Effect of 24,25-dihydroxyvitamin D3 on localization of catalase in chick enterocytes

Abstract

The vitamin D metabolite, 24,25(OH) 2 showed punctuate staining of catalase inside the nucleus. Western analysis confirmed that the punctuate staining in the nucleus arose from the redistribution of cell surface catalase. Western analysis also indicated 24S,25(OH) 2 D 3 treatment resulted in redistribution of catalase to the nucleus, but to a lesser extent than treatment with 24R,25(OH) 2 D 3 . By understanding the molecular and cellular actions of 24,25(OH) 2 D 3 in chick intestine, progress will be made in enhancing phosphate and calcium absorption in animals to supply the minerals for adequate bone growth, and phosphate in manure of production animals could be diminished.