Combining Mesenchymal Stem Cell from Stromal Vascular Fraction with Scaffold (in silico, Biocompatibility, and Attachment Study)

Abstract

AIM: The aim of this study is to evaluate the in silico, biocompatibility, and attachment of mesenchymal stem cell (MSC) from stromal vascular fraction (SVF) combined with scaffolds.
 METHODS: This research is true experimental study. In silico study using HEX version 8.0. Attachment of MSC from SVF to scaffolds was evaluated using electron microscope and measured. Biocompatibility was evaluated using viability and apoptosis of MSC from SVF after combined with MSC with scaffold.
 RESULTS: From in silico study, the strongest bound to scaffold is between MSC from SVF to hydroxyapatite (HA) in both receptor which was integrin alpha V to HA which need a total energy of −89.24 (J/Mol) and integrin beta 2 to HA which need a total energy of −177.8 (J/Mol). MSC from SVF cells is capable to combine with three types of bone substitution material. At HA-calcium sulfate administration, SVF cells had apoptosis of 30.27%, and viable cells were 69.73%. At the administration of bovine bone cancellous, SVF cells had apoptosis of 22.20% and viable cells of 77.80%. From microscope electron study, the best result of attachment was obtained from MSC to HA-calcium phosphate with an average value of 12.66 cluster cells counting per 100 μm2 scaffold material.
 CONCLUSION: From in silico study showed that MSC from SVF could attach to scaffold with stronger binding between integrin alpha V and integrin beta 2 to HA scaffold. Bovine bone cancellous has the best biocompatibility and attachment among other scaffold with the highest viability and lowest cell apoptosis. From microscope electron study, we can prove that MSC can make a cluster cell in those scaffolds with HA-calcium sulfate having the biggest cluster cell counting.