Biochemical responses of the golden mussel Limnoperna fortunei under dietary glyphosate exposure

Iummato, María Mercedes; Sabatini, Sebastián E.; Cacciatore, Luis Claudio; Cochón, Adriana C.; Cataldo, Daniel; Molina, Marı́a del Carmen; Juárez, Ángela Beatriz

doi:10.1016/j.ecoenv.2018.07.046

Cited by 37 publications

(16 citation statements)

References 46 publications

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“…fortunei following exposure for 26 days to glyphosate (1, 3 and 6 mg/L) 42 . In a recent study conducted by the same authors on the same mussel species demonstrated that glyphosate did not affect significantly SOD and CAT activities after 28 days of dietary exposure with the microalgae Scenedesmus vacuolatus previously treated with a commercial formulation of glyphosate (6 mg/L, as active principle) 22 . The authors suggested that differences in the results of the two studies 22,42 could be due to exposure modality of mussels (glyphosate dose and availability in experiments with treated microalgae could be lower than those in waterborne exposure).…”

Section: Discussionmentioning

confidence: 90%

“…In a recent study conducted by the same authors on the same mussel species demonstrated that glyphosate did not affect significantly SOD and CAT activities after 28 days of dietary exposure with the microalgae Scenedesmus vacuolatus previously treated with a commercial formulation of glyphosate (6 mg/L, as active principle) 22 . The authors suggested that differences in the results of the two studies 22,42 could be due to exposure modality of mussels (glyphosate dose and availability in experiments with treated microalgae could be lower than those in waterborne exposure). In the clam Corbicula fluminea , exposure for 96 h to 2 and 10 mg/L of Roundup® caused a significant increase in SOD activity in gills and a significant decrease in CAT activity of digestive gland from animals treated with the highest concentration tested 21 .…”

Section: Discussionmentioning

confidence: 90%

“…Conversely, no significant alterations in AChE activity were recorded in L . fortunei following direct exposure to glyphosate 42 or via glyphosate-contaminated food (microalgae) 22 . Overall, results of our study suggested that the mixture of glyphosate and AMPA affected significantly AChE activity in mussels.…”

Section: Discussionmentioning

confidence: 99%

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Ecotoxicological hazard of a mixture of glyphosate and aminomethylphosphonic acid to the mussel Mytilus galloprovincialis (Lamarck 1819)

Matozzo

Munari

Masiero

et al. 2019

Sci Rep

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Assessment of the effects of chemical mixtures is a very important objective of the ecotoxicological risk assessment. This study was aimed at evaluating for the first time the effects of a mixture of glyphosate and its main breakdown product aminomethylphosphonic acid (AMPA) on various biomarkers in the mussel Mytilus galloprovincialis. Mussels were exposed for 7, 14 and 21 days to either 100 µg/L of glyphosate, 100 µg/L of AMPA or a mixture of both (100 + 100 µg/L). Various haemocyte parameters, such as total haemocyte counts, haemocyte diameter and volume, haemocyte proliferation, haemolymph lactate dehydrogenase activity and haemocyte lysate acid phosphatase activities were measured. In addition, the effects of exposure on the activity of antioxidant enzymes, acetylcholinesterase and glutathione-S-transferase were evaluated in gills and digestive gland from mussels. On the whole, this study demonstrated that the variables considered in the experimental plan, namely treatment, exposure time and their interaction, affect significantly biomarker responses in M. galloprovincialis. The effects of the mixture were comparable to those of the individual compounds, whereas their synergistic effects were occasionally observed, under the experimental conditions tested at least.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 90%

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Ecotoxicological hazard of a mixture of glyphosate and aminomethylphosphonic acid to the mussel Mytilus galloprovincialis (Lamarck 1819)

Matozzo

Munari

Masiero

et al. 2019

Sci Rep

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“…Imidacloprid-based neonicotinoids represent the greatest potential risk for non-target organisms living in local aquatic ecosystems such as dam lakes and rivers. Filter-feeding aquatic organisms may be exposed to these pesticides directly due to their contaminated environment or exposed by consuming contaminated microorganisms which are important nutrient sources [2].…”

Section: Resultsmentioning

confidence: 99%

Alterations in Some Biochemical Responses of Freshwater Mussels in Acute Imidacloprid Exposure

Yoloğlu¹

2019

Adıyaman University Journal of Science

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Imidacloprid is a widely used systemic neonicotinoid insecticide. The aim of this study is to identify potential harmful effects of imidacloprid in freshwater mussels (Unio mancus) which were exposed to sub-lethal concentrations (1, 10, 100, and 1000 µg AI L-1) for 96 h. For this aim, glutathione s-transferase (GST) activity, carboxylesterase (CaE) activity, acetylcholinesterase (AChE) activity, glutathione reductase (GR) activity, reduced glutathione (GSH) level, and malondialdehyde (MDA) level were evaluated as biochemical markers of exposure in the gills and digestive glands. The actual imidacloprid concentrations in the test waters were determined by LC-MS/MS analysis. The results showed that the AChE, GR, and CaE activities in the digestive glands significantly decreased at different exposure concentrations, while the MDA level significantly increased at the highest exposure concentration (p<0.05). Considering the results in the gills, the lowest exposure concentration caused inhibition in AChE activity, while the highest exposure concentration caused significant induction in GST activity, GSH level and MDA level (p<0.05). In addition, the measured imidacloprid concentrations were determined to be about 80% of the nominal imidacloprid concentrations. The data obtained from this study indicated that acute imidacloprid exposure caused biochemical alterations related to oxidative stress in mussels.

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“…Bivalves which are commonly used as biomonitor organisms in ecotoxicological studies, play significant roles in organic matter recycling and control of phytoplankton levels (Iummato et al, 2018). Bivalves, especially mussels, have various characteristics such as a wide geographic distribution, sessile life, deep burrowing, accumulation of many types of pollutants, and a high tolerance for chemical pollution (Okay et al, 2016;Burgos-Aceves, & Faggio, 2017;Guidi et al, 2017;Savorelli et al, 2017;Sureda, Capó, Busquets-Cortés, & Tejada, 2018).…”

Section: Introductionmentioning

confidence: 99%

Assesment of Na+/K+-ATPase, Mg2+-ATPase, Ca2+-ATPase, and Total-ATPase Activities in Gills of Freshwater Mussels Exposed to Penconazole

Yoloğlu

2019

Commagene Journal of Biology

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Penconazole, a triazole fungicide, is widely used worldwide to increase agricultural production and to control fungus. Bivalves, especially mussels, are specific biomonitor organisms of pollution in aquatic ecosystems. This study deals with the possible toxic effects of exposure to penconazole on Na + /K + -ATPase, Mg 2+ -ATPase, Ca 2+ -ATPase, and Total-ATPase activities in the gills of freshwater mussels (Unio mancus). In this context, freshwater mussels were exposed to different concentrations of penconazole (1, 10, 100, and 1000 µg AI L -1 ) in 96 hours static-renewal test system. The commercial form of penconazole (TOPAS ® 100 EC, Syngenta, Germany) was used in toxicity tests. The actual penconazole concentrations in the exposure media were determined by LC-MS/MS analysis. According to the LC-MS/MS analysis results, the actual penconazole concentrations in the exposure media were determined to be approximately 25% lower than the nominal penconazole concentrations. After 96 hours of exposure, the highest concentration of penconazole caused significant inhibition of ATPase activities except Mg 2+ -ATPase activity (P < 0.05). The observed data indicated that acute penconazole exposure may be a potential risk for freshwater mussels and that ATPase enzymes are important and useful biochemical markers for aquatic toxicology studies.Öz: Triazol bir fungusit olan penkonazol, tarımsal üretimi arttırmak ve fungusları kontrol etmek için dünya çapında yaygın olarak kullanılmaktadır. Çift kabuklu yumuşakçalar, özellikle midyeler, sucul ekosistemlerde kirliliğin spesifik biyomonitor organizmalarıdır. Bu çalışmada tatlı su midyelerinin (Unio mancus) solungaç Na + /K + -ATPaz, Mg 2+ -ATPaz, Ca 2+ -ATPaz ve Total-ATPaz aktiviteleri üzerine penkonazol maruziyetinin olası toksik etkileri değerlendirilmiştir. Bu amaçla, 96 saatlik statik yenileme test sisteminde tatlı su midyeleri farklı konsantrasyonlarda penkonazole (1, 10, 100 ve 1000 µg AI L -1 ) maruz bırakılmıştır. Toksisite testlerinde penkonazolün ticari formu (TOPAS ® 100 EC, Syngenta, Almanya) kullanılmıştır. Maruziyet ortamındaki gerçek penkonazol konsantrasyonları LC-MS/MS analizi ile belirlenmiştir. LC-MS/MS analizi sonuçlarına göre, maruziyet ortamındaki gerçek penkonazol konsantrasyonlarının, nominal penkonazol konsantrasyonlarından yaklaşık %25 oranında daha düşük olduğu belirlenmiştir. 96 saatlik maruziyetten sonra, en yüksek penkonazol konsantrasyonu Mg 2+ -ATPaz hariç diğer ATPaz aktivitelerinin önemli düzeyde inhibisyonuna neden olmuştur (P < 0.05). Elde edilen veriler, akut penconazol maruziyetinin tatlı su midyeleri için potansiyel bir risk olabileceğini ve ATPaz enzimlerinin akuatik toksikoloji çalışmaları için önemli ve faydalı biyokimyasal belirteçler olduğunu göstermiştir.Anahtar kelimeler: Penkonazol toksisitesi, tatlı su midyesi, ATPazlar, LC-MS/MS.

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Biochemical responses of the golden mussel Limnoperna fortunei under dietary glyphosate exposure

Cited by 37 publications

References 46 publications

Ecotoxicological hazard of a mixture of glyphosate and aminomethylphosphonic acid to the mussel Mytilus galloprovincialis (Lamarck 1819)

Ecotoxicological hazard of a mixture of glyphosate and aminomethylphosphonic acid to the mussel Mytilus galloprovincialis (Lamarck 1819)

Alterations in Some Biochemical Responses of Freshwater Mussels in Acute Imidacloprid Exposure

Assesment of Na+/K+-ATPase, Mg2+-ATPase, Ca2+-ATPase, and Total-ATPase Activities in Gills of Freshwater Mussels Exposed to Penconazole

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