Biochemical characteristics of a novel ethanol-tolerant xylanase from Bacillus subtilis subsp. subtilis JJBS250 and its applicability in saccharification of rice straw

Biochemical Characterization of Cellulase From Bacillus subtilis Strain and its Effect on Digestibility and Structural Modifications of Lignocellulose Rich Biomass

“…Also, the effect of metal ions on the activity of enzymes seems to vary between different microorganisms (de Cassia Pereira et al, 2017). In this study, ZnSO 4 was found to accelerate the activity of the cellulase which correlates well with other studies that demonstrated the positive effect of ZnSO 4 on cellulase activity (Panthi et al, 2016;Sudip et al, 2020;Kumar and Singh, 2021). The stimulatory effect of Mg 2+ for the cellulase enzyme was also reported by Sudip et al (2020) for bacterial cellulase from B. subtilis Strain CBS31.…”

“…The detergent Triton X-100 does not react strongly with protein surfaces because it is considered a mild detergent. The same effect of Triton X-100 and SDS on enzyme activity was also reported by Kumar and Singh. (2021).…”

“…All the additives mentioned were separately mixed with the enzyme in 50 mM sodium citrate buffer at pH 5.0. The reaction mixtures were separately incubated at 37 °C for 30 min (Kumar and Singh, 2021). Then after the individual reactions were carried out with 1% of CMC, and relative activity was estimated by the DNS method.…”

“…Both bacteria and fungi are capable of secreting cellulolytic enzymes. Mostly the bacterial species have been used predominantly in industrial processes, wherein the Bacillus genus occupied a prime space for secreting a collection of hydrolytic enzymes (Kumar and Singh, 2021). Bacteria are prolific producers of cellulases as these enzymes are extracellularly secreted in abundant amounts, facilitating easy extraction and purification processes.…”

“…The bacteria screened from the environment harbor many properties like being thermophilic or psychrophilic, alkaliphilic, or acidophilic. This spacious diversity obtained by the microbes helps out in screening the efficient cellulase producing bacteria to overcome the present challenges associated with industrial processes (Rastogi et al, 2010;Irfan et al, 2017;Dehghanikhah et al, 2020;Kumar and Singh, 2021). Enzymes with high-temperature stability over extended periods have proved to be very efficient in industrial biomass processes and are better suited to potential enzyme recovery and recycling (Jin et al, 2020).…”