Optimizing Toluene Degradation by Bacterial
Strain Isolated from Oil-Polluted Soils

Heydarnezhad, Fatemeh; Hoodaji, Mehran; Shahriarinour, Mahdi; Tahmourespour, Arezoo; Shariati, Shahab

doi:10.15244/pjoes/75811

Cited by 19 publications

(10 citation statements)

References 23 publications

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“…Thus, the three bacteria could form a co-metabolic relationship. Studies reported that the degradation of BTX by a combination of P. putida, B. cereus and B. subtilis was superior to that by a single bacterium 34,35,37 , which was consistent with the results of this study. In recent years, fungi have been reported to degrade BTX effectively.…”

Section: Treatment Effect Of the High-efficiency Microbial Communitysupporting

confidence: 93%

“…The laccase contained in the P. putida can oxidise the benzene ring to produce phenols or cyclohexenol 32,33 . B. cereus has a high ability to degrade toluene 34 , while B. subtilis has a better ability to degrade benzene than toluene and xylene 35 and easily causes the ring opening reaction of benzene ring 36 . Thus, the three bacteria could form a co-metabolic relationship.…”

Section: Treatment Effect Of the High-efficiency Microbial Communitymentioning

confidence: 99%

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Combination of highly efficient microflora to degrade paint spray exhaust gas

Lan

Yang

et al. 2020

Sci Rep

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Spray paint exhaust gas contains recalcitrant volatile organic compounds (VOCs), such as benzene, toluene and xylene (BTX). Treating BTX with a biofilter often achieves unsatisfactory results because the biofilter lacks efficient microbial community. In this work, three strains for BTX degradation were isolated and identified as Pseudomonas putida, Bacillus cereus and Bacillus subtilis by using 16S rRNA sequencing technology. A consortium of highly efficient microbial community was then constructed on a stable biofilm to treat BTX in a biofilter. A relatively suitable ratio of P. putida, B. cereus and B. subtilis was obtained. An efficiency of over 90% was achieved in the biofilter with VOC concentration of 1000 mg/m 3 through inoculation with the microbial community after only 10 days of operation. Thus, fast start-up of the biofilter was realised. Analysis of intermediate products by gas chromatographymass spectrometry indicated that BTX was degraded into short-chain aldehydes or acids via ring opening reactions. Paint spray exhaust gas includes volatile organic compounds (VOCs), such as benzene, toluene and xylene (BTX), and even more complicated compounds, i.e. chlorinated benzenes and toluenes 1,2 , all of which can cause great harm to humans 3,4. The indiscriminate discharge of large amounts of paint spray exhaust gas also exerts a negative impact on the atmospheric environment 5,6. Therefore, efficient technologies should be developed to treat paint spray exhaust gas and address its effects. Although several technologies for paint spray exhaust gas treatment exist, some of these methods are difficult to apply on a large scale because of their shortcomings 7. Biofiltration and biotrickling filters have received wide attention because they are low-cost and occupy a small space. Moreover, the activity of microorganisms can remain comparatively stable 8-10. Leili et al. found that the biofiltration system shows good performance in terms of removing BTEX 11. In theory, VOCs can completely mineralise during biofiltration. However, it is restricted by the microorganism type and the operating parameters in practise. Thus, the effect remains unsatisfactory 12. The toxicants in waste gas could be degraded with a specific strain by acclimation, which can be applied to the biofilters to improve their efficiency. A removal efficiency of higher than 98% was achieved by Bacillus firmus when the concentration of ketone (acetone and methyl ethyl ketone) and benzene in paint spray exhaust gas was lower than 3000 mg/m 3 13. Mohammad et al. used Exophiala sp. as inoculum for biofilter, which had a good removal effect on BTEX 14. However, because the composition of the exhaust gas might be complex, a single strain may not always obtain satisfactory results, resulting in unstable removal rates 15,16. The microbial community has been studied to promote biofilter operation. Archaea and bacteria effectively remove printing press VOCs in an anaerobic bioscrubber 17. Xue et al. used a biotrickling filter to treat complex odorous gas...

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Section: Treatment Effect Of the High-efficiency Microbial Communitysupporting

confidence: 93%

Section: Treatment Effect Of the High-efficiency Microbial Communitymentioning

confidence: 99%

Combination of highly efficient microflora to degrade paint spray exhaust gas

Lan

Yang

et al. 2020

Sci Rep

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“…A study on BTEX biodegradation 26 indicated that a pH 6-8 was optimum for BTEX biodegradation and the degradation was inhibited at pH 5, 9 and 10. A bacterial strain Bacillus cereus ATHH39 was selected as a potent toluene degrading organism and the conditions for better degradation were optimized as pH 6.72, 33.16°C, and toluene concentration of 824.15 mg/l through response surface methodology 27 .…”

Section: Fourier Transform Infrared Spectroscopy (Ft/ir) Analysis Ofmentioning

confidence: 99%

A Statistical Tool for the Optimization of Parameters for the Degradation of Mono-aromatic Pollutants by A Formulated Microbial Consortium

Vijayan¹

2019

J Pure Appl Microbiol

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The population rise and industrialization has resulted in the uncontrolled and untreated discharges of various toxic chemicals from different industries and these toxic chemicals cause's very serious pollution problems. Microbial degradation of toxic pollutants is one of the important way to remove the environmentally harmful compounds. The microorganisms metabolize or enzymatically transform the chemicals to less toxic metabolites. The present research emphasized on the biodegradation of mono-aromatic pollutants like Benzene, Toluene, Xylene and Phenol (BTXP) by a formulated microbial consortium constituted by Alcaligenes sp d 2 , Enterobacter aerogenes, Raoultella sp and Bacillus megaterium. Statistical design tools have been used for the optimization of different parameters which influencing BTXP biodegradation. The influencing parameters pH, concentration of BTXP and inoculum formulation was identified using Plackett-Burman design and was fine-tuned with Response Surface Methodology. The results specified that pH 6.25 and 250µl of 5% BTXP with the consortium formulated by mixing equal proportions ie, 3 ml of all the four bacterial isolates with OD 1,were found to be optimum for biodegradation. The degradation efficiency of the formulated consortium at its optimized condition was analyses by Fourier Transform Infrared Spectroscopy (FT/IR). About 96% of BTXP was degraded from the medium at the optimized condition and the spectral changes in FT/IR also recommended the effective removal of toxic chemicals from the medium.

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“…Although microbial growth in Jet A-1 storage tanks can cause a variety of serious problems, it is reasonable to suppose that microbes that are able to proliferate at such high hydrocarbon concentrations might be powerful candidates for bioremediation (bioaugmentation) processes [2,3].…”

Section: Introductionmentioning

confidence: 99%

Jet A-1 Bacterial Contamination: a Case Study of Cultivable Bacteria Diversity, Alkane Degradation and Biofilm Formation

Chiciudean¹,

Mereuță²,

Ionescu³

et al. 2019

Pol. J. Environ. Stud.

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Bacterial contamination during fuel storage can lead to fuel biodegradation (biodeterioration) or infrastructure problems (i.e., microbiologically influenced corrosion (MIC), biofouling, etc.). Jet A-1 storage tank samples were screened, and 23 morphologically distinct bacterial strains were isolated. 6 isolates revealed biofilm initiation capacity. For 12 isolates the alkane monooxygenase gene (alkB) was successfully detected. The 12 alkB-possessing isolates were 16S rDNA sequences identified as belonging to Bradyrhizobium, Sphingomonas, Bacillus, Pseudomonas, Methylobacterium and Lelliottia genera. In addition to possessing enzymatic equipment (AlkB) required for alkane oxidation, 10 out of 12 isolates were able to use Jet A-1 and n-tetradecane as their sole carbon source and energy. Biofilm initiation capacity and the ability to grow on different hydrocarbons highlights once again that fuel bacterial contamination can lead to serious fuel and storage system alterations. In contrast, their adaptation to high concentrations of hydrocarbons highlights the potential use of our isolates for bioremediation processes.

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Optimizing Toluene Degradation by Bacterial Strain Isolated from Oil-Polluted Soils

Cited by 19 publications

References 23 publications

Combination of highly efficient microflora to degrade paint spray exhaust gas

Combination of highly efficient microflora to degrade paint spray exhaust gas

A Statistical Tool for the Optimization of Parameters for the Degradation of Mono-aromatic Pollutants by A Formulated Microbial Consortium

Jet A-1 Bacterial Contamination: a Case Study of Cultivable Bacteria Diversity, Alkane Degradation and Biofilm Formation

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