Biocompatible inorganic nanoparticles for [18F]-fluoride binding with applications in PET imaging

Abstract

A wide selection of insoluble nanoparticulate metal salts was screened for avid binding of [(18)F]-fluoride. Hydroxyapatite and aluminium hydroxide nanoparticles showed particularly avid and stable binding of [(18)F]-fluoride in various biological media. The in vivo behaviour of the [(18)F]-labelled hydroxyapatite and aluminium hydroxide particles was determined by PET-CT imaging in mice. [(18)F]-labelled hydroxyapatite was stable in circulation and when trapped in various tissues (lung embolisation, Subcutaneous and intramuscular), but accumulation in liver via reticuloendothelial clearance was followed by gradual degradation and release of [(18)F]-fluoride (over a period of 4 h) which accumulated in bone. [(18)F]-labelled aluminium hydroxide was also cleared to liver and spleen but degraded slightly even without liver uptake (Subcutaneous and intramuscular). Both materials have properties that are an attractive basis for the design of molecular targeted PET imaging agents labelled with (18)F.

Synthesis, Characterization, and Application of Core–Shell Co0.16Fe2.84O4@NaYF4(Yb, Er) and Fe3O4@NaYF4(Yb, Tm) Nanoparticle as Trimodal (MRI, PET/SPECT, and Optical) Imaging Agents

“…These observations were consistent with the core/shell structure of the NPs since neither Co 0.16 Fe 2.84 O 4 nor Fe 3 O 4 alone showed significant binding to [ 18 F]-fluoride. 35 The labeling efficiency (% of radionuclide bound) was found to increase with the amount of NPs, which is consistent with previous observations. 35 The serum stability of Co 0.16 Fe 2.84 O 4 @NaYF 4 (Yb, Er)-BP-PEG NPs prelabeled with [ 18 F]-fluoride was determined by incubating the fluorinated particles in human serum for intervals of up to 2 h and measuring the fraction of activity remaining bound to NPs with a gamma counter after separating NPs from the supernatant by centrifugation in a NanoSep device with a cutoff size of 10K (Figure S7).…”

In Vivo Integrity and Biological Fate of Chelator-Free Zirconium-89-Labeled Mesoporous Silica Nanoparticles

“… 12 Successful synthesis of intrinsically radio-labeled nanoparticle lies in the strong interaction between rationally selected nanoparticles and radio-isotopes. 12 Interesting combinations, such as 64 Cu-labeled porphysome, 13 arsenic-72 ( 72 As, t 1/2 = 26 h), and germanium-69 ( 69 Ge, t 1/2 = 39.1 h)-labeled SPION, 14 , 15 [ 64 Cu]CuS nanoparticles, 16 18 F-labeled upconversion nanoparticles and hydroxyapatite, 17 , 18 etc ., have been reported over the last several years. Although still in the early stages, design and synthesis of intrinsically radio-labeled nanoparticles have shown an attractive potential in offering an easier, faster, more stable, and more specific radio-labeling technique for the next generation of nano-oncology.…”

Effective Targeting of Colon Cancer Cells with Piperine Natural Anticancer Prodrug Using Functionalized Clusters of Hydroxyapatite Nanoparticles

“…We speculate that the main reason for the increase in HAP size is due to the agglomeration effect and not Pip loading or coating. After injection of the particles in vivo they are distributed in different organs depending on the different routes of in vivo application: sub-cutaneous, intramuscular, and intravenous injection [60,61]. Therefore, for future in vivo studies, the selection of intra-muscular, intradermal, or subcutaneous depot will be needed in order to avoid the barrier made by the reticulo-endothelial system (RES).…”