Biocompatibility and osteogenicity of degradable Ca-deficient hydroxyapatite scaffolds from calcium phosphate cement for bone tissue engineering

Guo, Han; Su, Jiacan; Wei, Jie; Kong, Hang; Liu, Changsheng

doi:10.1016/j.actbio.2008.07.018

Cited by 161 publications

(98 citation statements)

References 35 publications

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“…Wang et al (2004a) compared the bone-related gene expression of human primary osteogenic sarcoma cell line SaOS-2 cultured on calcium phosphate ceramics with different phase compositions and different sintering temperatures in vitro. The gene expression patterns from his and other researches supported the biocompatibility and bioactivity potentials of calcium phosphate ceramics (Wang C. et al, 2004b;Wang H. et al, 2007;Wang J.J. et al, 2009;Guo et al, 2009). Previous research on safety was mainly focused on histological analysis of long-term tissue response after the biomaterials were implanted in the animals.…”

Section: Discussionmentioning

confidence: 99%

Osteoinduction by Ca-P biomaterials implanted into the muscles of mice

Yang

Cheng

et al. 2011

J. Zhejiang Univ. Sci. B

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Abstract:The osteoinduction of porous biphasic calcium phosphate ceramics (BCP) has been widely reported and documented, but little research has been performed on rodent animals, e.g., mice. In this study, we report osteoinduction in a mouse model. Thirty mice were divided into two groups. BCP materials (Sample A) and control ceramics (Sample B) were implanted into the leg muscle, respectively. Five mice in each group were killed at 15, 30, and 45 d after surgery. Sample A and Sample B were harvested and used for hematoxylin and eosin (HE) staining, immunohistochemistry (IHC) staining, and Alizarin Red S staining to check bone formation in the biomaterials. Histological analysis showed that no bone tissue was formed 15 d after implantation (0/5) in either of the two groups. Newly-formed bone tissues were observed in Sample A at 30 d (5/5) and 45 d (5/5) after implantation; the average amounts of newly-formed bone tissues were approximately 5.2% and 8.6%, respectively. However, we did not see any bone tissue in Sample B until 45 d after implantation. Bone-related molecular makers such as bone morphogenesis protein-2 (BMP-2), collagen type I, and osteopontin were detected by IHC staining in Sample A 30 d after implantation. In addition, the newly-formed bone was also confirmed by Alizarin Red S staining. Because this is the report of osteoinduction in the rodent animal on which all the biotechnologies were available, our results may contribute to further mechanism research.

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Section: Discussionmentioning

confidence: 99%

Osteoinduction by Ca-P biomaterials implanted into the muscles of mice

Yang

Cheng

et al. 2011

J. Zhejiang Univ. Sci. B

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“…The alternatives include use hierarchical bioactive scaffolds to engineer in vitro living cellular constructs for transplantation or use bioresorbable bioactive particulates or porous networks to activate in vivo the mechanisms of tissue regeneration [692,693]. Thus, the aim of calcium orthophosphate bioceramics is to prepare artificial porous scaffolds able to provide the physical and chemical cues to guide cell seeding, differentiation and assembly into 3D tissues of a newly formed bone [648,[694][695][696][697][698][699][700]. Particle sizes, shape and surface roughness of the scaffolds are known to affect cellular adhesion, proliferation and phenotype.…”

Section: Bioceramic Scaffolds From Calcium Orthophosphatesmentioning

confidence: 99%

Medical Application of Calcium Orthophosphate Bioceramics

Dorozhkin¹

2011

BIO

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In the late 1960's, a strong interest was raised in biomedical applications of ceramic materials (named bioceramics a little bit later). Bioceramics was initially used as reasonable alternatives to metals in order to increase their biocompatibility. However, within a short period, it has grown into a diverse class of biomaterials, presently including three essential types: relatively bioinert, bioactive (or surface reactive) and bioresorbable bioceramics. This review is limited to bioceramics prepared from calcium orthophosphates only, which belong to the categories of bioactive and bioresorbable compounds. There have been a number of important advances in this field during the past 30 -40 years. The research was shifted from an initial study on the develop-ment of bioinert bioceramics towards bioactive and bioresorbable bioceramics, and these different types of calcium orthophosphate bioceramics can be tuned through the structural and compositional control. At the turn of the millennium, a new concept of calcium orthophosphate bioceramics has been developed to promote a regeneration of bones. Current biomedical applications of calcium orthophosphate bioceramics include artificial replacements for hips, knees, teeth, tendons and ligaments, as well as repair for periodontal disease, maxillofacial reconstruction, augmenttation and stabilization of the jawbone, spinal fusion and bone fillers after tumor surgery. Potential future applications of calcium orthophosphate bioceramics are demonstrated in drug delivery systems, effective carriers of growth factors, bioactive peptides and/or various types of cells for tissue engineering purposes.

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“…Therefore, the specimen prepared was calcium-deficient hydroxyapatite [Ca 9.12 (HPO 4 Some studies have suggested that variation in the molar ratio of calcium to phosphate greatly affects the solubility of Ca-P biomaterials, and calcium phosphate with a Ca/P of 1.50 degraded faster than hydroxyapatite with a Ca/P of 1.67 when implanted in vivo. [7][8][9] Previous studies have shown that deficient calcium apatite, also called nonstoichiometric apatite, with a Ca/P of 1.50 was biologically more active than hydroxyapatite with a Ca/P of 1.67 because it has a composition and structure very close to that of natural bone mineral. 9 Thus, it is envisaged that n-CDHA can be fabricated as a novel bone regeneration material in order to get better bioperformance of apatite biomaterial.…”

Section: Morphology Of N-cdhamentioning

confidence: 99%

“…[6][7][8][9][10] Compared with nano hydroxyapatite, CDHA has higher solubility (ie, degradability) at a lower Ca/P ratio (1.5-1.67), as well as being more similar in composition and crystal structure to the mineral of natural bone. [7][8][9] Thus, n-CDHA is a better candidate as the inorganic phase of a degradable biocomposite.…”

Section: Introductionmentioning

confidence: 99%

Degradable biocomposite of nano calcium- deficient hydroxyapatite-multi(amino acid) copolymer

Hong

Gong²,

Yang³

et al. 2012

IJN

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Background and methods:A nano calcium-deficient hydroxyapatite (n-CDHA)-multi(amino acid) copolymer (MAC) composite bone substitute biomaterial was prepared using an in situ polymerization method. The composition, structure, and compressive strength of the composite was characterized, and the in vitro degradability in phosphate-buffered solution and preliminary cell responses to the composite were investigated. Results: The composite comprised n-CDHA and an amide linkage copolymer. The compressive strength of the composite was in the range of 88-129 MPa, varying with the amount of n-CDHA in the MAC (ranging from 10 wt% to 50 wt%). Weight loss from the composite increased (from 32.2 wt% to 44.3 wt%) with increasing n-CDHA content (from 10 wt% to 40 wt%) in the MAC after the composite was soaked in phosphate-buffered solution for 12 weeks. The pH of the soaking medium varied from 6.9 to 7.5. MG-63 cells with an osteogenic phenotype were well adhered and spread on the composite surface. Viability and differentiation increased with time, indicating that the composite had no negative effects on MG-63 cells. Conclusion:The n-CDHA-MAC composite had good cytocompatibility and has potential to be used as a bone substitute.

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Biocompatibility and osteogenicity of degradable Ca-deficient hydroxyapatite scaffolds from calcium phosphate cement for bone tissue engineering

Cited by 161 publications

References 35 publications

Osteoinduction by Ca-P biomaterials implanted into the muscles of mice

Osteoinduction by Ca-P biomaterials implanted into the muscles of mice

Medical Application of Calcium Orthophosphate Bioceramics

Degradable biocomposite of nano calcium- deficient hydroxyapatite-multi(amino acid) copolymer

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