Biocontrol of strawberry pathogen Botrytis cinerea using plant extracts and essential oils

Šernaitė, Lina; Rasiukevičiūtė, Neringa; Dambrauskienė, E.; Viškelis, Pranas; Valiuškaitė, Alma

doi:10.13080/z-a.2020.107.019

Cited by 32 publications

(13 citation statements)

References 34 publications

(26 reference statements)

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“…Dried cinnamon bark and clove bud were obtained from Saldva (Lithuania). Subcritical CO 2 extraction was performed while producing cinnamon and clove extracts as described in our previous study [44]. Both extracts were kept at 4 • C until the experiments.…”

Section: The Extraction Of Plant Materialsmentioning

confidence: 99%

The Extracts of Cinnamon and Clove as Potential Biofungicides against Strawberry Grey Mould

Šernaitė

Rasiukevičiūtė

Valiuškaitė

2020

Plants

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Biofungicides from plants are a possibility for the biocontrol of fungal diseases, as chemical products may be harmful to the environment and humans. Strawberry is one of the many plants infected by grey mould (Botrytis cinerea), and innovative methods of biocontrol against B. cinerea are under investigation. Clove (Syzygium aromaticum L.) and cinnamon (Cinnamomum cassia L.) accumulate natural compounds, such as eugenol and cinnamaldehyde, which provide antimicrobial and antifungal properties; thus, extracts of these plants could be possibly used as biofungicides. During this study, the inhibition of B. cinerea by clove and cinnamon extracts was evaluated in vitro on Petri plates and detached strawberry leaves; additionally, the chemical composition of volatiles was identified. Clove extract consisted of 52.88% eugenol, and cinnamon consisted of 74.67% cinnamaldehyde. The efficacy of the extracts on detached strawberry leaves showed that 12 mL L−1 concentration of clove extract was effective in suppressing the grey mould infection. Clove and cinnamon extracts showed an equal ability to inhibit B. cinerea on Petri plates. However, the results of the detached strawberry leaves assay showed that clove extract was more effective as a biocontrol product. Overall, clove extract expressed a high potential for application in biofungicides formulations.

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Section: The Extraction Of Plant Materialsmentioning

confidence: 99%

The Extracts of Cinnamon and Clove as Potential Biofungicides against Strawberry Grey Mould

Šernaitė

Rasiukevičiūtė

Valiuškaitė

2020

Plants

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“…EOs from a number of plants, including Eucalyptus (Eucalyptus camaldulensis), cinnamon (Cinnamomum ssp.) and cumin (Cuminum cyminum) could inhibit grey mould growth and increase the storage life and quality of strawberries [16,[25][26][27] and Botrytis spp. on peach and B. cinerea in eggplant [28].…”

Section: Introductionmentioning

confidence: 99%

Post-Harvest Enhancing and Botrytis cinerea Control of Strawberry Fruits Using Low Cost and Eco-Friendly Natural Oils

et al. 2021

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This work investigates an experimental study for using low-cost and eco-friendly oils to increase the shelf life of strawberry fruit. Three natural oils were used: (i) Eucalyptus camaldulensis var obtuse, (ii) Mentha piperita green aerial parts essential oils (EOs), and (iii) Moringa oleifera seeds n-hexane fixed oil (FO). Furthermore, a mixture of EOs from E. camaldulensis var obtusa and M. piperita (1/1 v/v) was used. The treated fruits were stored at 5 °C and 90% relative humidity (RH) for 18 days. HPLC was used to analyse the changes in phenolic compounds during the storage periods. The effects of biofumigation through a slow-release diffuser of EOs (E. camaldulensis var obtusa and M. piperita), or by coating with M. oleifera FO, were evaluated in terms of control of post-harvest visual and chemical quality of strawberry fruits. The post-harvest resistance of strawberry fruits to Botrytis cinerea fungal infection was also evaluated. As a result, the EO treatments significantly reduced the change in visual and chemical quality of strawberry fruit. Additionally, changes in the titratable acidity of moringa FO-coated strawberry fruits were delayed. EO treatments improved total soluble solids, total phenols, ascorbic acid, antioxidants and peroxidase. E. camaldulensis var obtusa and M. piperita (1/1 v/v) EO-vapour fruit exhibited a slower rate of deterioration, compared to other treatments in all tested, in two experiments. The lowest colour change (ΔE) was observed inthe fruit treated with E. camaldulensis var obtusa EO and M. oleifera FO. HPLC showed changes in phenolic compounds’ concentration, where p-coumaric acid, caffeic acid, gallic acid, ferulic acid and ellagic acid were mostly identified in the fruits treated with the oils. SEM examination confirmed the potential decrease in fungal growth as the fruits were treated with EOs. In conclusion, the treatment of EOs during different storage periods showed promising characterisations for strawberry fruit quality.

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“…Dried cinnamon bark ( Cinnamomum zeylanicum ), clove buds ( Syzygium aromaticum ) and laurel leaves ( Laurus nobilis ) were extracted using the CO 2 extraction method as described in the previous study [ 28 ]. Composition of the volatile compounds responsible for antifungal activities of each extract was determined previously using gas chromatography/mass spectrometry [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Sensitivity of Botrytis cinerea Isolates Complex to Plant Extracts

Dėnė

Valiuškaitė

2021

Molecules

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New agricultural strategies aim to reduce the use of pesticides due to their damage to the environment and humans, and the caused resistance to pathogens. Therefore, alternative sources of antifungal compounds from plants are under investigation lately. Extracts from plants have a wide composition of chemical compounds which may complicate the development of pathogen resistance. Botrytis cinerea, causing grey mould, is an important horticultural and ornamental pathogen, responsible for the relevant yield and quality losses. B. cinerea isolated from a different plant host may differ in the sensitivity to antifungal substances from plants. Assessing the importance of research covering a wide range of pathogens for the rapid development of biopesticides, this study aims to determine the sensitivity of the B. cinerea isolate complex (10 strains) to plant extracts, describe morphological changes caused by the extract treatment, and detect differences between the sensitivity of different plant host isolates. The results showed the highest sensitivity of the B. cinerea isolates complex to cinnamon extract, and the lowest to laurel extract. In contrast, laurel extract caused the most changes of morphological attributes in the isolates. Five B. cinerea isolates from plant hosts of raspberry, cabbage, apple, bell pepper, and rose were grouped statistically according to their sensitivity to laurel extract. Meanwhile, the bell pepper isolate separated from the isolate complex based on its sensitivity to clove extract, and the strawberry and apple isolates based on their sensitivity to cinnamon extract.

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Biocontrol of strawberry pathogen Botrytis cinerea using plant extracts and essential oils

Cited by 32 publications

References 34 publications

The Extracts of Cinnamon and Clove as Potential Biofungicides against Strawberry Grey Mould

The Extracts of Cinnamon and Clove as Potential Biofungicides against Strawberry Grey Mould

Post-Harvest Enhancing and Botrytis cinerea Control of Strawberry Fruits Using Low Cost and Eco-Friendly Natural Oils

Sensitivity of Botrytis cinerea Isolates Complex to Plant Extracts

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