High-Yield Production of Lycopene from Corn Steep Liquor and Glycerol Using the Metabolically Engineered <i>Deinococcus radiodurans</i> R1 Strain

Kang, Chang Keun; Jeong, Sang‐Hun; Yang, Jung Eun; Choi, Yong Jun

doi:10.1021/acs.jafc.0c01024

Cited by 28 publications

(30 citation statements)

References 28 publications

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“…As can be seen in Figs. 2A and 2B, during the early exponential growth phase (12 h), lycopene content tended to rapidly increase up to 33.4 ± 1.3 mg/g DCW, but no significant increase was observed in the mid-exponential growth phase (24 h), which is consistent with our previous study [6]. In addition, the expression of the idi (isopentenyl diphosphate isomerase gene) and crtE (geranylgeranyl diphosphate synthase gene) genes, which encode the major rate-limiting enzymes in the carotenoid biosynthetic pathway [5,7,23], was lower at 24 h than at 12 h (Fig.…”

Section: Determination Uv Irradiation Onset By Comparative Analysis Osupporting

confidence: 91%

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Enhanced Lycopene Production by UV-C Irradiation in Radiation-Resistant Deinococcus radiodurans R1

Kang

Yang

Park

et al. 2020

J. Microbiol. Biotechnol.

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Although classical metabolic engineering strategies have succeeded in developing microbial strains capable of producing desired bioproducts, metabolic imbalance resulting from extensive genetic manipulation often leads to decreased productivity. Thus, abiotic strategies for improving microbial production performance can be an alternative to overcome drawbacks arising from intensive metabolic engineering. Herein, we report a promising abiotic method for enhancing lycopene production by UV-C irradiation using a radiation-resistant ΔcrtLm/crtB + dxs + Deinococcus radiodurans R1 strain. First, the onset of UV irradiation was determined through analysis of the expression of 11 genes mainly involved in the carotenoid biosynthetic pathway in the ΔcrtLm/crtB + dxs + D. radiodurans R1 strain. Second, the effects of different UV wavelengths (UV-A, UV-B, and UV-C) on lycopene production were investigated. UV-C irradiation induced the highest production, resulting in a 69.9% increase in lycopene content [64.2 ± 3.2 mg/g dry cell weight (DCW)]. Extended UV-C irradiation further enhanced lycopene content up to 73.9 ± 2.3 mg/g DCW, a 95.5% increase compared to production without UV-C irradiation (37.8 ± 0.7 mg/g DCW).

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Section: Determination Uv Irradiation Onset By Comparative Analysis Osupporting

confidence: 91%

“…The extracts were analyzed using a high-performance liquid chromatography (HPLC) system (Agilent 1260 InfinityII, Agilent Corporation, USA) equipped with a Zorbax Eclipse XDB-C18 column (4.6 × 250 mm, Agilent, USA) as described previously [6].…”

Section: Methodsmentioning

confidence: 99%

Enhanced Lycopene Production by UV-C Irradiation in Radiation-Resistant Deinococcus radiodurans R1

Kang

Yang

Park

et al. 2020

J. Microbiol. Biotechnol.

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“…We then overexpressed DXS in this strain, resulting in strain WT P-I-D, which was expected to increase the production of 1-deoxy- d -xylulose-5- phosphate (DXP) from pyruvate (Pyr) and 3-glyceraldehyde phosphate (GA3P), and thereby increase the flux through the non-mevalonate pathway. It has previously been shown that among the enzymes of this pathway, only overexpression of this enzyme can increase the production of GPP and carotenoids in D. radiodurans significantly [ 32 ], but in our experiments, it did not lead to a significantly higher pinene titer.…”

Section: Resultscontrasting

confidence: 73%

Metabolic engineering of Deinococcus radiodurans for pinene production from glycerol

et al. 2021

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Background The objective of this work was to engineer Deinococcus radiodurans R1 as a microbial cell factory for the production of pinene, a monoterpene molecule prominently used for the production of fragrances, pharmaceutical products, and jet engine biofuels. Our objective was to produce pinene from glycerol, an abundant by-product of various industries. Results To enable pinene production in D. radiodurans, we expressed the pinene synthase from Abies grandis, the geranyl pyrophosphate (GPP) synthase from Escherichia coli, and overexpressed the native 1-deoxy-d-xylulose 5-phosphate synthase. Further, we disrupted the deinoxanthin pathway competing for the substrate GPP by either inactivating the gene dr0862, encoding phytoene synthase, or substituting the native GPP synthase with that of E. coli. These manipulations resulted in a D. radiodurans strain capable of producing 3.2 ± 0.2 mg/L pinene in a minimal medium supplemented with glycerol, with a yield of 0.13 ± 0.04 mg/g glycerol in shake flask cultures. Additionally, our results indicated a higher tolerance of D. radiodurans towards pinene as compared to E. coli. Conclusions In this study, we successfully engineered the extremophile bacterium D. radiodurans to produce pinene. This is the first study demonstrating the use of D. radiodurans as a cell factory for the production of terpenoid molecules. Besides, its high resistance to pinene makes D. radiodurans a suitable host for further engineering efforts to increase pinene titer as well as a candidate for the production of the other terpenoid molecules.

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“…CSL is a major byproduct of cornstarch processing and is a low-cost source of proteins, amino acids, minerals and vitamins. It has been reported to be a potential nitrogen source for the production of bioproducts 36 – 39 . The optimized medium developed in this study (containing crude glycerol, CSL, and feed-grade l -tryptophan) was more cost-effective than other media and achieved high IAA production with a less-expensive fermentation medium.…”

Section: Discussionmentioning

confidence: 99%

Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

Bunsangiam

Thongpae

Limtong

et al. 2021

Sci Rep

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Indole-3-acetic acid (IAA) is the most common plant hormone of the auxin class and regulates various plant growth processes. The present study investigated IAA production by the basidiomycetous yeast Rhodosporidiobolus fluvialis DMKU-CP293 using the one-factor-at-a-time (OFAT) method and response surface methodology (RSM). IAA production was optimized in shake-flask culture using a cost-effective medium containing 4.5% crude glycerol, 2% CSL and 0.55% feed-grade l-tryptophan. The optimized medium resulted in a 3.3-fold improvement in IAA production and a 3.6-fold reduction in cost compared with those obtained with a non-optimized medium. Production was then scaled up to a 15-L bioreactor and to a pilot-scale (100-L) bioreactor based on the constant impeller tip speed (Vtip) strategy. By doing so, IAA was successfully produced at a concentration of 3569.32 mg/L at the pilot scale. To the best of our knowledge, this is the first report of pilot-scale IAA production by microorganisms. In addition, we evaluated the effect of crude IAA on weed growth. The results showed that weed (Cyperus rotundus L.) growth could be inhibited by 50 mg/L of crude IAA. IAA therefore has the potential to be developed as a herbicidal bioproduct to replace the chemical herbicides that have been banned in various countries, including Thailand.

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High-Yield Production of Lycopene from Corn Steep Liquor and Glycerol Using the Metabolically Engineered Deinococcus radiodurans R1 Strain

Cited by 28 publications

References 28 publications

Enhanced Lycopene Production by UV-C Irradiation in Radiation-Resistant Deinococcus radiodurans R1

Enhanced Lycopene Production by UV-C Irradiation in Radiation-Resistant Deinococcus radiodurans R1

Metabolic engineering of Deinococcus radiodurans for pinene production from glycerol

Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

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