Biochemical effect of resveratrol on Lipids profile and hepatic oxidative stress in experimentally induced obesity in female rats

Abstract

This study was designed to evaluate the effect of prolonged intake of resveratrol on lipid metabolism, liver enzymes and L-malondialdehyde(L-MDA) in obesity-induced in female rats by feeding high fat diet. Ninety female albino rats were divided into six equal groups of 15 rats each. Group Ι: rats fed normal control diet all over the periods of experiment (for 12 weeks). Group II: rats fed the control normal diet and administered resveratrol orally (0.1 mg/kg b.wt., orally) for 6 weeks. Group III: rats received the control normal diet and received resveratrol once daily (0.5 mg/kg b.wt., orally) for 6 weeks. Group IV: rats fed high fat diet (HFD) and received no drugs all over the periods of experiment (for 12 weeks). Group V: rats received HFD and administered resveratrol (0.1 mg/kg b.wt./day, orally) for 6 weeks. Group VI: rats fed HFD and administered resveratrol (0.5 mg/kg b.wt./day, orally). Blood samples were collected from all rats at 2, 4 and 6 weeks from the onset of resveratrol administration for determination of serum total cholesterol(TC), Triacylglycerols (TAG), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c), very low density lipoprotein cholesterol (VLDL-c), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and erythrocyte L-malondialdehyde (L-MDA).The obtained results showed, significant elevation of serum TC, TAG, LDL-c, VLDL-c, ALP,AST, ALT, L-MDA in obese rats as compared to rats fed normal control diet. While administration of resveratrol to HFD-fed rats tended to improve hepatic oxidative stress and dyslipidemia through improving total cholesterol and triacylglycerols and liver markers enzyme activities. These results suggest that, resveratrol is effective in improving the obesity and its associated many important complications such as diabetes mellitus and coronary heart disease and liver disease.

Cinnamon Reduces Dyslipidemia and Liver Steatosis Induced by High Fat Diet in Albino Rats: Histological, Ultrastructural, and Biochemical Studies

“…Although the serum TG increased significantly at the 4 th week, it decreased significantly after 8 weeks, in HFD-fed animals revealing the effect of time of HFD feeding on serum TG level. These results were in agreement with Hussein et al [26] who recorded a significant increase in serum levels of TC, TG, very low density lipoprotein, and LDL, while the level of HDL was significantly decreased in obese female rats. In addition, Venkateshan et al [27] attributed that to the oxidative stress of HFD that highly correlated with a wide variety of inflammatory and metabolic disease states including obesity.…”

Effects of Resveratrol Against Induced Metabolic Syndrome in Rats: Role of Oxidative Stress, Inflammation, and Insulin Resistance

“…Zhang et al [ 41 ] and Park et al [ 35 ] observed the same results in HFFD-fed rats. These disorders could be related to HFFD-mediated excessive delivery of fatty acids to the liver, resulting in the activation of oxidative stress and inflammatory pathways that promotes the escape of hepatic cellular enzymes [ 42 , 43 ].…”