Biochemical characterization and purification of a binding protein for 24,25-dihydroxyvitamin D3 from chick intestine

Nemere, Ilka; Yazzie-Atkinson, D; Johns, DO; Larsson, D. G. Joakim

doi:10.1677/joe.0.1720211

Cited by 26 publications

(35 citation statements)

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“…The absence of a response to 1a,25(OH) 2 D 3 precludes the hypothesis that 1a,25(OH) 2 D 3 initiates a cascade leading to up-regulation of a 24R,25(OH) 2 D 3 -sensitive receptor. Moreover, the retention of the stimulatory effect of 24R,25(OH) 2 D 3 on PKC specific activity in the VDR(À/À) mice provides definitive evidence that at least some of the responses to this metabolite are not mediated through the 1,25-nVDR, as has also been suggested by others [de Boland and Norman, 1990;de Boland et al, 1994de Boland et al, , 1996Seo et al, 1996;Asada et al, 2001;Nemere et al, 2003].…”

Section: Discussionsupporting

confidence: 67%

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Membrane actions of vitamin D metabolites 1α,25(OH)₂D₃ and 24R,25(OH)₂D₃ are retained in growth plate cartilage cells from vitamin D receptor knockout mice

Boyan

Sylvia

McKinney

et al. 2003

J of Cellular Biochemistry

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1alpha,25(OH)(2)D(3) regulates rat growth plate chondrocytes via nuclear vitamin D receptor (1,25-nVDR) and membrane VDR (1,25-mVDR) mechanisms. To assess the relationship between the receptors, we examined the membrane response to 1alpha,25(OH)(2)D(3) in costochondral cartilage cells from wild type VDR(+/+) and VDR(-/-) mice, the latter lacking the 1,25-nVDR and exhibiting type II rickets and alopecia. Methods were developed for isolation and culture of cells from the resting zone (RC) and growth zone (GC, prehypertrophic and upper hypertrophic zones) of the costochondral cartilages from wild type and homozygous knockout mice. 1alpha,25(OH)(2)D(3) had no effect on [(3)H]-thymidine incorporation in VDR(-/-) GC cells, but it increased [(3)H]-thymidine incorporation in VDR(+/+) cells. Proteoglycan production was increased in cultures of both VDR(-/-) and VDR(+/+) cells, based on [(35)S]-sulfate incorporation. These effects were partially blocked by chelerythrine, which is a specific inhibitor of protein kinase C (PKC), indicating that PKC-signaling was involved. 1alpha,25(OH)(2)D(3) caused a 10-fold increase in PKC specific activity in VDR(-/-), and VDR(+/+) GC cells as early as 1 min, supporting this hypothesis. In contrast, 1alpha,25(OH)(2)D(3) had no effect on PKC activity in RC cells isolated from VDR(-/-) or VDR(+/+) mice and neither 1beta,25(OH)(2)D(3) nor 24R,25(OH)(2)D(3) affected PKC in GC cells from these mice. Phospholipase C (PLC) activity was also increased within 1 min in GC chondrocyte cultures treated with 1alpha,25(OH)(2)D(3). As noted previously for rat growth plate chondrocytes, 1alpha,25(OH)(2)D(3) mediated its increases in PKC and PLC activities in the VDR(-/-) GC cells through activation of phospholipase A(2) (PLA(2)). These responses to 1alpha,25(OH)(2)D(3) were blocked by antibodies to 1,25-MARRS, which is a [(3)H]-1,25(OH)(2)D(3) binding protein identified in chick enterocytes. 24R,25(OH)(2)D(3) regulated PKC in VDR(-/-) and VDR(+/+) RC cells. Wild type RC cells responded to 24R,25(OH)(2)D(3) with an increase in PKC, whereas treatment of RC cells from mice lacking a functional 1,25-nVDR caused a time-dependent decrease in PKC between 6 and 9 min. 24R,25(OH)(2)D(3) dependent PKC was mediated by phospholipase D, but not by PLC, as noted previously for rat RC cells treated with 24R,25(OH)(2)D(3). These results provide definitive evidence that there are two distinct receptors to 1alpha,25(OH)(2)D(3). 1alpha,25(OH)(2)D(3)-dependent regulation of DNA synthesis in GC cells requires the 1,25-nVDR, although other physiological responses to the vitamin D metabolite, such as proteoglycan sulfation, involve regulation via the 1,25-mVDR.

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Section: Discussionsupporting

confidence: 67%

“…The physicochemical properties of 1a,25(OH) 2 D 3 and 24R,25(OH) 2 D 3 are quite different [Seo et al, 1996;Kato et al, 1998], and they could interact with the same receptor in different ways. Arguing against this is report of a specific binding protein for 24R,25(OH) 2 D 3 isolated from chick lysosomal membranes [Nemere et al, 2003]. …”

Section: Discussionmentioning

confidence: 99%

Membrane actions of vitamin D metabolites 1α,25(OH)₂D₃ and 24R,25(OH)₂D₃ are retained in growth plate cartilage cells from vitamin D receptor knockout mice

Boyan

Sylvia

McKinney

et al. 2003

J of Cellular Biochemistry

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“…The ability of 24,25(OH) 2 D 3 to inhibit 1,25D 3 -MARRS receptor activation of protein kinase A and C activities was found in chick intestine [12] and kidney [4], which indicates the existence of a specific binding protein for 24,25(OH) 2 [10]. In order to explain how 24,25(OH) 2 D 3 works to effect inhibition, a cellular binding protein (66 kDa) was isolated, purified [10] and sequenced [13]. It was found to have a binding constant of 7 nM for 24,25(OH) 2 D 3 , and identified as catalase using Edman degradation techniques [13].…”

Section: Introductionmentioning

confidence: 92%

“…Thus, one possible mechanism for the inhibitory action would be the oxidation of thioredoxin domains in 1,25D 3 -MARRS receptor that occurred after 10 min of exposure [15], accompanied by a loss of binding activity. However, studies [15] showed a time-dependent decrease with either 24,25(OH) 2 In intestinal cells, studies showed 1,25D 3 -stimulated phosphate uptake is mediated by PKC signaling [3], and 24,25(OH) 2 D 3 seems to abolish that effect [10]. Thus, another possible mechanism would be the affecting the signal transduction pathway [17].…”

Section: Introductionmentioning

confidence: 99%

“…In chick intestinal cells, 1,25(OH) 2 D 3 has been found to have an acute, non-nuclear effect on phosphate transport, and 24,25(OH) 2 D 3 has been found to inhibit the reaction [8], with no effect on parathyroid hormone (PTH) stimulated phosphate transport [9]. In perfused chick duodenal loops, 24,25(OH) 2 D 3 inhibits the rapid stimulation of phosphate transport [8] mediated by 1,25(OH) 2 D 3 , as well as calcium transport [10].…”

Section: Introductionmentioning

confidence: 99%

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Effect of 24,25-dihydroxyvitamin D3 on localization of catalase in chick enterocytes

2015

Integr Mol Med

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The vitamin D metabolite, 24,25(OH) 2 showed punctuate staining of catalase inside the nucleus. Western analysis confirmed that the punctuate staining in the nucleus arose from the redistribution of cell surface catalase. Western analysis also indicated 24S,25(OH) 2 D 3 treatment resulted in redistribution of catalase to the nucleus, but to a lesser extent than treatment with 24R,25(OH) 2 D 3 . By understanding the molecular and cellular actions of 24,25(OH) 2 D 3 in chick intestine, progress will be made in enhancing phosphate and calcium absorption in animals to supply the minerals for adequate bone growth, and phosphate in manure of production animals could be diminished.

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ζ‐Chain associated protein 70 and CD38 combined predict the time to first treatment in patients with chronic lymphocytic leukemia

Giudice

Morilla

Osuji

et al. 2005

Cancer

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The autapomorphic status of the Neanderthal suprainiac fossa was recently confirmed. This was a result of a detailed analysis of the internal bone composition in the area of the suprainiac depression on Neanderthal and Homo sapiens specimens. However, while anatomical differences between Neanderthal suprainiac fossa and the depression in the inion region of the occipital bone of fossil and recent Homo sapiens have been discussed in detail, the etiology of these structures has not been resolved. In this article, the hypothesis that the Homo sapiens non‐supranuchal fossa and the Neanderthal suprainiac fossa both formed to maintain the optimal shape of the occipital plane (to minimize strain on the posterior cranial vault) is tested. First, the variation in the expression of the fossa above inion in the crania of recent Homo sapiens from European, African, and Australian samples was examined, and the degree of structural similarity between these depressions and the Neanderthal suprainiac fossa was assessed. Next, the relationship between the shape of the occipital squama in the midsagittal plane and two particular features (the degree of the occipital torus development and the occurrence of a depression in the inion region that is not the supranuchal fossa) were analyzed. Based on the results, it is suggested that the Homo sapiens non‐supranuchal fossa and Neanderthal suprainiac fossa are convergent traits. Am J Phys Anthropol, 2011. © 2010 Wiley‐Liss, Inc.

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Biochemical characterization and purification of a binding protein for 24,25-dihydroxyvitamin D3 from chick intestine

Abstract: An earlier study revealed that 24R,25-dihydroxyvitamin D 3 (24R,25(OH) 2 D 3 ) inhibits the rapid actions of 1,25(OH) 2 D 3 on stimulation of calcium transport in perfused duodena, as well as activation of protein kinases C and A. In the present work, a specific binding protein (24,

Cited by 26 publications

References 24 publications

Membrane actions of vitamin D metabolites 1α,25(OH)₂D₃ and 24R,25(OH)₂D₃ are retained in growth plate cartilage cells from vitamin D receptor knockout mice

Membrane actions of vitamin D metabolites 1α,25(OH)₂D₃ and 24R,25(OH)₂D₃ are retained in growth plate cartilage cells from vitamin D receptor knockout mice

Effect of 24,25-dihydroxyvitamin D3 on localization of catalase in chick enterocytes

ζ‐Chain associated protein 70 and CD38 combined predict the time to first treatment in patients with chronic lymphocytic leukemia

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Biochemical characterization and purification of a binding protein for 24,25-dihydroxyvitamin D3 from chick intestine

Abstract: An earlier study revealed that 24R,25-dihydroxyvitamin D 3 (24R,25(OH) 2 D 3 ) inhibits the rapid actions of 1,25(OH) 2 D 3 on stimulation of calcium transport in perfused duodena, as well as activation of protein kinases C and A. In the present work, a specific binding protein (24,

Cited by 26 publications

References 24 publications

Membrane actions of vitamin D metabolites 1α,25(OH)2D3 and 24R,25(OH)2D3 are retained in growth plate cartilage cells from vitamin D receptor knockout mice

Membrane actions of vitamin D metabolites 1α,25(OH)2D3 and 24R,25(OH)2D3 are retained in growth plate cartilage cells from vitamin D receptor knockout mice

Effect of 24,25-dihydroxyvitamin D3 on localization of catalase in chick enterocytes

ζ‐Chain associated protein 70 and CD38 combined predict the time to first treatment in patients with chronic lymphocytic leukemia

Contact Info

Product

Resources

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Membrane actions of vitamin D metabolites 1α,25(OH)₂D₃ and 24R,25(OH)₂D₃ are retained in growth plate cartilage cells from vitamin D receptor knockout mice

Membrane actions of vitamin D metabolites 1α,25(OH)₂D₃ and 24R,25(OH)₂D₃ are retained in growth plate cartilage cells from vitamin D receptor knockout mice