Bioconversion of residual glycerol from biodiesel synthesis into 1,3-propanediol using immobilized cells of Klebsiella pneumoniae BLh-1

Souza, Elisangela Aquino de; Rossi, Daniele Misturini; Ayub, Marco Antônio Záchia

doi:10.1016/j.renene.2014.07.030

Cited by 35 publications

(8 citation statements)

References 18 publications

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“…Previous reports in the literature stated the inhibitory effects of high concentrations of the substrate on wild type microorganisms [20]. In order to overcome this issue and enhance the titers of 1,3-PDO and other metabolites, strategies such as acclimation of the microorganisms with the formation of biofilms [32], immobilization on inert materials , entrapment of cells in gels [33] or fed-batch mode of processing have been employed [5,34,35]. Similarly to the reported literature, at least 6 cycles of OLR was also successfully applied to the biofilm of C. freundii DSM 15979 cells in the present study to enhance the productivities (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Production of Value-Added Bioproducts Using a Modified Continuous Biofilm Reactor by Citrobacter Freundii DSM 15979

Güngörmüşler¹

2021

Hittite J. Sci. Eng.

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C ommodity chemicals are derived largely from raw materials based on fossil resources. The environmentally friendly biotechnological approaches potentially form same or functionally similar chemicals from sustainable materials. In addition, their effects on reduction in greenhouse gas emissions provide an additional advantage. Various industrial byproducts and domestic waste materials can be used as raw materials to convert into value-added chemicals by means of fermentative pathways. Numerous studies reported the technical viability of valorization of biodiesel driven glycerol into 1,3-PDO, 2,3-BD, ethanol, butanol, H2, acetic acid, lactic acid and other organic acids (Fig. 1). The versatility of the final byproduct of this conversion is due to the utilization of different metabolic pathways by numerous species including Klebsiella pneumoniae [1], Citrobacter freundii [2-6], Pantoea agglomerans [7], Entrobacter aerogenes [8], Lactobacillus brevis [9], Lactobacillus diolivorans [10], Lactobacillus reuteri [11], Clostridium butyricum [12], Clostridium perfringens [13], Clostridium acetobutylicum [14], Clostridium paste-Article History:

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Section: Resultsmentioning

confidence: 99%

Production of Value-Added Bioproducts Using a Modified Continuous Biofilm Reactor by Citrobacter Freundii DSM 15979

Güngörmüşler¹

2021

Hittite J. Sci. Eng.

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“…However, the group without activated carbon accumulated lipid and formed a resting body in the third cycle, which proved that activated carbon provided a favorable microenvironment for cells. Similarly, after immobilization with calcium alginate, K. pneumoniae BLh‐1 exhibited a 1.52‐fold increase in 1,3‐PDO productivity in batch culture compared with suspended cells 119 . More important, the immobilized cells could maintain operational stability and biological activity in five cycles of repeated batch culture, and the 1,3‐PDO production productivity still reached 4.48 g (L·h) −1 .…”

Section: 3‐pdo Production By Using Mono‐culturementioning

confidence: 92%

Current advances in microbial production of 1,3‐propanediol

Zhu

Wang

Gao

et al. 2021

Biofuels Bioprod Bioref

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1,3‐Propanediol (1,3‐PDO), an important chemical raw material, has attracted much attention due to its wide use as a monomer of biodegradable polyester materials. Production of 1,3‐PDO from renewable resources, through microbial fermentation, has the advantage of requiring only mild conditions. The process also demonstrates industrial safety and is environmentally friendly. In this review, we have summarized and commented on current progress in screening and breeding strains for 1,3‐PDO production, especially from glycerol or glucose. Advanced strategies, including metabolic engineering, fermentation process optimization, and construction of a microbial consortium system, are introduced and discussed. Current challenges and prospects for more efficient and economical bio‐based 1,3‐PDO production are examined in the context of its industrial application. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…This is a versatile strain, which has shown the ability to grow under extreme condition, including high osmotic pressure 36 . Further data on its isolation and characterization can be found elsewhere 6,33,37 Microbial viable stocks of the bacterium are part of the BiotecLab Microbiology Culture Collection (UFRGS, Brazil) and are kept frozen in 50% glycerol‐Luria–Bertani (LB) medium at −80°C. All chemicals used in this research were purchased from Sigma‐Aldrich (MO, St. Louis) and were of analytical grade.…”

Section: Methodsmentioning

confidence: 99%

“…Immobilized cells are particularly interesting to be used in continuous bioreactors, 20–24 or under repeated batch systems, in which the biocatalysts and cells are reused 33,34 . Most commonly used continuous bioreactor configurations used with immobilized cells are the tubular fluidized and fixed‐bed (packed‐bed) reactors systems 35 …”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of 1,3‐propanodiol and 2,3‐butanodiol from residual glycerol in continuous cell‐immobilized Klebsiella pneumoniae bioreactors

Damasceno

Rossi

Ayub

2022

Biotechnology Progress

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In recent years, residual glycerol from biodiesel synthesis made this chemical a cheap, readily available carbon source to bioprocess, which is also a form to reduce costs in the fuel industry. We propose and describe a bioprocess using fluidized and packedbed continuous bioreactors to convert this residual glycerol into value-added products such as 1,3-propanediol (1,3-PD) and 2,3-butanediol (2,3-BD), largely used in the chemical industry. The bacterium Klebsiella pneumoniae BLh-1, strain isolated by us, was immobilized in the permeable support of polyvinyl alcohol (LentiKats ® ). After testing different dilution rates (D) for all bioreactor configurations, the best obtained productivities of 1,3-PD was 8.69 g L À1 h À1 at a D = 0.45 h À1 , and 2.99 g L À1 h À1 at a D = 0.30 h À1 for 2,3-BD, both in the packed-bed configuration. In the fluidizedbed reactor, the highest productivity values achieved were 4.48 and 1.16 g L À1 h À1 for 1,3-PD and 2,3-BD, respectively, both at D = 0.33 h À1 . These results show the potential of setting up a bioprocess based on continuous cultures using immobilized K. pneumoniae BLh-1 in PVA matrices in order to efficiently convert the abundant surplus of glycerol into commercially important chemicals such as 1,3-PD and 2,3-BD.

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Bioconversion of residual glycerol from biodiesel synthesis into 1,3-propanediol using immobilized cells of Klebsiella pneumoniae BLh-1

Cited by 35 publications

References 18 publications

Production of Value-Added Bioproducts Using a Modified Continuous Biofilm Reactor by Citrobacter Freundii DSM 15979

Production of Value-Added Bioproducts Using a Modified Continuous Biofilm Reactor by Citrobacter Freundii DSM 15979

Current advances in microbial production of 1,3‐propanediol

Biosynthesis of 1,3‐propanodiol and 2,3‐butanodiol from residual glycerol in continuous cell‐immobilized Klebsiella pneumoniae bioreactors

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