We performed field experiments to evaluate the influence of two extraction treatments, seaweed (Pterocladia capillacea S.G. Gmelin) water extraction (WE) and ultrasound-assisted water extraction (USWE) at three concentrations (5%, 10%, and 15%), as well as control NPK traditional mineral fertilizer on the growth, yield, minerals, and antioxidants of Jew’s Mallow (Corchorus olitorius L.) during the two seasons of 2016 and 2017 in Egypt. Plant height, number of leaves, and fresh weight of WE10 treatment were the highest (p < 0.05) as 59.67 cm, 10.67 and 2.41 kg m−2 in 2016, respectively, and 57.33 cm, 11.00 and 2.32 kg m−2 in 2017, respectively. WE10 and USWE5 treatments produced the highest dry matter (17.07%) in 2016 and (16.97%) in 2017, respectively. WE10 plants had an increased water productivity of 41.2% relative to control plants in both seasons. The highest chlorophyll ‘a’ was recorded after the WE10 treatment in 2016 and 2017 (17.79 μg g−1 and 17.84 μg g−1, respectively). The highest levels of total antioxidant capacity, total phenolics, and total flavonoids were also recorded after the WE10 treatment. Application of WE10 boosted growth, yield, minerals, and antioxidants of Jew’s Mallow. The CROPWAT model was used to estimate the evapotranspiration, irrigation water requirements, and yield response to irrigation scheduling. Our data showed a yield reduction in the initial growth stage if a limited amount of water was provided. Therefore, irrigation water should be provided during the most important stages of crop development with the choice of effective irrigation practices to avoid water losses, as this helps to maximize yield.
This work investigates an experimental study for using low-cost and eco-friendly oils to increase the shelf life of strawberry fruit. Three natural oils were used: (i) Eucalyptus camaldulensis var obtuse, (ii) Mentha piperita green aerial parts essential oils (EOs), and (iii) Moringa oleifera seeds n-hexane fixed oil (FO). Furthermore, a mixture of EOs from E. camaldulensis var obtusa and M. piperita (1/1 v/v) was used. The treated fruits were stored at 5 °C and 90% relative humidity (RH) for 18 days. HPLC was used to analyse the changes in phenolic compounds during the storage periods. The effects of biofumigation through a slow-release diffuser of EOs (E. camaldulensis var obtusa and M. piperita), or by coating with M. oleifera FO, were evaluated in terms of control of post-harvest visual and chemical quality of strawberry fruits. The post-harvest resistance of strawberry fruits to Botrytis cinerea fungal infection was also evaluated. As a result, the EO treatments significantly reduced the change in visual and chemical quality of strawberry fruit. Additionally, changes in the titratable acidity of moringa FO-coated strawberry fruits were delayed. EO treatments improved total soluble solids, total phenols, ascorbic acid, antioxidants and peroxidase. E. camaldulensis var obtusa and M. piperita (1/1 v/v) EO-vapour fruit exhibited a slower rate of deterioration, compared to other treatments in all tested, in two experiments. The lowest colour change (ΔE) was observed inthe fruit treated with E. camaldulensis var obtusa EO and M. oleifera FO. HPLC showed changes in phenolic compounds’ concentration, where p-coumaric acid, caffeic acid, gallic acid, ferulic acid and ellagic acid were mostly identified in the fruits treated with the oils. SEM examination confirmed the potential decrease in fungal growth as the fruits were treated with EOs. In conclusion, the treatment of EOs during different storage periods showed promising characterisations for strawberry fruit quality.
Background: Natural plant extracts and microbial antagonists have the potential for use in increasing the fungal resistance and productivity of horticulture plants. Methods: The purpose of this study was to evaluate the ability of both natural plant extracts and microbial antagonists as a biotical control of some fungal pathogens, i.e., Fusarium ssp., Exserohilum ssp. and Nigrospora ssp., along with improving the growth and productivity performance of zucchini under greenhouse conditions. Eucalyptus camaldulensis leaf extract (LE), Citrus sinensis LE, Ficus benghalensis fruit extract (FE), and two microbial antagonists Pseudomonas fluorescens (accession no. MW647093) and Trichoderma viride (accession no. MW647090) were tested under in vitro and in vivo conditions. Through morphological characteristics and the internal transcribed spacer (ITS) region, Fusarium solani (accession no. MW947256), F. oxysporum (accession no. MW947254), Exserohilum rostratum (accession no. MW947255), and Nigrospora lacticolonia (accession no. MW947253) were identified. HPLC analysis was used for the identification of phenolic compounds (PCs) and flavonoid compounds (FCs) in the extracts. Results: The highest inhibition percentage of fungal growth (IPFG) against F. oxysporum was obtained with P. fluorescens, T. viride, and E. camaldulensis LE (4000 mg/L); F. solani with P. fluorescens, T. viride, and C. sinensis LE (4000 mg/L); Exserohilum rostratum with P. fluorescens, Ficus benghalensis FE (4000 mg/L) and E. camaldulensis LE (4000 mg/L), and N. lacticolonia with P. fluorescens. Using HPLC analysis, the abundant PCs in E. camaldulensis LE were pyrogallol, and caffeic acid, those in C. sinensis LE were syringic acid and ferulic acid, and those in F. benghalensis FE were gallic acid and syringic acid. In addition, the abundant FCs in E. camaldulensis LE were kaempferol, and naringin, those in C. sinensis LE were hesperidin and quercetin, and those in F. benghalensis FE were kaempferol and quercetin. Under greenhouse experiments, T. viride and E. camaldulensis LE (4000 mg/L) followed by P. fluorescens + T. viride treatments gave the best results of zucchini plants in terms of leaf area, fruits number per plant, yield per plant, and total yield (marketable and non-marketable). Conclusions: Plant extracts and bioagents can be used to control some zucchini fungal pathogens and increase the productivity performance of zucchini plants.
This study evaluated the effect of phosphorus and potassium (PK) fertilizer levels and foliar seaweed extract on early and total yield productivity and the growth of globe artichoke plants. Field experiments were conducted over two seasons on loamy–clay soil at the vegetable research farm, of the Faculty of Agriculture, Alexandria University, Egypt. Fertilizer levels of 0, 25, 50 and 75 mL L−1, and seaweed extract concentrations of 0, 5 and 10 mg L−1, individually and in combination, were used. Globe artichoke plants treated with PK liquid fertilizer, with and without seaweed extract, showed critical increases in growth (plant height and number of leaves per plant as well as foliage dry weight), yield, and some chemical constituents compared to untreated plants. The PK3 fertilizer level and 10 mL L−1 seaweed extract as a foliar spray showed greater effects than other combinations.
There are many technological innovations in the field of agriculture to improve the sustainability of farmed products by reducing the chemicals used. Uses of biostimulants such as plant extracts or microorganisms are a promising process that increases plant growth and the efficient use of available soil resources. To determine the effects of some biostimulants' treatments on the photosynthetic pigments and biochemicals composition of zucchini plants, two experiments were conducted in 2019 and 2020 under greenhouse conditions. In this work, the effects of beneficial microbes (Trichoderma viride and Pseudomonas fluorescens), as well as three extracts from Eucalyptus camaldulensis leaf extract (LE), Citrus sinensis LE, and Ficus benghalensis fruit extract (FE) with potassium silicate (K2SiO3) on productivity and biochemical composition of zucchini fruits, were assessed as biostimulants. The results showed that E. camaldulensis LE (4,000 mg/L) + K2SiO3 (500 mg/L) and T. viride (106 spore/ml) + K2SiO3 (500 mg/L) gave the highest significance yield of zucchini fruits. Furthermore, the total reading response of chlorophylls and carotenoids was significantly affected by biostimulants' treatments. The combination of K2SiO3 with E. camaldulensis LE increased the DPPH scavenging activity and the total phenolic content of zucchini fruits, in both experiments. However, the spraying with K2SiO3 did not observe any effects on the total flavonoid content of zucchini fruits. Several phenolic compounds were identified via high-performance liquid chromatography (HPLC) from the methanol extracts of zucchini fruits such as syringic acid, eugenol, caffeic acid, pyrogallol, gallic acid, ascorbic acid, ferulic acid, α-tocopherol, and ellagic acid. The main elemental content (C and O) analyzed via energy-dispersive X-ray spectroscopy (EDX) of leaves was affected by the application of biostimulants. The success of this work could lead to the development of cheap and easily available safe biostimulants for enhancing the productivity and biochemical of zucchini plants.
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