Biochemical changes in tumor tissues of oral cancer patients

Kolanjiappan, K; Ramachandran, C. R.; Manoharan, Shanmugam

doi:10.1016/s0009-9120(02)00421-6

Cited by 109 publications

(87 citation statements)

References 22 publications

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“…Such results have been reported in breast cancer patients also [17]. Similar statistically significant MDA levels were observed in tissue samples which is in contrast to many studies [13,18,19] wherein the inverse relationship of TBARS to tumour progression was attributed to cellular proliferation [13], decreased concentration of free fatty acids In tumour tissue and decreased levels of phospholipids [18]. However, in the present study, the positive correlation between TBARS and tumour tissue as compared to normal mucosa could be attributed to greater cyclooxygenase activity resulting from inflammatory mediators involved in tumour progression [20].…”

Section: Discussionsupporting

confidence: 86%

Assessment of Lipid Peroxides in Multiple Biofluids of Leukoplakia and Oral Squamous Cell Carcinoma Patients- A Clinico- Biochemical Study

Ganesan

Kumar²

2014

JCDR

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Background: Oral pre cancer and oral cancer results in lipid peroxidation, and assessment of lipid peroxides in body fluids may give insights into the role of anti oxidants in its management. Aim:The study was conducted to discern the varying levels of lipid peroxides in saliva, serum and tissue in oral pre cancer and oral cancer and also various forms of tobacco usage with sex as an added parameter. Materials and Methods:The levels of lipid peroxides were measured in saliva, serum and tissue in a total of 50 patients, 20 belonging to control, and 30 study group in which 10 with oral leukoplakia and 20 with histologically proven oral squamous cell carcinoma (OSCC). The mean value of malondialdehyde (MDA) were also recorded in males and females among the patients with oral leukoplakia and OSCC. Among the study group patients, the levels of MDA were also recorded in habits of smoking and chewing tobacco. Statistical analysis used:Student's independent t-test, one way ANOVA, Tukey HSD procedure.Results: Significantly elevated levels of lipid peroxides were seen in saliva, serum and tissue in oral leukoplakia and OSCC when compared to control patients. Among the study group, there were statistically significant increased levels of MDA in OSCC when compared to oral leukoplakia. There was also increase in MDA level in patients with smoking and chewing, but the variations seen in males and females were not very significant. Conclusion:The results clearly indicate the increase in lipid peroxidation in oral pre cancer and oral cancer with no significant difference between gender groups. The role of saliva as a relatively risk free and reliable, easy to obtain biofuid for diagnostic purposes has been highlighted. Also, since the levels of antioxidants are drastically decreased in carcinogenesis, the importance of anti oxidant supplements in the early stages of the disease has also been elucidated.

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Section: Discussionsupporting

confidence: 86%

Assessment of Lipid Peroxides in Multiple Biofluids of Leukoplakia and Oral Squamous Cell Carcinoma Patients- A Clinico- Biochemical Study

Ganesan

Kumar²

2014

JCDR

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“…including prostate cancer and oral cancer [48,49], and dysregulated in lung and breast cancers [50,51]. Our data indicating that MDA-MB-231, that is highly aggressive tumor type representative, has an higher concentration of cholesterol in rafts and resistance to PUFA incorporation are in line to above cited reports.…”

supporting

confidence: 91%

Chemical–Physical Changes in Cell Membrane Microdomains of Breast Cancer Cells After Omega-3 PUFA Incorporation

Corsetto

Cremona

Montorfano

et al. 2012

Cell Biochem Biophys

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“…15 Some cancer tissues show increased cholesterol content compared to normal tissues, concomitant to enhanced antioxidant capacity, making them less susceptible to oxidative stress, thus conferring a selective growth advantage to tumor cells. 16 Here we show that S/M2 metastatic melanoma cells have higher cholesterol levels, higher DHCR24 gene expression and higher resistance to cytotoxic oxidative stress than S/P autologous tumor cells derived from the primary lesion. High levels of DHCR24 gene expression are detected by quantitative RT-PCR in 13/25 melanoma metastases, and in 1/7 primary melanomas, while melanocytes displayed low levels of expression.…”

Section: Discussionmentioning

confidence: 58%

DHCR24 gene expression is upregulated in melanoma metastases and associated to resistance to oxidative stress‐induced apoptosis

Stasi

Vallacchi

Campi

et al. 2005

Intl Journal of Cancer

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The DHCR24 gene encoding for the 3β‐hydroxysterol Δ24‐reductase, an oxidoreductase involved in cholesterol biosynthesis, was isolated by subtractive hybridization as highly expressed in a short‐term melanoma cell line derived from a cutaneous metastases (S/M2) compared to that obtained from the autologous primary tumor (S/P). DHCR24 (alias seladin‐1, diminuto/dwarf1 homolog) has been reported to act as an antiapoptotic factor in neurons. Gene expression analysis by Northern blot confirmed that DHCR24 was 5‐fold upregulated in S/M2 compared to S/P cells. High levels of DHCR24 gene expression were detected in 13/25 melanoma metastases and in 1/7 primary melanomas by real‐time PCR, indicating that upregulation of this gene may occur in melanoma progression. In S/M2 cells, high DHCR24 gene expression associated with resistance to apoptosis triggered by oxidative stress induced by exposure to hydrogen peroxide. DHCR24 gene transfer was shown to protect melanoma cells from H2O2‐induced cytotoxicity. Although higher cholesterol levels were shown in S/M2 cells compared to S/P cells, DHCR24 gene transfer did not increase cholesterol content. To evaluate whether DHCR24 acts as an antiapoptotic factor in melanoma metastases, the cytotoxic effect of chemotherapeutic agents was tested in DHCR24 transfectants and in the presence of a DHCR24 inhibitor, U18666A. High DHCR24 gene expression in transfectants did not result in a higher resistance to cytotoxic agents; treatment with U18666A was cytotoxic in S/P cells with a lower DHCR24 content and showed additive cytotoxic effect only when associated with H2O2 and not with cysplatin or etoposide, indicating that the DHCR24 protective effect is exerted through an oxidative stress‐specific mechanism. © 2005 Wiley‐Liss, Inc.

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Biochemical changes in tumor tissues of oral cancer patients

Cited by 109 publications

References 22 publications

Assessment of Lipid Peroxides in Multiple Biofluids of Leukoplakia and Oral Squamous Cell Carcinoma Patients- A Clinico- Biochemical Study

Assessment of Lipid Peroxides in Multiple Biofluids of Leukoplakia and Oral Squamous Cell Carcinoma Patients- A Clinico- Biochemical Study

Chemical–Physical Changes in Cell Membrane Microdomains of Breast Cancer Cells After Omega-3 PUFA Incorporation

DHCR24 gene expression is upregulated in melanoma metastases and associated to resistance to oxidative stress‐induced apoptosis

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