Effective Targeting of Colon Cancer Cells with Piperine Natural Anticancer Prodrug Using Functionalized Clusters of Hydroxyapatite Nanoparticles

AbouAitah, Khaled; Stefanek, Agata; Higazy, Iman M.; Janczewska, Magdalena; Świderska-Środa, Anna; Chodara, Agnieszka; Wojnarowicz, Jacek; Szałaj, Urszula; Shahein, Samar A; Aboul‐Enein, Ahmed M.; Abou-Elella, Faten; Gierlotka, S.; Ciach, Tomasz; Łojkowski, Witold

doi:10.3390/pharmaceutics12010070

Cited by 32 publications

(25 citation statements)

References 83 publications

(118 reference statements)

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“…Further coating with GoHAP resulted in new peaks centered at 1025, 601 and 586 cm −1 , corresponding to the nanoHAP powder spectrum. These peaks are associated with the phosphate stretching of P–O at 1025 cm −1 and the bending of P–O–P at 601 and 586 cm −1 [ 73 , 74 ]. By adding the drug, several peaks corresponding to it can be seen in the PEEK-HAP spectra.…”

Section: Resultsmentioning

confidence: 99%

Drug-Releasing Antibacterial Coating Made from Nano-Hydroxyapatite Using the Sonocoating Method

et al. 2021

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Medical implant use is associated with a risk of infection caused by bacteria on their surface. Implants with a surface that has both bone growth-promoting properties and antibacterial properties are of interest in orthopedics. In the current study, we fabricated a bioactive coating of hydroxyapatite nanoparticles on polyether ether ketone (PEEK) using the sonocoating method. The sonocoating method creates a layer by immersing the object in a suspension of nanoparticles in water and applying a high-power ultrasound. We show that the simple layer fabrication method results in a well-adhering layer with a thickness of 219 nm to 764 nm. Dropping cefuroxime sodium salt (Cef) antibiotic on the coated substrate creates a layer with a drug release effect and antibacterial activity against Staphylococcus aureus. We achieved a concentration of up to 1 mg of drug per cm2 of the coated substrate. In drug release tests, an initial burst was observed within 24 h, accompanied by a linear stable release effect. The drug-loaded implants exhibited sufficient activity against S. aureus for 24 and 168 h. Thus, the simple method we present here produces a biocompatible coating that can be soaked with antibiotics for antibacterial properties and can be used for a range of medical implants.

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Section: Resultsmentioning

confidence: 99%

Drug-Releasing Antibacterial Coating Made from Nano-Hydroxyapatite Using the Sonocoating Method

et al. 2021

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“…Therefore, there is a need for novel formulations to improve the dissolution and solubility of PPN. A number of nanosized delivery systems have been reported for the anticancer activity of PPN, such as piperine with paclitaxel liposomes [ 15 ], apamycin and piperine polymeric nanoparticles [ 16 ], piperine micelles [ 17 ], PIP hydroxyapatite nanoparticles [ 18 ], curcumin and piperine emulsomes [ 19 ] and protopanaxadiol-piperine nanoparticles [ 20 ]. They have reported enhanced activity from individual PIP-loaded nanoformulations, as well as combined delivery with PIP with other anticancer drugs.…”

Section: Introductionmentioning

confidence: 99%

Formulation of Piperine–Chitosan-Coated Liposomes: Characterization and In Vitro Cytotoxic Evaluation

et al. 2021

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The present research work is designed to prepare and evaluate piperine liposomes and piperine–chitosan-coated liposomes for oral delivery. Piperine (PPN) is a water-insoluble bioactive compound used for different diseases. The prepared formulations were evaluated for physicochemical study, mucoadhesive study, permeation study and in vitro cytotoxic study using the MCF7 breast cancer cell line. Piperine-loaded liposomes (PLF) were prepared by the thin-film evaporation method. The selected liposomes were coated with chitosan (PLFC) by electrostatic deposition to enhance the mucoadhesive property and in vitro therapeutic efficacy. Based on the findings of the study, the prepared PPN liposomes (PLF3) and chitosan coated PPN liposomes (PLF3C1) showed a nanometric size range of 165.7 ± 7.4 to 243.4 ± 7.5, a narrow polydispersity index (>0.3) and zeta potential (−7.1 to 29.8 mV). The average encapsulation efficiency was found to be between 60 and 80% for all prepared formulations. The drug release and permeation study profile showed biphasic release behavior and enhanced PPN permeation. The in vitro antioxidant study results showed a comparable antioxidant activity with pure PPN. The anticancer study depicted that the cell viability assay of tested PLF3C2 has significantly (p < 0.001)) reduced the IC50 when compared with pure PPN. The study revealed that oral chitosan-coated liposomes are a promising delivery system for the PPN and can increase the therapeutic efficacy against the breast cancer cell line.

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“…Piperine Emulsome combined with Curcu Emulsome (Piperine Curcumin loaded emulsomes, respectively) induced cancer cell death in HCT116 colon cancer cells in a high potential range (Bolat et al, 2020). Piperine loaded into Hydroxyapatite nanoparticles which was functionalized with phosphonate groups, successfully inhibited the monolayer of HCT116 cells and a perfect nanoformulation against colon cancer was obtained (Abouaitah et al., 2020).…”

Section: Studies On Cancer With Spices—major Bioactive Compoundsmentioning

confidence: 99%

Curry versus cancer: Potential of some selected culinary spices against cancer with in vitro, in vivo, and human trials evidences

et al. 2020

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Spices are dietary agents with immense potential for cancer chemo-prevention. A wide variety of spices are extensively used as food flavoring agents which possess potent antioxidant, anti-inflammatory, and anticancer properties due to the presence of certain bio-active compounds in them. In vitro, in vivo studies and clinical trials of selected spices against various types of cancer are being specified in this review. Effect of certain putative dietary spices namely turmeric, clove, garlic, ginger, fennel, black cumin, cinnamon, pepper, saffron, rosemary, and chilli along with its role in cancer are being discussed. Literature search was conducted through PubMed, Google scholar, Science direct, and Scopus using the keywords "spice," "cancer," "natural medicine," "herbal compound," "bioactive compounds." About 4,000 published articles and 127 research papers were considered to grab the brief knowledge on spices and their anticancer potential on a predefined inclusion and exclusion criteria. Practical application: Historically, spices and herbs are known for its traditional flavor, odor, and medicinal properties. Intensified risk of chronic and pervasive clinical conditions and increased cost of advanced drug treatments have developed a keen interest among researchers to explore the miscellaneous properties of herbal spices. Cancer is one of the deleterious causes of mortality affecting a huge number of populations worldwide. Arrays of cancer treatments including surgery, chemotherapy, and radiation therapy are used to compromise the disease but effective only when the size of the tumor is small. So, an effective treatment need to be developed that produces less side effects and herbal spices are found to be the promising agents. In this review, we illustrate about different in vitro, in vivo, and clinical studies of wide range of culinary spices having antineoplastic potential.

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Effective Targeting of Colon Cancer Cells with Piperine Natural Anticancer Prodrug Using Functionalized Clusters of Hydroxyapatite Nanoparticles

Cited by 32 publications

References 83 publications

Drug-Releasing Antibacterial Coating Made from Nano-Hydroxyapatite Using the Sonocoating Method

Drug-Releasing Antibacterial Coating Made from Nano-Hydroxyapatite Using the Sonocoating Method

Formulation of Piperine–Chitosan-Coated Liposomes: Characterization and In Vitro Cytotoxic Evaluation

Curry versus cancer: Potential of some selected culinary spices against cancer with in vitro, in vivo, and human trials evidences

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