Biochemical characterization of xylanase produced from Streptomyces sp. CS624 using an agro residue substrate

Mander, Poonam; Choi, Yun Hee; Pradeep, G C; Choi, Yun Seok; Hong, Joon Hee; Cho, Seung‐Sik; Yoo, Jin Cheol

doi:10.1016/j.procbio.2013.12.011

Cited by 35 publications

(16 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…CS428 (37 kDa) and Streptomyces sp. FA1 (43 kDa) showed greater larger molecular weight than r-XynS27 [ 11 , 12 , 29 , 30 ].…”

Section: Resultsmentioning

confidence: 99%

“…60% and 80% of the initial enzyme activity was retained at 60°C and 80°C, respectively. The optimal temperatures for the majority of previously described Streptomyces xylanases are between 60 and 70 °C [ 3 , 11 , 30 , 32 ]. However, Streptomyces sp.…”

Section: Resultsmentioning

confidence: 99%

“…FA1 (3.45 mg/mL), Streptomyces sp. CS624 (5.61 mg/mL) and Streptomyces thermovulgaris TISTR194 (0.76 mg/mL) [ 29 , 30 , 32 ]. However, comparisons between Km of xylanases are difficult since measurements rarely share the same conditions such as: incubation time, temperature, heating rate, protein concentration, and mainly substrate nature and concentration [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Improvement of bread making quality by supplementation with a recombinant xylanase produced by Pichia pastoris

et al. 2018

View full text Add to dashboard Cite

Xylanases (EC 3.2.1.8) are hydrolytic enzymes, which randomly cleave the β-1,4-linked xylose residues from xylan. The synthetic gene xynBS27 from Streptomyces sp. S27 was successfully cloned and expressed in Pichia pastoris. The full-length gene consists of 729 bp and encodes 243 amino acids including 51 residues of a putative signal peptide. This enzyme was purified in two steps and was shown to have a molecular weight of 20 kDa. The purified r-XynBS27 was active against beechwood xylan and oat spelt xylan as expected for GH 11 family. The optimum pH and temperature values for the enzyme were 6.0 and 75 °C, respectively. The Km and Vmax were 12.38 mg/mL and 13.68 μmol min/mg, respectively. The r-XynBS27 showed high xylose tolerance and was inhibited by some metal ions and by SDS. r-XynBS27 was employed as an additive in the bread making process. A decrease in firmness, stiffness and consistency, and improvements in specific volume and reducing sugar content were recorded.

show abstract

“…CS428 (37 kDa) and Streptomyces sp. FA1 (43 kDa) showed greater larger molecular weight than r-XynS27 [ 11 , 12 , 29 , 30 ].…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Improvement of bread making quality by supplementation with a recombinant xylanase produced by Pichia pastoris

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Given these considerations, the precipitation technique can be very effective in concentrating xylanase preparations, especially since high purity (which is typically achieved using chromatographic processes) may not be required for applications in areas such as the biofuels sector (Farinas et al, 2011). Although there are numerous reports concerning the isolation and purification of xylanases, most only describe the precipitation step for a single specific condition, usually for the purposes of biochemical characterization (Ahmad et al, 2013;Bokhari et al, 2009;Chanwicha et al, 2015;Lu et al, 2008;Mander et al, 2014;Ninawe et al, 2008).…”

Section: Brazilian Journal Of Chemical Engineeringmentioning

confidence: 99%

Ethanol Precipitation as a Downstream Processing Step for Concentration of Xylanases Produced by Submerged and Solid-State Fermentation

Costa

Farinas

Miranda

2018

Braz. J. Chem. Eng.

View full text Add to dashboard Cite

Xylanases have applications in different industries, being produced by microbial cultivations using submerged (SmF) or solid-state fermentation (SSF). Precipitation stands out as a potential method for the concentration of xylanases, especially with the use as ethanol as the precipitant due to its compatibility with the biorefinery concept. This paper presents a comparative laboratory scale study of ethanol precipitation of xylanases produced by Aspergillus niger cultivated under SSF and SmF. Precipitation conditions were selected according to a central composite design. Statistical analysis showed a significant effect of pH on the recoveries of total protein and xylanase activity. The kinetic profiles showed that a relatively short period of time (up to 15 min) was sufficient to recover most of the xylanase activity precipitated under the selected conditions. Xylanase recoveries of 65 and 79% were achieved for the SSF and SmF enzymatic complexes, respectively.

show abstract

“…These results were in accordance with xylanases isolated from Pleurotus ostreatus (Fathy et al, 2003), Clostridium thermocellum (Mandelli et al, 2014) and Streptomyces sp. CS624 (Mander et al, 2014). In the case of Bacillus sp.…”

Section: Xylooligosaccharides (Xos) Production From Birchwoodxylan Bymentioning

confidence: 99%

Production of antioxidant xylooligosaccharides from lignocellulosic materials using Bacillus amyloliquifaciens NRRL B-14393 xylanase

Rashad¹,

Mahmoud²,

Nooman³

et al. 2016

J App Pharm Sci

View full text Add to dashboard Cite

The present study aimed to evaluate the enzymatic production of XOS, antioxidant activities together with total phenolic contents from different lignocellulosic materials and birchwoodxylan using both crude and pure forms of Bacillus amyloliquifaciens NRRL B-14393 xylanase respectively. The mode of action of the pure xylanase was studied by HPLC and the end products analysis of birchwoodxylan revealed that xylose, xylobiose and xylotriose were the only end products. The resulted XOS mixture exhibited potent antioxidant activity of 87.36 % and high total phenolic content of 182.88 mg GAE/ liter of extract. XOS were obtained from different alkali pretreated agricultural residues by the crude xylanase action with a yield in a range from 54.4% to85.5%.Maximum scavenging abilities and total phenolic contents of XOS mixtures of all the tested materials were determined. XOS mixtures of orange and mango peels revealed relatively higher antioxidant activities (96% and 76.84%, respectively) and total phenolic acid contents (156.32 and 133.74 mg GAE/ liter of extract, respectively) compared to the other XOS mixtures. In conclusion, Bacillus amyloliquifaciens NRRL B-14393 xylanase could be a promising source for production of XOS with high phenolic contents and antioxidant activities.

show abstract

Biochemical characterization of xylanase produced from Streptomyces sp. CS624 using an agro residue substrate

Cited by 35 publications

References 22 publications

Improvement of bread making quality by supplementation with a recombinant xylanase produced by Pichia pastoris

Improvement of bread making quality by supplementation with a recombinant xylanase produced by Pichia pastoris

Ethanol Precipitation as a Downstream Processing Step for Concentration of Xylanases Produced by Submerged and Solid-State Fermentation

Production of antioxidant xylooligosaccharides from lignocellulosic materials using Bacillus amyloliquifaciens NRRL B-14393 xylanase

Contact Info

Product

Resources

About