Transient Behavior in Biodegradation of 2, 4 Dichlorophenol: Is It a Starvation Effect?

Al-Khalid, Taghreed; El‐Naas, Muftah H.

doi:10.7763/ijcea.2013.v4.326

Cited by 3 publications

(7 citation statements)

References 24 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The positive influence of starvation on degradation activity was shown earlier in respect of this culture, R. opacus 1CP [28] as well as P. putida P300 degrading chlorophenols [62]. Cells of P. putida, immobilized in polyvinyl alcohol gel pellets in a bubble column bioreactor showed higher growth and degradation rates against 2,4-dichlorophenol after starvation compared to vegetative cells [62]. Low carbon content was shown to enhance the arsenic oxidation ability of the strains across different genera in Proteobacteria [63].…”

Section: Monitoring Phenol Degradation By Immobilized Versus Free Cellssupporting

confidence: 71%

“…Low carbon content was shown to enhance the arsenic oxidation ability of the strains across different genera in Proteobacteria [ 63 ]. A strong co-relation between carbon starvation and arsenic oxidation ability [ 63 ], and the positive influence of starvation on the degradation activity of a number of bacteria [ 28 , 62 , 64 ] provide an important basis for designing effective biopreparations for bioremediation of contaminated environments.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Removal of Phenol by Rhodococcus opacus 1CP after Dormancy: Insight into Enzymes’ Induction, Specificity, and Cells Viability

Egozarian,

Emelyanova,

Suzina

et al. 2024

Microorganisms

View full text Add to dashboard Cite

Biodegradation of phenol is an effective method for removing this toxicant from contaminated sites. Phenol is a toxic compound for living cells, so many bacteria degrade phenol in relatively low concentrations, up to 0.75 g L−1. The Rhodococcus opacus strain 1CP is an effective destructor of a wide range of pollutants. In the absence of a carbon source in the medium, cells of the R. opacus 1CP strain easily form cyst-like resting cells (CLC). The purpose of this work was to evaluate the viability of cells during long-term storage and the efficiency of the process of phenol destruction by R. opacus 1CP cells germinating after dormancy. Resting cells were obtained by simple cultivation in a rich medium followed by storage under static conditions. This is a simple approach to obtain a large amount of biomass. Decomposition of phenol proceeded via catechol followed by ortho-cleavage of aromatic ring. The induction of three phenol hydroxylases was detected by RT-PCR in cells germinated in a mineral medium with phenol as the carbon source. The stability of the genome of cells germinating after dormancy is shown by box-PCR. Dormant R. opacus 1CP cells, both suspended and immobilized, can be directly used for the decomposition of phenol after 4–12 months storage. In addition to phenol, after 9 months of storage, immobilized germinating cells easily metabolized 4-chlorophenol and 2,4,6-trichlorophenol. The results demonstrate a potential and simple approach toward achieving long-term storage of cells for further use in bioremediation.

show abstract

Section: Monitoring Phenol Degradation By Immobilized Versus Free Cellssupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Removal of Phenol by Rhodococcus opacus 1CP after Dormancy: Insight into Enzymes’ Induction, Specificity, and Cells Viability

Egozarian,

Emelyanova,

Suzina

et al. 2024

Microorganisms

View full text Add to dashboard Cite

show abstract

“…to be 1 µg/L in drinking water as per a guideline of the World Health Organization (WHO) (Al-Khalid & El-Naas, 2014;Yan et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Also, biological treatment methods are more acceptable because there are chances for complete mineralization of Phenolic compounds (Al-Khalid & El-Naas, 2014;El-Naas et al, 2009;Liu et al, 2009). Das and Dey (2020) degraded P-nitrophenol by using Pseudomonas sp.…”

Section: Introductionmentioning

confidence: 99%

“…Chlorophenols (Mono, Di, or Tri‐chlorophenol) mainly affect human nervous system and very persistent in nature possessing very low biodegradability (Armenante et al., 1999; Garba et al., 2019; Gupta et al., 2009; Tan et al., 2009). For these adverse impacts of the phenolic compounds on living creatures as well as human beings, maximum permissible concentration of phenolic compounds has been decided to be 1 μg/L in drinking water as per a guideline of the World Health Organization (WHO) (Al‐Khalid & El‐Naas, 2014; Yan et al., 2006).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and acclimatization of the most potential 4‐chlorophenol degrading bacterial strain isolated from hazardous soil and observing its performance in various process parameters

Maity,

Kundu,

Adhikari (Nee Pramanik)

2023

Environmental Quality Mgmt

View full text Add to dashboard Cite

Abstract4‐Chlorophenol is present as an essential compound of various organic compounds found in different kinds of hazardous waste. It can be easily eliminated from the industrial effluents by different physical and chemical treatment methods although best technique is bioremediation. To separate a potent bacterial strain from the soil that can dispose of 4‐chlorophenol from contaminated water, soil sample was collected from a hospital zone. Isolated bacterial colonies were segregated by the technique of soil enrichment with 500 mg/L 4‐chlorophenol and after that the enriched soil sample was serially diluted and transferred to petri plates with agar media. Twenty‐seven colonies were isolated and the most potent strain was selected in 50 mL liquid medium that was enriched previously with 500 mg/L of 4‐chlorophenol. C19 was found to be the most potent strain and it had the ability to reduce almost 99.97% of 4‐chlorophenol within 24 h, 37°C temperatures as well as in shaking condition (140–150 rpm) and pH 6.8–7.2. After the isolation, the strain was acclimatized in mineral salt medium (MSM) from lower concentration to higher concentration of 4‐chlorophenol to increase its removal capacity as well as to prepare the inoculums. Then parameter optimization study was performed where optimum temperature and pH was found to be 25°C and 6.5°C, respectively, 400 mL media volume and 8% inoculums were found to be most effective and finally 24 h time period was optimized. All these five optimum parameters were applied together to remove higher conc. of 4‐chlorophenol where almost 99.3% removals were obtained when the conc. was 600 mg/L. Moreover, more than 90% removals were obtained in case of 700, 800, and 900 mg/L concentration. Morphological, biochemical, nucleotide homology, and phylogenetic investigation of the strain was made which revealed that it was a rod shaped (Bacillus type), gram +ve, spore forming and motile bacterial strain and therefore, the selected strain was found to exhibit most extreme similarities (91.73%) with Bacillus cereus strain MK789657.

show abstract

Microbial Degradation of Chlorophenols

El‐Naas

Mousa

Gamal

2016

Microbe-Induced Degradation of Pesticides

View full text Add to dashboard Cite

Transient Behavior in Biodegradation of 2, 4 Dichlorophenol: Is It a Starvation Effect?

Cited by 3 publications

References 24 publications

Removal of Phenol by Rhodococcus opacus 1CP after Dormancy: Insight into Enzymes’ Induction, Specificity, and Cells Viability

Removal of Phenol by Rhodococcus opacus 1CP after Dormancy: Insight into Enzymes’ Induction, Specificity, and Cells Viability

Identification and acclimatization of the most potential 4‐chlorophenol degrading bacterial strain isolated from hazardous soil and observing its performance in various process parameters

Microbial Degradation of Chlorophenols

Contact Info

Product

Resources

About