Chemical–Physical Changes in Cell Membrane Microdomains of Breast Cancer Cells After Omega-3 PUFA Incorporation

Corsetto, Paola Antonia; Cremona, Andrea; Montorfano, G.; Jovenitti, I.E.; Orsini, F.; Arosio, Paolo; Rizzo, Angela Maria

doi:10.1007/s12013-012-9365-y

Cited by 80 publications

(80 citation statements)

References 69 publications

(65 reference statements)

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“…Increases in EPA and DHA concentration of PL have been demonstrated to decrease cell proliferation and increase apoptosis, by changes in EGFR/AKT/NFkappaB cell survival pathway or by inducing CD95-mediated apoptosis [2][3][4]15]. EPA, but not DHA, increased with SO treatment in the current study, and this fatty acid has been demonstrated to inhibit human breast cancers both in vitro [1][2][3][4][49][50][51][52] and in vivo [7,50] and induce apoptosis [2,4,53]. DPA was increased with SO treatment by more than threefold in tumorigenic cells.…”

Section: Discussionsupporting

confidence: 49%

Stearidonic acid-enriched flax oil reduces the growth of human breast cancer in vitro and in vivo

Subedi

Newell

et al. 2014

Breast Cancer Res Treat

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The 20 and 22 carbon n-3 long-chain polyunsaturated fatty acids (LCPUFA) inhibit the growth of tumors in vitro and in animal models, but less is known about the 18 carbon n-3, stearidonic acid (SDA). This study aimed to establish and determine a mechanism for the anti-cancer activity of SDA-enriched oil (SO). SO (26 % of lipid) was produced by genetically engineering flax and used to treat human tumorigenic (MDA-MB-231, MCF-7) and non-tumorigenic (MCF-12A) breast cells. Nu/nu mice bearing MDA-MB-231 tumor were fed SO (SDA, 4 % of fat). Cell/tumor growth, phospholipid (PL) composition, apoptosis, CD95, and pro-apoptotic molecules were determined in SO-treated cells/tumors. Compared to a control lipid mixture, SO reduced (p < 0.05) the number of tumorigenic, but not MCF-12A cells, and resulted in higher concentration of most of the n-3 fatty acids in PL of all cells (p < 0.05). However, docosapentaenoic acid increased only in tumorigenic cells (p < 0.05). SO diet decreased tumor growth and resulted in more n-3 LCPUFA, including DPA and less arachidonic acid (AA) levels in major tumor PL (p < 0.05). Treatment of MDA-MB-231 cells/tumors with SO resulted in more apoptotic cells (in tumors) and in vivo and in vitro, more CD95+ positive cells and a higher expression of apoptotic molecules caspase-10, Bad, or Bid (p < 0.05). Supplementing SO alters total PL and PL classes by increasing membrane content of n-3 LCPUFA and lowering AA (in vivo), which is associated with increased CD95-mediated apoptosis, thereby suggesting a possible mechanism for reduce tumor survival.

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Section: Discussionsupporting

confidence: 49%

Stearidonic acid-enriched flax oil reduces the growth of human breast cancer in vitro and in vivo

Subedi

Newell

et al. 2014

Breast Cancer Res Treat

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“…In recent years, modulation of lipid rafts by omega-3 FAs is emerging as a new field to better understand the mechanism of action of PUFAs as chemoprevention agents in cancer therapy. In particular, EPA changes lipid composition in lipid rafts resulting in an inhibitory effect on proliferation of several cancer cell types [101,102]. A strategy to reinforce the efficacy of chemotherapeutic drugs is to increase their cellular uptake linking them to lipophilic carriers.…”

Section: Cancer Therapymentioning

confidence: 99%

Omega-3 polyunsaturated fatty acids and cancer: lessons learned from clinical trials

Nabavi

Bilotto

Russo

et al. 2015

Cancer Metastasis Rev

131

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Over the past decades, extensive studies have addressed the therapeutic effects of omega-3 polyunsaturated fatty acids (omega-3 FAs) against different human diseases such as cardiovascular and neurodegenerative diseases, cancer, etc. A growing body of scientific research shows the pharmacokinetic information and safety of these natural occurring substances. Moreover, during recent years, a plethora of studies has demonstrated that omega-3 FAs possess therapeutic role against certain types of cancer. It is also known that omega-3 FAs can improve efficacy and tolerability of chemotherapy. Previous reports showed that suppression of nuclear factor-κB, activation of AMPK/SIRT1, modulation of cyclooxygenase (COX) activity, and up-regulation of novel anti-inflammatory lipid mediators such as protectins, maresins, and resolvins, are the main mechanisms of antineoplastic effect of omega-3 FAs. In this review, we have collected the available clinical data on the therapeutic role of omega-3 FAs against breast cancer, colorectal cancer, leukemia, gastric cancer, pancreatic cancer, esophageal cancer, prostate cancer, lung cancer, head and neck cancer, as well as cancer cachexia. We also discussed the chemistry, dietary source, and bioavailability of omega-3 FAs, and the potential molecular mechanisms of anticancer and adverse effects.

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“…Lipids were extracted from cellular pellets and from sucrose gradient fractions with three different chloroform/ methanol mixtures 1:1, 1:2, 2:1 (v/v) and partitioned with chloroform/methanol/water, 47:48:1, v/v/v and then with water in according to Folch with minor modifications [34]. The organic phase, after partitioning was assayed for fatty acid composition, phospholipid, and neutral lipid content.…”

Section: Lipid Extractionmentioning

confidence: 99%

“…The chromatographic separation of phospholipid classes was carried out as previously described [34]. Chromatographic separation of neutral lipids was obtained by a ternary gradient according to Homan [35]: (A) consisted of isooctane-tetrahydrofuran (99:1, v/v), (B) acetone-dichlorometahne (2:1, v/v), and C) 2-propanol-water (7.5 mM acetic acid and 7.5 mM ethanolamine) (85:15, v/v).…”

Section: Analysis Of Different Phospholipid Classes and Neutral Lipidsmentioning

confidence: 99%

Changes in Lipid Composition During Manganese-Induced Apoptosis in PC12 Cells

et al. 2015

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Lipid composition of membranes is fundamental to modulate signaling pathways relying on lipid metabolites and/or membrane proteins, thus resulting in the regulation of important cell processes such as apoptosis. In this case, membrane remodeling is an early event important for the activation of signaling leading to cell death and removal of apoptotic cells. In the present study, we analyzed phospholipid, cholesterol and fatty acid content during apoptosis induced by manganese in PC12 cells. Lipid analysis of whole cells and detergent-resistant membranes was carried out by HPLC/GC. Results showed that apoptosis is associated with changes in lipid composition detectable in whole cell extracts, namely cholesterol, phosphatidylserine and phosphatidylethanolamine decreases. Noteworthy, phosphatidylserine level reduction was detectable before to the detection of apoptosis, in correlation with our previous study carried out by radioactive labelling. By contrast, phosphatidylserine and phosphatidylethanolamine changes were not detected in detergent resistant membranes, which instead showed an altered composition in phosphatidylinositol, phosphatidylcholine and sphingomyelin in apoptotic cells.

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Chemical–Physical Changes in Cell Membrane Microdomains of Breast Cancer Cells After Omega-3 PUFA Incorporation

Cited by 80 publications

References 69 publications

Stearidonic acid-enriched flax oil reduces the growth of human breast cancer in vitro and in vivo

Stearidonic acid-enriched flax oil reduces the growth of human breast cancer in vitro and in vivo

Omega-3 polyunsaturated fatty acids and cancer: lessons learned from clinical trials

Changes in Lipid Composition During Manganese-Induced Apoptosis in PC12 Cells

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