Biochemical Characterization of Cellulase From Bacillus subtilis Strain and its Effect on Digestibility and Structural Modifications of Lignocellulose Rich Biomass

Malik, Waseem Ayoub; Javed, Saleem

doi:10.3389/fbioe.2021.800265

Cited by 47 publications

(30 citation statements)

References 67 publications

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“…Increased peak intensities in the amorphous and crystalline regions of 4-Cl-OPD modified HSA demonstrated the formation of complex structures formation compared to native HSA. As a result, XRD findings confirmed significant structural changes in HSA after 4-Cl-OPD modification ( Gong et al, 2011 ; Malik and Javed, 2021 ).…”

Section: Discussionmentioning

confidence: 54%

4-Chloro-1,2-phenylenediamine induced structural perturbation and genotoxic aggregation in human serum albumin

Warsi

Habib²,

Talha³

et al. 2022

Front. Chem.

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4-Chloro-1,2-phenylenediamine (4-Cl-OPD) is a halogenated aromatic diamine used as a precursor in permanent hair color production. Despite its well-documented mutagenic and carcinogenic effects in various in vitro and in vivo models, its role in fibrillar aggregate formation and their genotoxic effect in therapeutic proteins has received less attention. The significance of human serum albumin (HSA) arises from its involvement in bio-regulatory and transport processes. HSA misfolding and aggregation are responsible for some of the most frequent neurodegenerative disorders. We used various complementary approaches to track the formation of amyloid fibrils and their genotoxic effect. Molecular dynamics study demonstrated the complex stability. The impact of 4-Cl-OPD on the structural dynamics of HSA was confirmed by Raman spectroscopy, X-ray diffraction, HPLC and SDS-PAGE. Fibrilllar aggregates were investigated using Congo red assay, DLS, and SEM. The genotoxic nature of 4-Cl-OPD was confirmed using plasmid nicking assay and DAPI staining, which revealed DNA damage and cell apoptosis. 4-Cl-OPD provides a model system for studying fibrillar aggregation and their genotoxic potential in the current investigation. Future studies should investigate the inhibition of the aggregation/fibrillation process, which may yield valuable clinical insights.

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Section: Discussionmentioning

confidence: 54%

4-Chloro-1,2-phenylenediamine induced structural perturbation and genotoxic aggregation in human serum albumin

Warsi

Habib²,

Talha³

et al. 2022

Front. Chem.

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“…That's why the addition of EDTA inhibit the activity of enzymes and it suggested that cellulase enzyme activity is metal cofactor dependent (Elbanna et al 2015). The inhibitory impact of ionic surfactant SDS is attributed to the distortion in the protein active site by nonspecific interactions, causing conformational changes which result in protein unfolding and instability, the same finding was documented by (Malik and Javed 2021).…”

Section: Discussionmentioning

confidence: 77%

Characterization of thermo/halo stable cellulase produced from halophilic Virgibacillus salarius BM-02 using non-pretreated biomass

Yousef

Mawad

2022

World J Microbiol Biotechnol

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The production of extremozymes from halophilic bacteria has increased significantly due to their stability and efficiency in catalyzing a reaction, as well as their capacity to display optimum activity at various salt concentrations. In the current study, the halophilic bacterium Virgibacillus salarius strain BM-02 could utilize many non-pretreated substrates including cellulose, corn stover, sugarcane bagasse and wheat bran as a sole carbon source. However, wheat bran was the best substrate for achieving optimum saccharification yield (90.1%). The partially purified cellulase was active and stable at a wide range of pH (5–8) with residual activities > 58%. Moreover, it was stable at 5–12% of NaCl. Metal ions have a variable impact on the activity of partially purified cellulase however, Fe+3 exhibited the highest increase in the cellulase activity. The enzyme exhibited a thermal stability at 40, 50 and 60 °C with half-lives of 1049.50, 168.14 and 163.5 min, respectively. The value of Vmax was 22.27 U/mL while Km was 2.1 mM. The activation energy of denaturation Ed 69.81 kJ/mol, the enthalpy values (ΔHd) were positive, and the entropy values (ΔS) were negative. Therefore, V. Salarius is recommended as a novel promising halophilic extremozyme producer and agricultural waste remover in the bio-industrial applications. Graphical abstract

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“…The cellulase produced by NBCB1 remained active in a wide range of pH (3.5-9.5) and temperature (4-80 o C), which is a desirable characteristic for industrial applications [45]. The enzyme had pH and temperature optima of 5.…”

Section: Discussionmentioning

confidence: 99%

Valorization of Rice Straw for Optimization of Production of Thermotolerant Cellulase by Parageobacillus Thermoglucosidasius NBCB1 Under Submerged Fermentation

Basak

Murmu

Gavande

et al. 2022

Preprint

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Rice straw can be a substrate for production of cellulolytic enzymes, a vital step in economical deconstruction of cellulosic biomass in industry. We herein report the optimization of cellulase production by the thermotolerant Parageobacillus thermoglucosidasius NBCB1, in rice straw based submerged fermentation. Optimization of process parameters by One Factor At a Time (OFAT) suggested Day 3, medium pH 5.5, 1% (v/v) sorbitol, 1% (v/v) peptone and a cultivation temperature of 60°C as the optimal conditions for cellulase production. Further optimization of effective variables: incubation period, medium pH, sorbitol and peptone concentrations, by Response Surface Methodology (RSM) and Artificial Neural Networking (ANN) improved cellulase yield to 3.106 ± 0.005EU and 3.488 ± 0.004 EU/mg protein, respectively, which were closer to model diagnosed values. OFAT, RSM and ANN improved cellulase production by ~ 6.66, ~ 9.34 and ~ 10 folds, respectively, compared with enzyme production in unoptimized medium, indicating the later two being equally effective in the maximization of cellulase production. Purified cellulase (MW 31 kDa) with pH and temperature optima of 5.5 and 60°C, respectively, retained 100% activity upon pre-incubation at 60°C indicating it’s thermostability. It also showed halotolerance and heavy metal tolerance properties. Due to it’s versatile nature, the cellulase produced by P. thermoglucosidasius NBCB1 could be suitable for industrial degradation of agroresidues.

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Biochemical Characterization of Cellulase From Bacillus subtilis Strain and its Effect on Digestibility and Structural Modifications of Lignocellulose Rich Biomass

Cited by 47 publications

References 67 publications

4-Chloro-1,2-phenylenediamine induced structural perturbation and genotoxic aggregation in human serum albumin

4-Chloro-1,2-phenylenediamine induced structural perturbation and genotoxic aggregation in human serum albumin

Characterization of thermo/halo stable cellulase produced from halophilic Virgibacillus salarius BM-02 using non-pretreated biomass

Valorization of Rice Straw for Optimization of Production of Thermotolerant Cellulase by Parageobacillus Thermoglucosidasius NBCB1 Under Submerged Fermentation

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