Bioconversion of Agro-Industrial Waste to Value-Added Product Lycopene by Photosynthetic Bacterium Rhodopseudomonas faecalis and Its Carotenoid Composition

Patthawaro, Sirada; Lomthaisong, Khomsorn; Saejung, Chewapat

doi:10.1007/s12649-018-00571-z

Cited by 30 publications

(13 citation statements)

References 51 publications

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“…Glycerol and corn steep liquor (CSL) have attracted increasing interest as raw materials for microbial fermentative production of value-added chemicals and compounds. − Crude glycerol, which is generated as a by-product during biodiesel production, has been recognized as a useful carbon source for the cost-effective production of chemicals and compounds by the fermentation process. , Owing to its reduced form, it can easily produce reduced compounds by providing more equivalents compared with glucose. Furthermore, CSL has been regarded as an inexpensive substrate for the efficient production of target molecules because it contains large amounts of organic nitrogen, vitamins, and trace elements. − Previously, lycopene production of 71.25 mg/L/day was reported through the cultivation of photosynthetic bacterium Rhodopseudomonas faecalis using various agro-industrial wastes such as soybean, coconut, and cassava meal as substrates . In another experiment, microbial production of more than 2 g/L of carotene was achieved by the cultivation of B.…”

Section: Introductionmentioning

confidence: 99%

High-Yield Production of Lycopene from Corn Steep Liquor and Glycerol Using the Metabolically Engineered Deinococcus radiodurans R1 Strain

Kang

Jeong

Yang³

et al. 2020

J. Agric. Food Chem.

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Developing a highly efficient and ecofriendly system to produce desired products from waste can be considered important to a sustainable society. Here, we report for the first time high-yield production of lycopene through metabolically engineering an extremophilic microorganism, Deinococcus radiodurans R1, from corn steep liquor (CSL) and glycerol. First, the crtLm gene-encoding lycopene cyclase was deleted to prevent the conversion of lycopene to γ-carotene. Then, the crtB gene-encoding phytoene synthase and the dxs gene-encoding 1-deoxy-d-xylulose 5-phosphate synthase were overexpressed to increase carbon flux toward lycopene. The engineered ΔcrtLm/crtB + dxs + D. radiodurans R1 could produce 273.8 mg/L [80.7 mg/g dry cell weight (DCW)] and 373.5 mg/L (108.0 mg/g DCW) of lycopene from 10 g/L of glucose with 5 g/L of yeast extract and 9.9 g/L of glucose with 20 g/L of CSL, respectively. Moreover, the lycopene titer and content were increased by 26% (470.6 mg/L) and 28% (138.2 mg/g DCW), respectively, when the carbon source was changed to glycerol. Finally, fed-batch fermentation of the final engineered strain allowed the production of 722.2 mg/L (203.5 mg/g DCW) of lycopene with a yield and productivity of 20.3 mg/g glycerol and 6.0 mg/L/h, respectively, from 25 g/L of CSL and 35.7 g/L of glycerol.

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Section: Introductionmentioning

confidence: 99%

High-Yield Production of Lycopene from Corn Steep Liquor and Glycerol Using the Metabolically Engineered Deinococcus radiodurans R1 Strain

Kang

Jeong

Yang³

et al. 2020

J. Agric. Food Chem.

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“…Resource recovery studies should focus on utilizing low-cost non-toxic organic substrates like wastewater from the soybean meal, olive mill, sugar and molasses industry [ 135 , 136 ]. The upstream processing of substrate can be optimized in several ways to lower overall cost [ 250 ].…”

Section: Discussionmentioning

confidence: 99%

“…By exploiting carbon rich 2nd generation feedstock such as: waste, glycerol and volatile fatty acids from biodiesel and waste processing industries, PHA production can become more economical [ 134 ]. Non-toxic organic substrates like wastewater from the soybean meal, cheese whey, olive mill, sugar, molasses industry [ [135] , [136] , [137] ]; as well as carbon-rich lignocellulosic wastes such as spent coffee and waste from the paper and food processing industries [ 138 ] have also been studied for PHA production. Padovani et al [ 139 ] have demonstrated the ability of Rhodopseudomonas sp.…”

Section: Resource Recovery Applications Of Anoxygenic Phototrophic Bamentioning

confidence: 99%

“…Carotenoids such as lycopene 1,2-dihydrolycopene, cis-1,2-dihydrolycopene and 1,2-dihy- dro-3,4-dedihydrolycopene were synthesized. Rhodopseudomonas faecalis PA2 [ 136 ] Additives -yeast Artificial brewery wastewater Natural light and micro-aerobic conditions without aeration Yeast extract at 400 mg/L was the best for the growth of the bacteria, and at this concentration of yeast the carotenoid production was 2.53 mg/g. The addition of 400 mg/L yeast increased dehydrogenase activity, which was indicative of biomass growth [ 224 ].…”

Section: Resource Recovery Applications Of Anoxygenic Phototrophic Bamentioning

confidence: 99%

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An overview of anoxygenic phototrophic bacteria and their applications in environmental biotechnology for sustainable Resource recovery

George

Vincent

Mackey

2020

Biotechnology Reports

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Highlights APB are phylogenetically and metabolically diverse, includes many extremophiles. Ability to harvest IR light and use of many inorganic electron donors useful. Electricity, polymers, fertilizers, feed, antioxidants and pigments recoverable. Purple non-sulfur bacteria well studied, have wide range of applications. Metabolic engineering key to improving productivity and metabolic traits.

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“…Some of the recent reports on bacterial pigments using agro-industrial wastes highlighted the use of Erwinia uredova, Planococcus sp., and Rhodopseudomonas faecalis. These bacteria have demonstrated their potential in the production of carotenoid-type pigments using different agro-industrial wastes [8][9][10]. Moreover, Chryseobacterium artocarpi was able to synthesize yellowish-orange pigments using liquid pineapple waste [11].…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of potato solid waste into antifungals and biopigments using Streptomyces spp.

et al. 2021

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Potato waste was processed and used as a sole substrate for simultaneously producing antifungals and biopigments using Streptomyces spp. Out of three different Streptomyces isolates, strain SO6 stood out due to its ability to produce antifungals against economically important fungal phytopathogens and intracellular biopigments using potato waste powders without additional nutrients. This strain also showed the potential to secrete a broad range of enzymes for fermentation of eight sugars that could be involved in potato waste bioconversion. The results of the fermentation assay indicated that Streptomyces sp. strain SO6 degrades potato wastes during submerged fermentation, diminishing total dry weight and increasing reducing sugars from 0.3 to 3.6 mg·mL−1 and total proteins from 70.6 to 187.7 μg·mL−1. The results showed that Streptomyces strain SO6 was able to convert the potato waste into 0.96 mg·g−1 of diffusible antifungals and 1.75 mg·g−1 of reddish-purple biopigments. On the contrary, an absence of pigment production was observed during the fermentation of the commercial medium used as reference. According to our results, replacement of commercial culture media with available low-cost agroindustrial wastes for producing bioactive chemicals is a real opportunity to enhance the Streptomyces pigment production and antibiotic sustainability with cost-competitiveness. To our knowledge, this is the first report on the simultaneous production of biopigments and diffusible antifungal antibiotics produced by Streptomyces spp. using potato solid waste as the sole nutrient source.

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Bioconversion of Agro-Industrial Waste to Value-Added Product Lycopene by Photosynthetic Bacterium Rhodopseudomonas faecalis and Its Carotenoid Composition

Cited by 30 publications

References 51 publications

High-Yield Production of Lycopene from Corn Steep Liquor and Glycerol Using the Metabolically Engineered Deinococcus radiodurans R1 Strain

High-Yield Production of Lycopene from Corn Steep Liquor and Glycerol Using the Metabolically Engineered Deinococcus radiodurans R1 Strain

An overview of anoxygenic phototrophic bacteria and their applications in environmental biotechnology for sustainable Resource recovery

Bioconversion of potato solid waste into antifungals and biopigments using Streptomyces spp.

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