Bioconversion of potato solid waste into antifungals and biopigments using Streptomyces spp.

Abstract

Potato waste was processed and used as a sole substrate for simultaneously producing antifungals and biopigments using Streptomyces spp. Out of three different Streptomyces isolates, strain SO6 stood out due to its ability to produce antifungals against economically important fungal phytopathogens and intracellular biopigments using potato waste powders without additional nutrients. This strain also showed the potential to secrete a broad range of enzymes for fermentation of eight sugars that could be involved in potato waste bioconversion. The results of the fermentation assay indicated that Streptomyces sp. strain SO6 degrades potato wastes during submerged fermentation, diminishing total dry weight and increasing reducing sugars from 0.3 to 3.6 mg·mL−1 and total proteins from 70.6 to 187.7 μg·mL−1. The results showed that Streptomyces strain SO6 was able to convert the potato waste into 0.96 mg·g−1 of diffusible antifungals and 1.75 mg·g−1 of reddish-purple biopigments. On the contrary, an absence of pigment production was observed during the fermentation of the commercial medium used as reference. According to our results, replacement of commercial culture media with available low-cost agroindustrial wastes for producing bioactive chemicals is a real opportunity to enhance the Streptomyces pigment production and antibiotic sustainability with cost-competitiveness. To our knowledge, this is the first report on the simultaneous production of biopigments and diffusible antifungal antibiotics produced by Streptomyces spp. using potato solid waste as the sole nutrient source.

Streptomyces spp. as biocatalyst sources in pulp and paper and textile industries: Biodegradation, bioconversion and valorization of waste

“…has recently opened a door for the valorization of microbial‐generated products from food waste. For example in a recent, study by Schalchli et al ( 2021 ) Streptomyces spp. were used to produce antifungals and biopigments from potato solid waste.…”

A review on waste valorization, biotechnological utilization, and management of potato

“…The capacity of strain SO6 to produce intracellular biopigments and antifungals against commercially important fungal phytopathogens using powdered potato waste without the addition of additional nutrients made it stand out among the three different Streptomyces isolates. This strain also shows the capacity to release several enzymes for the fermentation of eight sugars that may be involved in the bioconversion of potato trash (Schalchli et al, 2021 ). The only substrate for the concurrent generation of antifungals and biopigments was prepared and supplied to Streptomyces spp.…”

“…Out of three separate Streptomyces isolates, strain SO6 stood out due to its capacity to produce intracellular biopigments and antifungals against commercially important fungal phytopathogens using powdered potato waste without the addition of additional nutrients. Also, this strain showed the capacity to release a range of enzymes for the fermentation of eight sugars that may be involved in the bioconversion of potato waste (Schalchli et al, 2021 ). Making pigment from M. purpureus Went NRRL 1992 using potato waste powder has some advantages.…”

Colorful Treasure From Agro-Industrial Wastes: A Sustainable Chassis for Microbial Pigment Production

“…The use of glycerol, a by-product of biodiesel manufacture as a carbon source by R. glutinis TISTR 5159 led to concomitant production of lipids (6.05 g/L) as well as carotenoids (135.25 mg/L) in a stirred tank bioreactor ( Saenge et al, 2011 ). Among the pioneer reports on biopigment production by Streptomyces genus utilizing agro-industrial wastes, Schalchli et al (2021) reported 1.75 mg/g pigment production by Streptomyces sp. strain SO6 utilizing potato waste as the sole nutrient source.…”