Biodegradation of pharmaceuticals by Rhodococcus rhodochrous and Aspergillus niger by co-metabolism

Gauthier, H.; Yargeau, Viviane; Cooper, David G.

doi:10.1016/j.scitotenv.2009.12.012

Cited by 168 publications

(79 citation statements)

References 18 publications

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“…(Bouju et al 2012). Batch experiments were also conducted using pure bacterial cultures in an aqueous matrix consisting of minimum mineral salt medium in order to determine the SMXdegrading efficacy of specific bacterial strains that have been previously isolated from biological WW treatment systems (Gauthier et al 2010;Larcher and Yargeau 2011). One study focused on the ability of Rhodococcus rhodochrous and observed 20 % SMX degradation only in the presence of glucose (30 mg/L SMX+3 g/L glucose); SMX was not removed when it was the sole carbon source.…”

Section: Success Of Individual Microorganismsmentioning

confidence: 99%

“…One study focused on the ability of Rhodococcus rhodochrous and observed 20 % SMX degradation only in the presence of glucose (30 mg/L SMX+3 g/L glucose); SMX was not removed when it was the sole carbon source. Several degradation products were detected, and the most probable identity (based on LC-MS analysis) of the one with the greatest stability and highest concentration was hydroxy-N-(5-methyl-1,2-oxazol-3-yl)benzene-1-sulfonamide (Gauthier et al 2010). The second study investigated the SMX-degrading abilities of seven individual strains of bacteria in the presence and absence of glucose (6 mg/L SMX± 0.5 g/L glucose): Bacillus subtilis, Pseudomonas aeruginosa, Pseudomonas putida, Rhodococcus equi, Rhodococcus erythropolis, Rhodococcus rhodochrous, and Rhodococcus zopfii.…”

Section: Success Of Individual Microorganismsmentioning

confidence: 99%

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Biodegradation of sulfamethoxazole: current knowledge and perspectives

Larcher

Yargeau

2012

Appl Microbiol Biotechnol

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113

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Antibiotic compounds, like sulfamethoxazole (SMX), have become a concern in the aquatic environment due to the potential development of antibacterial resistances. Due to extensive consumption, excretion and disposal, SMX has been frequently detected in wastewaters and surface waters. This has led to numerous studies investigating the nature of SMX, with many researchers focusing on the biodegradation and persistence of SMX during wastewater treatment and in the environment. This review provides a summary of recent developments, outlines the discrepancies in observations and results, and demonstrates the need for further research to determine optimal biological removal strategies for SMX and other antibiotics.

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Section: Success Of Individual Microorganismsmentioning

confidence: 99%

Section: Success Of Individual Microorganismsmentioning

confidence: 99%

Biodegradation of sulfamethoxazole: current knowledge and perspectives

Larcher

Yargeau

2012

Appl Microbiol Biotechnol

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113

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“…Most studies on SMX biodegradation assessed its removal by activated sludge [14][15][16][17][18] and only few studies identified the bacterial strains involved in the process. The first examples were reported for members of the genus Rhodococcus, which were able to co-metabolize SMX with glucose in axenic culture [19,20]. Later, other organisms, all enriched from activated sludge, belonging to the genera Achromobacter, Ralstonia, Pseudomonas, Brevundimonas, Variovorax, Rhodococcus, and Microbacterium were reported as capable of degrading SMX [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of sulfamethoxazole and other sulfonamides by Achromobacter denitrificans PR1

Reis

Ricken

et al. 2014

Journal of Hazardous Materials

169

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This study aimed to isolate and characterize a microbial culture able to degrade sulfonamides. Sulfamethoxazole (SMX)-degrading microorganisms were enriched from activated sludge and wastewater. The resultant mixed culture was composed of four bacterial strains, out of which only Achromobacter denitrificans PR1 could degrade SMX. This sulfonamide was used as sole source of carbon, nitrogen and energy with stoichiometric accumulation of 3-amino-5-methylisoxazole. Strain PR1 was able to remove SMX at a rate of 73.6 ± 9.6 µmolSMX/gcell dry weight h. This rate more than doubled when a supplement of amino acids or the other members of the mixed culture were added. Besides SMX, strain PR1 was able to degrade other sulfonamides with anti-microbial activity. Other environmental Achromobacter spp. could not degrade SMX, suggesting that this property is not broadly distributed in members of this genus. Further studies are needed to shed additional light on the genetics and enzymology of this process.

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“…were identified, which were not further degraded (16)(17)(18). Most commercial sulfonamides are para-substituted aromatic amines.…”

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confidence: 99%

ipso -Hydroxylation and Subsequent Fragmentation: a Novel Microbial Strategy To Eliminate Sulfonamide Antibiotics

Ricken

Corvini

Cichocka

et al. 2013

Appl Environ Microbiol

106

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f Sulfonamide antibiotics have a wide application range in human and veterinary medicine. Because they tend to persist in the environment, they pose potential problems with regard to the propagation of antibiotic resistance. Here, we identified metabolites formed during the degradation of sulfamethoxazole and other sulfonamides in Microbacterium sp. strain BR1. Our experiments showed that the degradation proceeded along an unusual pathway initiated by ipso-hydroxylation with subsequent fragmentation of the parent compound. The NADH-dependent hydroxylation of the carbon atom attached to the sulfonyl group resulted in the release of sulfite, 3-amino-5-methylisoxazole, and benzoquinone-imine. The latter was concomitantly transformed to 4-aminophenol. Sulfadiazine, sulfamethizole, sulfamethazine, sulfadimethoxine, 4-amino-N-phenylbenzenesulfonamide, and N-(4-aminophenyl)sulfonylcarbamic acid methyl ester (asulam) were transformed accordingly. Therefore, ipso-hydroxylation with subsequent fragmentation must be considered the underlying mechanism; this could also occur in the same or in a similar way in other studies, where biotransformation of sulfonamides bearing an amino group in the para-position to the sulfonyl substituent was observed to yield products corresponding to the stable metabolites observed by us. Sulfonamides are widely used as antibiotics, antidiabetics, diuretics, antivirals, and anticancer agents (1-4), and thus, large amounts of these compounds enter the environment every year (5, 6). Contamination with sulfonamides poses environmental concern due to the potential development and dissemination of antibiotic resistances (7). Despite their ubiquity, their microbial metabolism and ultimate fate in the environment thereof are poorly understood.Several studies showed that sulfamethoxazole (SMX) (Fig. 1a), an important representative of sulfonamide compounds, undergoes partial degradation in wastewater treatment plants under aerobic and anaerobic conditions (8-11). We recently demonstrated that Microbacterium sp. strain BR1, a Gram-positive bacterium isolated from a membrane bioreactor treating effluent contaminated by several pharmaceuticals, was capable of mineralizing the 14 C-labeled aniline moiety of SMX when the latter was supplied as the sole carbon source (12). This was the first unambiguous indication that sulfonamides are subject to growth-linked metabolism in microorganisms.To our knowledge, Hartig (13) was the first to identify the aminated heteroaromatic side moieties of the sulfonamides SMX and sulfadimethoxine as stable metabolites after biodegradation with activated sludge. This result was recently confirmed by two groups that were able to isolate Microbacterium strains with the ability to degrade the sulfonamides sulfamethazine (SMZ) (14) and sulfadiazine (SDZ) (15). Additionally, both groups identified the aminated heteroaromatic side moieties of the sulfonamide as a stable metabolite after the degradation of the parent compound. Although these stable metabolites were identified, the i...

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Biodegradation of pharmaceuticals by Rhodococcus rhodochrous and Aspergillus niger by co-metabolism

Cited by 168 publications

References 18 publications

Biodegradation of sulfamethoxazole: current knowledge and perspectives

Biodegradation of sulfamethoxazole: current knowledge and perspectives

Biodegradation of sulfamethoxazole and other sulfonamides by Achromobacter denitrificans PR1

ipso -Hydroxylation and Subsequent Fragmentation: a Novel Microbial Strategy To Eliminate Sulfonamide Antibiotics

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