Adding value to rice straw waste for high-level xylanase production using a new isolate of Bacillus altitudinis RS3025

Ketsakhon, Punpaporn; Thammasittirong, Anon; Thammasittirong, Sutticha Na-Ranong

doi:10.1007/s12223-022-00998-x

Cited by 13 publications

(5 citation statements)

References 51 publications

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“…The maximum xylanase production was through sugarcane bagasse (472.03 U/mL) under SSF conditions. Accordingly, enhanced xylanase production employing multiple agricultural substrates, as well as high levels of enzyme production at lower substrate-to-moisture ratios, have previously been reported (Ketsakhon et al 2022 ; Kaur et al 2023 ). Sugarcane bagasse is an inexpensive byproduct with high xylan content; therefore, it is one of the most utilizable components of media for xylanase production (Alokika and Singh 2020 ; Moran-Aguilar et al 2021 ).…”

Section: Resultsmentioning

confidence: 92%

“…Afterward, xylanase productivity gradually decreased with increasing temperature (35–60 °C), with the lowest xylanase activity (89.80 U/mL) at 60 °C. According to the literature, the optimum temperature for xylanase production ranged between 30 and 60 °C, depending on the producing strain (Irfan et al 2016 ; Ketsakhon et al 2022 ). Furthermore, the bacterial growth was gradually increased with temperature and maximized at 40 °C (OD 600 3.091).…”

Section: Resultsmentioning

confidence: 99%

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Valorization of lignocellulosic wastes for sustainable xylanase production from locally isolated Bacillus subtilis exploited for xylooligosaccharides’ production with potential antimicrobial activity

El-Gendi,

Badawy,

Bakhiet

et al. 2023

Arch Microbiol

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The worldwide availability of lignocellulosic wastes represents a serious environmental challenge with potential opportunities. Xylanases are crucial in lignocellulosic bio-hydrolysis, but the low enzyme productivity and stability are still challenges. In the current study, Bacillus subtilis (coded ARSE2) revealed potent xylanase activity among other local isolates. The enzyme production optimization revealed that maximum enzyme production (490.58 U/mL) was achieved with 1% xylan, 1.4% peptone, and 5% NaCl at 30 °C and pH 9. Furthermore, several lignocellulosic wastes were exploited for sustainable xylanase production, where sugarcane bagasse (16%) under solid-state fermentation and woody sawdust (2%) under submerged fermentation supported the maximum enzyme titer of about 472.03 and 485.7 U/mL, respectively. The partially purified enzyme revealed two protein bands at 42 and 30 kDa. The partially purified enzyme revealed remarkable enzyme activity and stability at 50–60 °C and pH 8–9. The enzyme also revealed significant stability toward tween-80, urea, DTT, and EDTA with Vmax and Km values of 1481.5 U/mL and 0.187 mM, respectively. Additionally, the purified xylanase was applied for xylooligosaccharides production, which revealed significant antimicrobial activity toward Staphylococcus aureus with lower activity against Escherichia coli. Hence, the locally isolated Bacillus subtilis ARSE2 could fulfill the xylanase production requirements in terms of economic production at a high titer with promising enzyme characteristics. Additionally, the resultant xylooligosaccharides revealed a promising antimicrobial potential, which paves the way for other medical applications.

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Section: Resultsmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Valorization of lignocellulosic wastes for sustainable xylanase production from locally isolated Bacillus subtilis exploited for xylooligosaccharides’ production with potential antimicrobial activity

El-Gendi,

Badawy,

Bakhiet

et al. 2023

Arch Microbiol

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“…Even after being incubated at 70 °C for half an hour, 48% of the xylanase activity was observed showing that xylanase produced from Bacillus safensis MABS6 can withstand high temperatures. The majority of bacterial xylanases reach their peak levels of activity between the temperatures of 50 to 60℃ (Ketsakhon et al, 2023). The optimum temperature for Bacillus xylanases was also found to be 50 °C (Saleem et al, 2021).…”

Section: Discussionmentioning

confidence: 91%

“…Maximum enzyme synthesis during fermentation occurs at various times for different bacteria, depending on the kind of organism, its enzyme production pattern, culture circumstances, and genetic composition (Zikmanis et al, 2020). Depletion of nutrients or proteolysis may be to blame for the drop in xylanase production (Ketsakhon et al, 2023). In Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Xylanase Production from Bacillus safensis MABS6 using Sorghum Straw Substrate: Optimization, Characterization, and Biotechnological Applications

Hassan Al-Shawi

2023

Catrina: The International Journal of Environmental Sciences

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The objective of this study was to evaluate the synthesis of xylanase enzyme by Bacillus safensis MABS6 using sorghum straw as a substrate. A comprehensive investigation was conducted to optimize xylanase yield by examining various parameters such as sorghum concentration, inoculum size, culture age, pH, temperature, and agitation speed. Additionally, the impact of nutritional additives, metallic ions, organic solvents, and alkaline H 2 O 2 treatment on xylanase production and activity was explored. Experimental trials were performed with specific parameters, including 4% w/v sorghum concentration, 2% inoculum size, 12 hours of culture age, pH 7, 35°C, and 250 rpm. Further improvements involved the addition of nutritional additives such as gelatin, xylose, and potassium nitrate. The effects of initial conditions, various metallic ions (Ca 2+ , Mn 2+ , and Fe 3+ ), and organic solvents (2-methyl propanol, methanol, and ethanol) on xylanase activity were evaluated. Additionally, saccharification investigations with a 4% alkaline H2O2 treatment assessed the enzymatic hydrolysis of sorghum straw. Optimized conditions resulted in a significant increase, with xylanase production reaching 3.49%. The partially purified xylanase exhibited over 60% relative activity within a pH range of 6 to 9 and demonstrated more than 55% activity between 45°C to 65°C after 1 hour. Certain metallic ions and organic solvents further enhanced xylanase activity. Notably, the xylanase derived from Bacillus safensis MABS6, utilizing sorghum straw, showcased desirable characteristics such as heat stability, alkali-solvent stability, and absence of cellulase activity. Its potential as a biocatalyst makes it valuable for various biotechnological applications, particularly in the efficient enzymatic hydrolysis of sorghum straw.

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“…By tapping into these agricultural residues, the production costs can be minimized and the sustainable utilization of waste materials can be promoted, aligning with eco-friendly practices in enzyme production. A vast array of agricultural residues, such as sugarcane bagasse [25], rice straw [26], rice bran [27], wheat bran [28], coconut husk [29], corn stalk [30], and corncob [31], can be used as the carbon source or both carbon and nitrogen sources for microbial xylanase synthesis.…”

Section: Introductionmentioning

confidence: 99%

Optimization Production of an Endo-β-1,4-Xylanase from Streptomyces thermocarboxydus Using Wheat Bran as Sole Carbon Source

Tran,

Doan,

Dinh

et al. 2024

Recycling

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Xylanases, key enzymes for hydrolyzing xylan, have diverse industrial applications. The bioprocessing of agricultural byproducts to produce xylanase through fermentation approaches is gaining importance due to its significant potential to reduce enzyme production costs. In this work, the productivity of Streptomyces thermocarboxydus TKU045 xylanase was enhanced through liquid fermentation employing wheat bran as the sole carbon source. The maximum xylanase activity (25.314 ± 1.635 U/mL) was obtained using the following optima factors: 2% (w/v) wheat bran, 1.4% (w/v) KNO3, an initial pH of 9.8, an incubation temperature of 37.3 °C, and an incubation time of 2.2 days. Xylanase (Xyn_TKU045) of 43 kDa molecular weight was isolated from the culture supernatant and was biochemically characterized. Analysis through liquid chromatography with tandem mass spectrometry revealed a maximum amino acid identity of 19% with an endo-1,4-β-xylanase produced by Streptomyces lividans. Xyn_TKU045 exhibited optimal activity at pH 6, with remarkable stability within the pH range of 6.0 to 8.0. The enzyme demonstrated maximum efficiency at 60 °C and considerable stability at ≤70 °C. Mg2+, Mn2+, Ba2+, Ca2+, 2-mercaptoethanol, Tween 20, Tween 40, and Triton X-100 positively influenced Xyn_TKU045, while Zn2+, Fe2+, Fe3+, Cu2+, and sodium dodecyl sulfate exhibited adverse impact. The kinetic properties of Xyn_TKU045 were a Km of 0.628 mg/mL, a kcat of 75.075 s−1 and a kcat/Km of 119.617 mL mg−1s−1. Finally, Xyn_TKU045 could effectively catalyze birchwood xylan into xylotriose and xylobiose as the major products.

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Adding value to rice straw waste for high-level xylanase production using a new isolate of Bacillus altitudinis RS3025

Cited by 13 publications

References 51 publications

Valorization of lignocellulosic wastes for sustainable xylanase production from locally isolated Bacillus subtilis exploited for xylooligosaccharides’ production with potential antimicrobial activity

Valorization of lignocellulosic wastes for sustainable xylanase production from locally isolated Bacillus subtilis exploited for xylooligosaccharides’ production with potential antimicrobial activity

Enhanced Xylanase Production from Bacillus safensis MABS6 using Sorghum Straw Substrate: Optimization, Characterization, and Biotechnological Applications

Optimization Production of an Endo-β-1,4-Xylanase from Streptomyces thermocarboxydus Using Wheat Bran as Sole Carbon Source

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