Production of antioxidant xylooligosaccharides from lignocellulosic materials using Bacillus amyloliquifaciens NRRL B-14393 xylanase

Abstract

The present study aimed to evaluate the enzymatic production of XOS, antioxidant activities together with total phenolic contents from different lignocellulosic materials and birchwoodxylan using both crude and pure forms of Bacillus amyloliquifaciens NRRL B-14393 xylanase respectively. The mode of action of the pure xylanase was studied by HPLC and the end products analysis of birchwoodxylan revealed that xylose, xylobiose and xylotriose were the only end products. The resulted XOS mixture exhibited potent antioxidant activity of 87.36 % and high total phenolic content of 182.88 mg GAE/ liter of extract. XOS were obtained from different alkali pretreated agricultural residues by the crude xylanase action with a yield in a range from 54.4% to85.5%.Maximum scavenging abilities and total phenolic contents of XOS mixtures of all the tested materials were determined. XOS mixtures of orange and mango peels revealed relatively higher antioxidant activities (96% and 76.84%, respectively) and total phenolic acid contents (156.32 and 133.74 mg GAE/ liter of extract, respectively) compared to the other XOS mixtures. In conclusion, Bacillus amyloliquifaciens NRRL B-14393 xylanase could be a promising source for production of XOS with high phenolic contents and antioxidant activities.

Antioxidant activity of xylooligosaccharides produced from glucuronoxylan by Xyn10A and Xyn30D xylanases and eucalyptus autohydrolysates

“…Although the antioxidant capacity of XOS produced by xylanases has been reported in several works, to our knowledge, differences in the antioxidant effect of XOS obtained by different families of xylanases has not been described previously. The XOS described in literature come from different raw materials such as sugarcane bagasse (Bian et al, 2013;Mandelli et al, 2014), corncob (Gowdhaman & Ponnusami, 2015), garlic straw (Kallel, Driss, Chaabouni, & Ghorbel, 2014), wheat bran (Lasrado & Gudipati, 2014), wheat aleurone (Malunga & Beta, 2015), sunflower stalk and wheat straw (Akpinar, Erdogan, et al, 2010), or agricultural residues (Rashad et al, 2016 Rao & Muralikrishna (2006) showed that the presence of sugars with uronyl or acetyl groups impart strong antioxidant activity to cereal polysaccharides. It has been also reported that carboxyl groups increase antioxidant activity of cell wall polysaccharides (Pristov, Mitrovi, & Spasojevi, 2011).…”

“…In fact, high antioxidant activity has been reported in water-soluble arabinoxylans (feraxans) (Rao & Muralikrishna, 2006), isolated from rice and ragi. The phenolic content of oligosaccharides has been proposed to have an important effect in antioxidant activity (Bijalwan, Ali, Kesarwani, Yadav, & Mazumder, 2016;Rashad et al, 2016). On the other hand, in beechwood and birchwood XOS, since they do not contain ferulic acid, the main reason of their antioxidant capacity was the presence of methyl glucuronic acid ramifications.…”

Isolation and Identification of Hemicellulolytic Bacteria from Indonesian Coffee Pulp Waste

“…The supernatant contained crude enzymes of hemicellulase. Xylanase activity was measured using Nelson and Somogyi method [17,18]. One unit (U) of xylanase activity is defined as the number of enzymes needed to produce one milliliter of reducing sugars (xilose) per minute at 37 ºC.…”

Xylo-Oligosaccharides, Preparation and Application to Human and Animal Health: A Review

“…During both acute and chronic diseases in humans, the abundance of free radicals usually increases. Several notable studies demonstrated that XOS had exhibited strong antioxidant and free radical scavenging activity, thus suggesting a potential use in biomedical applications ( 52 , 53 ). The scavenging ability of XOS was shown to be dose-dependent ( 54 ), and this potential is likely attributable to efficient release of phenolic compounds and transfer of hydrogen atoms from the phenolic compounds to free radicals ( 55 ).…”