Production of Value-Added Bioproducts Using a Modified Continuous Biofilm Reactor by Citrobacter Freundii DSM 15979

Abstract

C ommodity chemicals are derived largely from raw materials based on fossil resources. The environmentally friendly biotechnological approaches potentially form same or functionally similar chemicals from sustainable materials. In addition, their effects on reduction in greenhouse gas emissions provide an additional advantage. Various industrial byproducts and domestic waste materials can be used as raw materials to convert into value-added chemicals by means of fermentative pathways. Numerous studies reported the technical viability of valorization of biodiesel driven glycerol into 1,3-PDO, 2,3-BD, ethanol, butanol, H2, acetic acid, lactic acid and other organic acids (Fig. 1). The versatility of the final byproduct of this conversion is due to the utilization of different metabolic pathways by numerous species including Klebsiella pneumoniae [1], Citrobacter freundii [2-6], Pantoea agglomerans [7], Entrobacter aerogenes [8], Lactobacillus brevis [9], Lactobacillus diolivorans [10], Lactobacillus reuteri [11], Clostridium butyricum [12], Clostridium perfringens [13], Clostridium acetobutylicum [14], Clostridium paste-Article History:

Biosynthesis of 1,3‐propanodiol and 2,3‐butanodiol from residual glycerol in continuous cell‐immobilized Klebsiella pneumoniae bioreactors

“…There are some reports in the literature using continuous cultures in tubular reactors to produce 1,3‐PD with immobilized cells 26,45,46 . De Souza used immobilized K. pneumoniae BLh‐1 cells in calcium alginate and the reactors operated at a D = 0.05 h −1 .…”

“…There are some reports in the literature using continuous cultures in tubular reactors to produce 1,3-PD with immobilized cells. 26,45,46 De Souza used immobilized K. pneumoniae BLh-1 cells in calcium alginate and the reactors operated at a D = 0.05 h À1 . The authors reported best conditions to produce 1,3-PD using the fluidized-bed bioreactor compared against the packed-bed bioreactor.…”