Biocontrol potential of Trichoderma harzianum isolate T-aloe against Sclerotinia sclerotiorum in soybean

Zhang, Fuli; Ge, Haixia; Zhang, Fan; Guo, Ning; Wang, Yucheng; Long, Chen; Xiu-e, JI; Li, Chengwei

doi:10.1016/j.plaphy.2015.12.017

Cited by 121 publications

(73 citation statements)

References 72 publications

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“…In contrast, we observed an increase in relative area dedicated to thylakoid structures in chloroplasts within PA23-treated plants coupled with an increase in total chlorophyll content and repression of CLH1. In other studies, BCA application has been associated with increased chlorophyll in the plant host [60–62]. The lack of overexpression of CLH1 in the PA23-only treatment group is consistent with evidence showing that CLH1 is only induced after cellular collapse, a defense response not elicited by nonpathogenic organisms [63].…”

Section: Discussionsupporting

confidence: 86%

The biocontrol agent Pseudomonas chlororaphis PA23 primes Brassica napus defenses through distinct gene networks

et al. 2017

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BackgroundThe biological control agent Pseudomonas chlororaphis PA23 is capable of protecting Brassica napus (canola) from the necrotrophic fungus Sclerotinia sclerotiorum via direct antagonism. While we have elucidated bacterial genes and gene products responsible biocontrol, little is known about how the host plant responds to bacterial priming on the leaf surface, including global changes in gene activity in the presence and absence of S. sclerotiorum.ResultsApplication of PA23 to the aerial surfaces of canola plants reduced the number of S. sclerotiorum lesion-forming petals by 91.1%. RNA sequencing of the host pathogen interface showed that pretreatment with PA23 reduced the number of genes upregulated in response to S. sclerotiorum by 16-fold. By itself, PA23 activated unique defense networks indicative of defense priming. Genes encoding MAMP-triggered immunity receptors detecting flagellin and peptidoglycan were downregulated in PA23 only-treated plants, consistent with post-stimulus desensitization. Downstream, we observed reactive oxygen species (ROS) production involving low levels of H2O2 and overexpression of genes associated with glycerol-3-phosphate (G3P)-mediated systemic acquired resistance (SAR). Leaf chloroplasts exhibited increased thylakoid membrane structures and chlorophyll content, while lipid metabolic processes were upregulated.ConclusionIn addition to directly antagonizing S. sclerotiorum, PA23 primes the plant defense response through induction of unique local and systemic defense networks. This study provides novel insight into the effects of biocontrol agents applied to the plant phyllosphere. Understanding these interactions will aid in the development of biocontrol systems as an alternative to chemical pesticides for protection of important crop systems.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3848-6) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 86%

The biocontrol agent Pseudomonas chlororaphis PA23 primes Brassica napus defenses through distinct gene networks

et al. 2017

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“…Triple superphosphate, a readily available source in the soil, presented the most promising result because the analysis was performed twenty days after the planting of the crop, a result that was already expected. Some authors have demonstrated the increase on chlorophyll level due the presence of Trichoderma on different cultures as cucumber, wheat, soybean and lettuce plants [59][60][61][62] .…”

Section: Discussionmentioning

confidence: 99%

Phosphorus-solubilizing Trichoderma spp. from Amazon soils improve soybean plant growth

Bononi

Chiaramonte

Pansa

et al. 2020

Sci Rep

123

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Acidic soils rapidly retain applied phosphorus fertilizers and consequently present low availability of this nutrient to plants. the use of phosphate-solubilizing microorganisms to help plant phosphorus (P) absorption is a promising sustainable strategy for managing P deficiencies in agricultural soils. Trichoderma strains have been one of the most studied filamentous fungi for improving the production and development of several crop species mainly due to their capability for symbiotic associations and their ability to control soil-borne plant diseases. Thus, this work sought to bioprospect Trichoderma strains from the Amazon rainforest capable of solubilizing/mineralizing soil phosphate and promoting soybean growth. Soybean plants inoculated with selected Trichoderma strains were cultivated in soil under greenhouse conditions and under a gradient of rock phosphate and triple superphosphate. As a result, 19.5% of the isolated Trichoderma strains were able to solubilize phosphate. In addition, those strains produced different organic acids during the solubilization process. Trichoderma spp. strains showed positive responses in the promotion of soybean growth-from 2.1% to 41.1%-as well as in the efficiency of P uptake-up to 141%. These results reveal the potential of Trichoderma spp. from the Amazon biome as promising biofertilizer agents.The high demand for fertilizers used in Brazilian agriculture is a result of the growing population, which necessitates an increase in food production 1 . Brazil is the second-largest supplier of food and agricultural products and expected to be the leading producer of food to meet global demand in the near future 2 . Thus, applications of fertilizer are a routine activity in agricultural production as an attempt to promote crop growth to increase productivity. The requirement of fertilizers in the field results in the accumulation of those inputs in soils and water and, therefore, environmental pollution, causing problems to human and animal health 1,3 . In the future, Brazilian agriculture has to identify alternatives to reduce its dependence on chemical fertilizers while at the same time functioning in a lucrative and more sustainable way 4 .A range of nutrients is important for plant growth 5 , but the ones that limit agricultural production the most are nitrogen and phosphorus, which are important in the initial development of the plant 6,7 . Nitrogen fertilization in Brazil has decreased significantly with use of symbiotic associations with nitrogen-fixing bacteria 8,9 . However, Brazilian agriculture continues to depend on chemical phosphate fertilization 4 . The role of P in the plant is associated with three essential biochemical processes: energy production, respiration, and photosynthesis. P is also involved in enzymatic processes and is a component of nucleic acids and cell membranes [10][11][12][13] . Phosphorus is generally found in the lowest concentration in the soil, 0.01%, compared to 0.14% of nitrogen, mainly in tropical and subtropical regions 14,15 . Although ...

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“…A relação de Trichoderma spp.com a planta ocorre, em geral, na região das raízes onde o fungo coloniza a epiderme e as células do córtex, onde uma série de mecanismos bioquímicos acionam transduções de sinais de respostas de defesa da planta contra patógenos diversos (35). Lamdan et al (17) verificaram que existem diversas proteínas secretadas por Trichoderma virens presentes quando em raízes de milho e ausente em sua ausência.…”

Section: Abstract: Biological Control Glycine Max White Mold Scannunclassified

“…Por fim, sua relação parasítica sobre fungos fitopatogênicos pode envolver eventos tais como localização, reconhecimento, contato direto, formação de estruturas em forma de gancho com função de apressórios, penetração, enovelamento e desenvolvimento de hifas paralelas, observados geralmente por meio de microscopia eletrônica de varredura (MEV) (1, 22,35).…”

Section: Abstract: Biological Control Glycine Max White Mold Scannunclassified

Emergência e análise ultraestrutural de plântulas de soja inoculadas com Sclerotinia sclerotiorum sob efeito da aplicação de Trichoderma harzianum

et al. 2017

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A elevada susceptibilidade da soja ao mofo-branco tem impactado negativamente a sua produtividade. Atualmente métodos de controle biológico como o uso de Trichoderma spp. têm proporcionado alternativas ao controle químico, com menor impacto ambiental e favorecimento do desenvolvimento vegetal. Objetivou-se avaliar os efeitos de T. harzianum sobre a emergência de sementes de soja inoculadas com Sclerotinia sclerotiorum; além de verificar a interação entre os fungos e com a soja, por meio da microscopia eletrônica de varredura (MEV ABSTRACTThe high susceptibility of soybean to white mold has negatively impacted its productivity. Currently, biological control methods, such as the use of Trichoderma spp., have provided alternatives to chemical control, as they promote less environmental impact and favor the plant growth. The aim of this study was to evaluate the effects of T. harzianum on the emergence of soybean seeds inoculated with Sclerotinia sclerotiorum, besides identifying the interaction between fungi and soybeans by means of scanning electron microscopy (SEM). Soybean seeds were subjected to the following treatments: 1. Without S. sclerotiorum, in PDA+Mannitol; 2. With S. sclerotiorum, in PDA+Mannitol; 3. With Ecotrich ® WP and without S. sclerotiorum, in PDA+Mannitol; 4. Silva, F.F.; Castro, E.M.; Moreira, S.I.; Ferreira, T.C.; Lima, A.E.; Alves, E. Emergence and ultrastructural analysis of soybean seedlings inoculated with Sclerotinia sclerotiorum under the effect of Trichoderma harzianum application. Summa Phytopathologica, v.43, n.1, p.41-45, 2017. With S. sclerotiorum and Ecotrich ® WP, in PDA+Mannitol. The emergence test was conducted on trays with sterile sand at 25 °C for 8 days. Then, the vegetative organs of emerged seedlings were sectioned to evaluate, by scanning electron microscopy (SEM), T. harzianum potential to parasitize and inhibit S. sclerotiorum. In fact, the white mold is capable of colonizing and deteriorating all soybean seeds. T. harzianum is effective in colonizing the root system of soybeans but does not contribute to emergence, compared to control. Based on the ultrastructural analysis, mycoparasitism of T. harzianum and S. Sclerotiorum was evidenced, but the control of the causal agent of white mold was not as effective as shown in other studies.

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Biocontrol potential of Trichoderma harzianum isolate T-aloe against Sclerotinia sclerotiorum in soybean

Cited by 121 publications

References 72 publications

The biocontrol agent Pseudomonas chlororaphis PA23 primes Brassica napus defenses through distinct gene networks

The biocontrol agent Pseudomonas chlororaphis PA23 primes Brassica napus defenses through distinct gene networks

Phosphorus-solubilizing Trichoderma spp. from Amazon soils improve soybean plant growth

Emergência e análise ultraestrutural de plântulas de soja inoculadas com Sclerotinia sclerotiorum sob efeito da aplicação de Trichoderma harzianum

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