Biocompatibility of Orthodontic Resins: &lt;i&gt;In vitro&lt;/i&gt; Evaluation of Monomer Leaching and Cytotoxic Effects

Uomo, Roberto; Spagnuolo, Gianrico; Nocca, Giuseppina; Galeotti, Angela; Michelotti, Ambrosina; Valletta, Rosa; D’Antò, Vincenzo

doi:10.3844/crdsp.2017.8.14

Cited by 1 publication

(1 citation statement)

References 24 publications

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“…Uomo et al (2017) determined effect of orthodontic resins (Eagle Spectrum, Grengloo and Transbond XT) on cell viability by Alamar Blue test and evaluated released monomer both before and after resin polymerization using HPLC. The results showed the role of polymerized resin in determining cytotoxic effect of orthodontic resins and suggested that differences in chemical composition of resin matrix appeared to be much more related to decrease in cell viability than amount of monomer released from orthodontic resins.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity evaluation of dental and orthodontic light‐cured composite resins

Bationo

Rouamba

Diarra

et al. 2020

Clinical & Exp Dental Res

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Introduction The aim of this study was to determine the cytotoxicity of light‐cured composite resins (Clearfil ES‐2, Clearfil ES Flow, Filtek Supreme XTE, Grengloo, Blugloo, Transbond XT, and Transbond LR) then to assess leachable components in contact with human gingival fibroblasts (GFs) and to quantity detected bisphenol A (BPA). Methods Light‐cured composite resin discs were immersed for 24 hours in gingival fibroblastic medium (n = 3 for each product) and in control medium (n = 2 for each product) contained in plate. Cytotoxicity of the products (n = 95) was determined by the measure of cell viability using MTT assay after reading the optical densities of the plates. The analysis of leachable components was done by gas phase chromatography and mass spectrometry (GC–MS) and detected BPA was quantified. The limit of quantification was 0.01 μg/mL. Statistical analyses were performed by using IBM SPSS Statistics 20 and Kruskal–Wallis and Mann–Whitney U‐tests were applied. Results Cell viabilities were between 85 and 90%. Many chemical compounds including triethylene glycol dimethacrylate (TEGDMA) and BPA were identified. The average concentrations were 0.67 μg/mL ± 0.84 in the control medium and 0.73 μg/mL ± 1.05 in the fibroblastic medium. Filtek Supreme XTE presented the highest concentration of BPA with 2.16 μg/mL ± 0.65 and Clearfil ES Flow presented the lowest with 0.25 μg/mL ± 0.35. No BPA was detected with Transbond XT and Transbond LR. Clearfil ES Flow, Filtek Supreme XTE, Grengloo and Transbond LR presented residual TEGDMA. Conclusions Light‐cured composite resins are slightly cytotoxic opposite GFs and release many components including BPA and TEGDMA. Clinical precautions should be taken to decrease the release of these monomers.

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Section: Discussionmentioning

confidence: 99%