Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

Moisenovich, M. M.; Arkhipova, A. Yu.; Орлова, А. А.; Drutskaya, Marina S.; Volkova, S. V.; Zacharov, S E; Агапов, И. И.; Kirpichnikov, Mikhaǐl P.

doi:10.32607/20758251-2014-6-1-96-101

Cited by 30 publications

(16 citation statements)

References 15 publications

(20 reference statements)

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“…The report demonstrated that silk fibroin scaffolds needed special modifications to enhance the biological performance to induce the formation of bone tissue . Therefore, modified silk fibroin scaffolds with decellularized pulp and fibronectin were presented in this research.…”

Section: Discussionmentioning

confidence: 97%

“…The report demonstrated that silk fibroin scaffolds needed special modifications to enhance the biological performance to induce the formation of bone tissue. 47 Therefore, modified silk fibroin scaffolds with decellularized pulp and fibronectin were presented in this research. The aim of modification is to mimic the microenvironment of decellularized pulp/fibronectin that can enhance the biological performance of silk fibroin scaffolds such as cell adhesion and proliferation.…”

Section: Biological Performance Of Modified Silk Fibroin Scaffoldsmentioning

confidence: 99%

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Modified porous scaffolds of silk fibroin with mimicked microenvironment based on decellularized pulp/fibronectin for designed performance biomaterials in maxillofacial bone defect

Sangkert

Kamonmattayakul

Chai

et al. 2017

J Biomedical Materials Res

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Maxillofacial bone defect is a critical problem for many patients. In severe cases, the patients need an operation using a biomaterial replacement. Therefore, to design performance biomaterials is a challenge for materials scientists and maxillofacial surgeons. In this research, porous silk fibroin scaffolds with mimicked microenvironment based on decellularized pulp and fibronectin were created as for bone regeneration. Silk fibroin scaffolds were fabricated by freeze-drying before modification with three different components: decellularized pulp, fibronectin, and decellularized pulp/fibronectin. The morphologies of the modified scaffolds were observed by scanning electron microscopy. Existence of the modifying components in the scaffolds was proved by the increase in weights and from the pore size measurements of the scaffolds. The modified scaffolds were seeded with MG-63 osteoblasts and cultured. Testing of the biofunctionalities included cell viability, cell proliferation, calcium content, alkaline phosphatase activity (ALP), mineralization and histological analysis. The results demonstrated that the modifying components organized themselves into aggregations of a globular structure. They were arranged themselves into clusters of aggregations with a fibril structure in the porous walls of the scaffolds. The results showed that modified scaffolds with a mimicked microenvironment of decellularized pulp/fibronectin were suitable for cell viability since the cells could attach and spread into most of the pores of the scaffold. Furthermore, the scaffolds could induce calcium synthesis, mineralization, and ALP activity. The results indicated that modified silk fibroin scaffolds with a mimicked microenvironment of decellularized pulp/fibronectin hold promise for use in tissue engineering in maxillofacial bone defects. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1624-1636, 2017.

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Section: Discussionmentioning

confidence: 97%

Section: Biological Performance Of Modified Silk Fibroin Scaffoldsmentioning

confidence: 99%

Modified porous scaffolds of silk fibroin with mimicked microenvironment based on decellularized pulp/fibronectin for designed performance biomaterials in maxillofacial bone defect

Sangkert

Kamonmattayakul

Chai

et al. 2017

J Biomedical Materials Res

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“…Cell proliferation-related genes were identified at both time points in peri-implant bone healing. As previously mentioned, cell proliferation is a key biological process in tissue development and regeneration [ 36 , 37 ]. The present study identified several genes, such as CDK14 , PLCD1 , and ZBTB16 , which were upregulated during peri-implant bone healing.…”

Section: Discussionmentioning

confidence: 99%

Gene Profiling of Bone around Orthodontic Mini-Implants by RNA-Sequencing Analysis

Nahm

Heo

Lee

et al. 2015

BioMed Research International

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This study aimed to evaluate the genes that were expressed in the healing bones around SLA-treated titanium orthodontic mini-implants in a beagle at early (1-week) and late (4-week) stages with RNA-sequencing (RNA-Seq). Samples from sites of surgical defects were used as controls. Total RNA was extracted from the tissue around the implants, and an RNA-Seq analysis was performed with Illumina TruSeq. In the 1-week group, genes in the gene ontology (GO) categories of cell growth and the extracellular matrix (ECM) were upregulated, while genes in the categories of the oxidation-reduction process, intermediate filaments, and structural molecule activity were downregulated. In the 4-week group, the genes upregulated included ECM binding, stem cell fate specification, and intramembranous ossification, while genes in the oxidation-reduction process category were downregulated. GO analysis revealed an upregulation of genes that were related to significant mechanisms, including those with roles in cell proliferation, the ECM, growth factors, and osteogenic-related pathways, which are associated with bone formation. From these results, implant-induced bone formation progressed considerably during the times examined in this study. The upregulation or downregulation of selected genes was confirmed with real-time reverse transcription polymerase chain reaction. The RNA-Seq strategy was useful for defining the biological responses to orthodontic mini-implants and identifying the specific genetic networks for targeted evaluations of successful peri-implant bone remodeling.

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“…Зарубежные компании придают большое значение усовершенствованию имеющихся материалов и созданию новых полимеров для возможной имплантации в организм человека, в связи возросшим спросом на данный вид продукции. В зависимости от области применения изготовляемых продуктов, помимо высоких биосовместимых свойств, пластики должны соответствовать другим немаловажным критериям [21]. Пластмассы, использующиеся для изготовления зубных имплантатов, ортопедических протезов, должны обладать достаточно прочностными качествами.…”

Section: D-биопринтерыunclassified

Использование 3d-Принтеров В Хирургии (Обзор Литературы)

Лазаренко¹,

Иванов²,

Ivanov³

et al. 2018

Курск. науч.-практ. вестн. «Человек и его здоровье»

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Развитие 3D-технологий, повсеместное распространение и усовершенствование 3D-принтеров, а также разработка и применение новых материалов и их комбинаций способствовали широкому внедрению 3D-принтеров в медицину. Использование 3D-технологий в хирургии возможно по следующим направлениям. Во-первых, это создание различных имплантатов, широко применяемых в стоматологии, травматологии и реконструктивной хирургии. Во-вторых, использование 3D-моделей подходит для имитации хода операций в сложных случаях. В-третьих, полным ходом идет разработка сканирования и печати органов и тканей живыми клетками, так называемый биопринтинг. В четвертых, существует опыт применения 3D-принтеров для создания медицинских инструментов. И наконец, активно исследуется использование 3D-принтеров для создания различных эндопротезов. Таким образом, перспективы 3D-технологий огромны, и в ближайшем будущем они, несомненно, станут одним из самых динамичных направлений в медицине и в хирургии в частности.

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Composite Scaffolds Containing Silk Fibroin, Gelatin, and Hydroxyapatite for Bone Tissue Regeneration and 3D Cell Culturing

Cited by 30 publications

References 15 publications

Modified porous scaffolds of silk fibroin with mimicked microenvironment based on decellularized pulp/fibronectin for designed performance biomaterials in maxillofacial bone defect

Modified porous scaffolds of silk fibroin with mimicked microenvironment based on decellularized pulp/fibronectin for designed performance biomaterials in maxillofacial bone defect

Gene Profiling of Bone around Orthodontic Mini-Implants by RNA-Sequencing Analysis

Использование 3d-Принтеров В Хирургии (Обзор Литературы)

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