degradation is sensitive to many factors such as temperature [10], pH, incubation periods, carbon and nitrogen sources, and aeration rate [11]. Response surface methodology (RSM) is a collection of statistical and mathematical techniques for designing experiments, building models, evaluating the relative significance of several independent variables, and determining the optimum conditions for desirable responses [12][13].…”

Section: Introductionmentioning

confidence: 99%

Optimizing Toluene Degradation by Bacterial Strain Isolated from Oil-Polluted Soils

Heydarnezhad¹,

Hoodaji²,

Shahriarinour³

et al. 2018

Pol. J. Environ. Stud.

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The presence of toxic compounds like toluene has caused extensive contamination in oil-contaminated environments. Using bacteria to degrade monoaromatic compounds could be a good approach to finding a suitable bioaugmentation agent. In this study on toluene, degrading bacterial species were isolated from oil-contaminated environments (located in Bandar-Anzali, Guilan, Iran). The strain has been molecularly identified as Bacillus cereus ATHH39 (Accession number: KX344721) by partial sequencing of the 16S rDNA gene. Response surface methodology (RSM) was used for biodegradation of toluene by ATHH39 by implementing the central composite design (CCD). The central composite design (CCD) was applied to optimize and investigate pH, temperature, and toluene concentrations and their interactions for enhancing cell growth and toluene degradation by ATHH39 under in vitro conditions. The variables (pH, temperature, and toluene concentrations) with the highest significant impacts on growth and toluene degradation were selected. According to the prediction and optimization function of the design expert software, the optimum conditions of cell growth and toluene degradation were found. When pH, temperature, and toluene concentration were adjusted to 6.72, 33.16ºC and 824.15 mg/l, respectively, cell growth and toluene degradation reached OD 600 = 0.69 and 64.11%, respectively, which is very close to the predicted cell growth and toluene degradation of OD 600 = 0.71 and 65.85%, indicating that the response surface methodology optimization of process parameters for cell growth and toluene degradation is reliable. Based on the results, the ATHH39 strain was introduced as a useful microorganism with the potential for bioremediation of wastewater containing toluene.

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Biodegradation of phenol by Ewingella americana: Effect of carbon starvation and some growth conditions

Cited by 87 publications

References 27 publications

Transient Behavior in Biodegradation of 2, 4 Dichlorophenol: Is It a Starvation Effect?

Transient Behavior in Biodegradation of 2, 4 Dichlorophenol: Is It a Starvation Effect?

Optimizing the Biodegradation of 3,4-Dichlorobenzoic Acid by Corynebacterium jeikeium

Optimizing Toluene Degradation by Bacterial Strain Isolated from Oil-Polluted Soils

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