Biodegradable matrices from regenerated silk of Bombix mori

Агапов, И. И.; Moisenovich, Mikhail M.; Vasilyeva, T. V.; Pustovalova, O. L.; Kon’kov, A. S.; Arkhipova, A. Yu.; Sokolova, Olga S.; Богуш, В. Г.; Sevastianov, V. I.; Дебабов, В. Г.; Kirpichnikov, M. P.

doi:10.1134/s1607672910040149

Cited by 10 publications

(3 citation statements)

References 11 publications

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“…For recombinant spidroin, the alternation of short hydrophobic and hydrophilic regions is characteristic, which allows the surface of the microgel to exhibit either hydrophobic or hydrophilic properties, depending on the environment. F/G MPs were obtained by cryodestruction of spongy scaffolds (21, 31). The resulting F/G MPs are the fragments of a scaffold with a complex surface, providing a large surface area for cell adhesion and proliferation.…”

Section: Resultsmentioning

confidence: 99%

Novel Biodegradable Polymeric Microparticles Facilitate Scarless Wound Healing by Promoting Re-epithelialization and Inhibiting Fibrosis

et al. 2018

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Despite decades of research, the goal of achieving scarless wound healing remains elusive. One of the approaches, treatment with polymeric microcarriers, was shown to promote tissue regeneration in various in vitro models of wound healing. The in vivo effects of such an approach are attributed to transferred cells with polymeric microparticles functioning merely as inert scaffolds. We aimed to establish a bioactive biopolymer carrier that would promote would healing and inhibit scar formation in the murine model of deep skin wounds. Here we characterize two candidate types of microparticles based on fibroin/gelatin or spidroin and show that both types increase re-epithelialization rate and inhibit scar formation during skin wound healing. Interestingly, the effects of these microparticles on inflammatory gene expression and cytokine production by macrophages, fibroblasts, and keratinocytes are distinct. Both types of microparticles, as well as their soluble derivatives, fibroin and spidroin, significantly reduced the expression of profibrotic factors Fgf2 and Ctgf in mouse embryonic fibroblasts. However, only fibroin/gelatin microparticles induced transient inflammatory gene expression and cytokine production leading to an influx of inflammatory Ly6C+ myeloid cells to the injection site. The ability of microparticle carriers of equal proregenerative potential to induce inflammatory response may allow their subsequent adaptation to treatment of wounds with different bioburden and fibrotic content.

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Section: Resultsmentioning

confidence: 99%

Novel Biodegradable Polymeric Microparticles Facilitate Scarless Wound Healing by Promoting Re-epithelialization and Inhibiting Fibrosis

et al. 2018

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“…In this regard, the study of the biological properties of silk fibroin from cocoons of the silkworm Bombyx mori and the development of scaffolds based on it are of particular interest [12]. Silk fibroin has a unique combination of properties and can be used in many tissue engineering areas, both alone and in composites [13,14].…”

Section: Introductionmentioning

confidence: 99%

A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin

et al. 2021

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A comparative analysis of the structure and biological properties of silk fibroin constructions was performed. Three groups of constructions were obtained: films obtained by casting an aqueous solution of silk fibroin and electrospun microfibrous scaffolds based on silk fibroin, with the addition of 30% gelatin per total protein weight. The internal structures of the films and single fibers of the microfibrous scaffolds consisted of densely packed globule structures; the surface area to volume ratios and volume porosities of the microfibrous scaffolds were calculated. All constructions were non-toxic for cells and provide high levels of adhesion and proliferation. The high regenerative potential of the constructions was demonstrated in a rat full-thickness skin wound healing model. The constructions accelerated healing by an average of 15 days and can be considered to be promising constructions for various tasks of tissue engineering and regenerative medicine.

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“…Pores connected with holes and channels form a complex, unclosed internal surface that facilitates cell migration to the internal layers of an artificial scaffold. Furthermore, an unclosed pore structure provides conditions for the medium exchange and removal of metabolites, thus facilitating the formation of a homogenous intra-scaffold medium [5, 7-9]. …”

Section: Resultsmentioning

confidence: 99%

Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

et al. 2014

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Three-dimensional (3D) silk fibroin scaffolds were modified with one of the major bone tissue derivatives (nano-hydroxyapatite) and/or a collagen derivative (gelatin). Adhesion and proliferation of mouse embryonic fibroblasts (MEF) within the scaffold were increased after modification with either nano-hydroxyapatite or gelatin. However, a significant increase in MEF adhesion and proliferation was observed when both additives were introduced into the scaffold. Such modified composite scaffolds provide a new and better platform to study wound healing, bone and other tissue regeneration, as well as artificial organ bioengineering. This system can further be applied to establish experimental models to study cell-substrate interactions, cell migration and other complex processes, which may be difficult to address using the conventional two-dimensional culture systems.

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Biodegradable matrices from regenerated silk of Bombix mori

Cited by 10 publications

References 11 publications

Novel Biodegradable Polymeric Microparticles Facilitate Scarless Wound Healing by Promoting Re-epithelialization and Inhibiting Fibrosis

Novel Biodegradable Polymeric Microparticles Facilitate Scarless Wound Healing by Promoting Re-epithelialization and Inhibiting Fibrosis

A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin

Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

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