Biocompatible Patterning of Proteins on Wettability Gradient Surface by Thermo-Transfer Printing

Kim, S.; Ryu, Yong‐Sang; Suh, Jeng Hun; Keum, Chang‐Min; Sohn, Youngjoo; Lee, Sin Doo

doi:10.1166/jnn.2014.8828

Cited by 8 publications

(4 citation statements)

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“…Studies have shown that the transferred PDMS results in a large variation in the contact angle. 29) Based on the results of the AFM images, the capacitance-voltage characteristics, current-voltage characteristics, and contact angles of the bare and SCC-processed ferroelectric insulators, it was found that the surface morphology was modulated by the SCC process without varying the ferroelectric dielectric property and surface energy.…”

Section: Resultsmentioning

confidence: 99%

Surface engineering of ferroelectric polymer for the enhanced electrical performance of organic transistor memory

Kim

Lee

et al. 2018

Jpn. J. Appl. Phys.

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We suggest a viable surface control method to improve the electrical properties of organic nonvolatile memory transistors. For viable surface control, the surface of the ferroelectric insulator in the memory field-effect transistors was modified using a smooth-contact-curing process. For the modification of the ferroelectric polymer, during the curing of the ferroelectric insulators, the smooth surface of a soft elastomer contacts intimately with the ferroelectric surface. This smooth-contact-curing process reduced the surface roughness of the ferroelectric insulator without degrading its ferroelectric properties. The reduced roughness of the ferroelectric insulator increases the mobility of the organic field-effect transistor by approximately eight times, which results in a high memory on-off ratio and a low-voltage reading operation.

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Section: Resultsmentioning

confidence: 99%

Surface engineering of ferroelectric polymer for the enhanced electrical performance of organic transistor memory

Kim

Lee

et al. 2018

Jpn. J. Appl. Phys.

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“…In most previous works, the pattern roughness and the topographic mismatch across the hydrophobic and hydrophilic regions positioned underneath a supported lipid membrane were found to render lipid-free gaps 15 16 17 at monolayer-bilayer boundaries. In our case, the self-inking PDMS transfer 23 by thermally-assisted contact printing 24 25 ( Fig. 1B ) was employed to produce the MBJs with high spatial fidelity on a hydroxylated glass substrate.…”

Section: Resultsmentioning

confidence: 99%

“…Due to reactions of the transferred oligomers with silanols on the glass surface 27 28 , the hydrophobic patterns exhibited the long-term stability over 30 days 24 . As shown in Table 1 , the contact angle ( θ c ) of water on the S-PDMS surface increases monotonically with the printing temperature, i.e., the surface energy decreases.…”

Section: Resultsmentioning

confidence: 99%

“…The sandwiched substrate was heated on a hot plate at 200 °C for 3 min and the PDMS stamp was then removed. This thermally-assisted stamping process (at the elevated temperature of 200 °C) allowed the transfer of low-molecular weight PDMS oligomers from the PDMS stamp to the glass substrate 24 26 27 28 . A scanning electron microscope (SEM; XL30FEG, Philips) was used to observe the pattern structures of the PDMS stamp and an atomic force microscope (AFM; AutoProbe CP, Park Scientific) was employed for the surface topographic characterization.…”

Section: Methodsmentioning

confidence: 99%

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Continuity of Monolayer-Bilayer Junctions for Localization of Lipid Raft Microdomains in Model Membranes

Ryu

Wittenberg

Suh

et al. 2016

Sci Rep

Self Cite

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We show that the selective localization of cholesterol-rich domains and associated ganglioside receptors prefer to occur in the monolayer across continuous monolayer-bilayer junctions (MBJs) in supported lipid membranes. For the MBJs, glass substrates were patterned with poly(dimethylsiloxane) (PDMS) oligomers by thermally-assisted contact printing, leaving behind 3 nm-thick PDMS patterns. The hydrophobicity of the transferred PDMS patterns was precisely tuned by the stamping temperature. Lipid monolayers were formed on the PDMS patterned surface while lipid bilayers were on the bare glass surface. Due to the continuity of the lipid membranes over the MBJs, essentially free diffusion of lipids was allowed between the monolayer on the PDMS surface and the upper leaflet of the bilayer on the glass substrate. The preferential localization of sphingomyelin, ganglioside GM1 and cholesterol in the monolayer region enabled to develop raft microdomains through coarsening of nanorafts. Our methodology provides a simple and effective scheme of non-disruptive manipulation of the chemical landscape associated with lipid phase separations, which leads to more sophisticated applications in biosensors and as cell culture substrates.

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Elasticity‐Driven Membrane Budding through Cholesterol Concentration on Supported Lipid Monolayer–Bilayer Junction

Lee

Ahn

et al. 2020

Adv Materials Inter

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Membrane budding is an essential process during various biological activities, such as intercellular communication and transportation of life‐sustaining molecules and signals, which primarily occur with the aid of particular proteins. During such processes, the presence of cholesterol and its assembly into particular domains within the membranes have attracted considerable attention due to their unique characteristics in terms of the regulation of signal activities, membrane fluidity, and hormone production during metabolic pathways. However, despite these crucial roles, the precise mechanisms of their spatiotemporal localization toward specific areas and their physiological roles in membrane budding without the assistance of particular proteins remain unclear. Herein, a model membrane platform is reconstituted with lipid monolayer–bilayer junctions, which facilitate the selective concentration of cholesterol on the lipid monolayer regions. In vitro membrane budding is demonstrated by the remaining vesicles where the lipid monolayer membrane is ruptured. The physicochemical approach to the selective concentration of cholesterol and its consequent membrane vesiculation offer important clues to help understand the underlying mechanisms of cholesterol in the lipid‐driven membrane‐budding process.

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Biocompatible Patterning of Proteins on Wettability Gradient Surface by Thermo-Transfer Printing

Cited by 8 publications

References 0 publications

Surface engineering of ferroelectric polymer for the enhanced electrical performance of organic transistor memory

Surface engineering of ferroelectric polymer for the enhanced electrical performance of organic transistor memory

Continuity of Monolayer-Bilayer Junctions for Localization of Lipid Raft Microdomains in Model Membranes

Elasticity‐Driven Membrane Budding through Cholesterol Concentration on Supported Lipid Monolayer–Bilayer Junction

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