Biocompatibility of Developing 3D-Printed Tubular Scaffold Coated with Nanofibers for Bone Applications

Abstract

3D printing with controlled microarchitectures has gained traction in a wide variety of fields, including bone tissue engineering, because it represents an exciting alternative for the synthesis of new scaffolds due to its rapid manufacturing process, high precision, cost-effectiveness, and ease of use. Thus, this study is aimed at evaluating the biocompatibility response of a 3D-printed tubular scaffold coated by a layer of 7% PLA nanofibers. The morphology, structure, and chemical composition of the 3D-printed tubular scaffold were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier Transform Infrared (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and surface property analysis by profilometry. The biocompatibility response of the scaffold was assessed by cell adhesion, proliferation, and cell-material interactions of human fetal osteoblasts. Our results showed that 3D printing allowed obtaining similar and reproducible structures and the biocompatibility assays showed that nanofiber coating of the surface of the 3D tubular scaffold promoted an improvement on cell attachment, proliferation, and the morphology of osteoblast cells when compared with a noncoated scaffold. In conclusion, the surface of the 3D-printed tubular scaffold could be improved by the deposition of a nanofiber layer to render a more mimetic and active topography with excellent cellular biocompatibility for bone tissue applications.

Synthesis of a novel nanocomposite containing chitosan as a three‐dimensional printed wound dressing technique: Emphasis on gene expression

“…The placement of these strands together creates a highly porous space for gel and Cu‐CDs loading which are used as nutrients for tissue regeneration. Compared to freez‐drying and electrospining methods, this method is more simple and has a higher speed and accuracy the and scaffolds with proper mechanical strength and porosity can be obtained using this method 37 . Figure 1: (a) surface morphology; (b and c) distribution of chemical composition respective for oxygen, carbon represents the PLA scaffold.…”

Exploration of Bioengineered Scaffolds Composed of Thermo-Responsive Polymers for Drug Delivery in Wound Healing

“…The obtained 3D objects are highly customized and represent a cost-efficient production. This technique is probably the most adequate for controlling and modifying the internal microarchitecture of scaffolds [ 103 , 104 , 105 ].…”

Nanotechnologies and Nanomaterials in 3D (Bio)printing toward Bone Regeneration

“…[ 46 ] As such, a nanofiber layer coated on the 3D construct rendering a nanotopography was utilized to improve cell distribution in the musculoskeletal interface. [ 47 ] In this study, a melt extrusion 3D‐printed tube was modified by depositing a PLA nanofiber layer. This biomimetic nanofiber coating helped in improving cell adhesion, growth, and the osteoblast morphology.…”