Biocompatibility of various hydoxyapatite scaffolds evaluated by proliferation of rat’s bone marrow mesenchymal stem cells: an &lt;em&gt;in vitro&lt;/em&gt; study

Kamal, Achmad Fauzi; Iskandriati, Diah; Dilogo, Ismail Hadisoebroto; Siregar, Nurjati Chairani; Hutagalung, Errol Untung; Susworo, R.; Yusuf, Achmad A.; Bachtiar, Adang

doi:10.13181/mji.v22i4.600

Cited by 16 publications

(14 citation statements)

References 15 publications

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“…Interestingly, a higher amount of HA in our scaffolds resulted in slightly toxicity to hAMSCs and negatively impacted cell proliferation. Similar observations were reported by Kamal et al for a nanoHA paste scaffold [49]. The authors reported changes in cell morphology and impairment of cell attachment.…”

Section: Discussionsupporting

confidence: 88%

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Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles

Morejón

Delgado

Ribeiro

et al. 2019

IJMS

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Ceramic materials mimic the mineral composition of native bone and feature osteoconductive properties; they are therefore used to regenerate bone tissue. Much research focuses on increasing the porosity and pore interconnectivity of ceramic scaffolds to increase osteoconductivity, cell migration and cell-cell interaction. We aimed to fabricate biocompatible 3D-scaffolds featuring macro- and microporous calcium phosphates with high pore interconnection. Nanoparticles of hydroxyapatite (HA) and calcium deficient hydroxyapatite (CDHA) were synthesized by wet chemical precipitation. Scaffolds were produced from them by the replication polymeric foam technique. Solid content and sintering temperature were varied. Nanoparticles and scaffolds were characterized regarding morphology, chemical and mineral composition, porosity and mechanical properties. Biocompatibility, cell attachment and distribution were evaluated in vitro with human adipose mesenchymal stem cells. Scaffolds with total porosity of 71%–87%, pores in the range of 280–550 µm and connectivity density up to 43 mm−3 were obtained. Smaller pore sizes were obtained at higher sintering temperature. High solid content resulted in a decrease of total porosity but increased interconnectivity. Scaffolds 50HA/50β-TCP featured superior interconnectivity and mechanical properties. They were bioactive and biocompatible. High HA solid content (40 wt.%) in the HA pure scaffolds was negative for cell viability and proliferation, while in the 50HA/50β-TCP composite scaffolds it resulted more biocompatible.

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Section: Discussionsupporting

confidence: 88%

“…A proliferation inhibition value of 40% was reported for those nanoHA scaffolds. Unfortunately, no HA solid content was described for the tested material [49].…”

Section: Discussionmentioning

confidence: 99%

Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles

Morejón

Delgado

Ribeiro

et al. 2019

IJMS

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“…The structural properties, dosage, and chemical composition of the particles lead to the toxicity of the nanoparticles on the biological entities. 42,43 In this study, the cytotoxicity of MT-MPHA prepared using SA were investigated. Fig.…”

Section: Cell Adhesion/proliferation/toxicity Test On Mt-hapmentioning

confidence: 99%

Tailoring the morphological features of sol–gel synthesized mesoporous hydroxyapatite using fatty acids as an organic modifier

et al. 2019

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“…According to Kholine's research, 2012 HA-Ca sulfate can be used as MSC delivery material and does not affect the survival of MSCs. HA-Ca sulfate has biocompatibility in stimulating the adhesion of cells in biomaterial [9], [10].…”

Section: Discussionmentioning

confidence: 99%

Combining Mesenchymal Stem Cell from Stromal Vascular Fraction with Scaffold (in silico, Biocompatibility, and Attachment Study)

Sananta¹,

Imadudin²,

Mustaqim³

et al. 2020

Open Access Maced J Med Sci

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AIM: The aim of this study is to evaluate the in silico, biocompatibility, and attachment of mesenchymal stem cell (MSC) from stromal vascular fraction (SVF) combined with scaffolds. METHODS: This research is true experimental study. In silico study using HEX version 8.0. Attachment of MSC from SVF to scaffolds was evaluated using electron microscope and measured. Biocompatibility was evaluated using viability and apoptosis of MSC from SVF after combined with MSC with scaffold. RESULTS: From in silico study, the strongest bound to scaffold is between MSC from SVF to hydroxyapatite (HA) in both receptor which was integrin alpha V to HA which need a total energy of −89.24 (J/Mol) and integrin beta 2 to HA which need a total energy of −177.8 (J/Mol). MSC from SVF cells is capable to combine with three types of bone substitution material. At HA-calcium sulfate administration, SVF cells had apoptosis of 30.27%, and viable cells were 69.73%. At the administration of bovine bone cancellous, SVF cells had apoptosis of 22.20% and viable cells of 77.80%. From microscope electron study, the best result of attachment was obtained from MSC to HA-calcium phosphate with an average value of 12.66 cluster cells counting per 100 μm2 scaffold material. CONCLUSION: From in silico study showed that MSC from SVF could attach to scaffold with stronger binding between integrin alpha V and integrin beta 2 to HA scaffold. Bovine bone cancellous has the best biocompatibility and attachment among other scaffold with the highest viability and lowest cell apoptosis. From microscope electron study, we can prove that MSC can make a cluster cell in those scaffolds with HA-calcium sulfate having the biggest cluster cell counting.

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Biocompatibility of various hydoxyapatite scaffolds evaluated by proliferation of rat’s bone marrow mesenchymal stem cells: an <em>in vitro</em> study

Cited by 16 publications

References 15 publications

Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles

Development, Characterization and In Vitro Biological Properties of Scaffolds Fabricated From Calcium Phosphate Nanoparticles

Tailoring the morphological features of sol–gel synthesized mesoporous hydroxyapatite using fatty acids as an organic modifier

Combining Mesenchymal Stem Cell from Stromal Vascular Fraction with Scaffold (in silico, Biocompatibility, and Attachment Study)

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