Biochemical Changes in Stored Whole Blood : An Observational Study in a Tertiary Hospital Blood Bank

Abstract

Several studies are going on to study the effects of transfusing stored RBCs on recipients. Several biochemical changes occur in stored blood, which may have some medical effects on the recipients. The biochemical changes may vary from donar to donar as well as from Blood bank to Blood bank. Also effects of transfused RBCs will vary from recipient to recipient. Lot of clinical trial should be taken before assessing the safety of stored RBCs. OBJECTIVESTo study the various biochemical changes occurring in a stored whole blood. MATERIAL AND METHODSAnalysis of biochemical changes occurring in stored whole blood in 20 voluntary donars was done in a Blood bank of a Tertiary Hospital. RESULTSOf the 6 biochemical parameters studied, only significant changes were seen in potassium and albumin after storage. There was a significant decrease in albumin concentration and steady increase in potassium values. CONCLUSIONCertain biochemical changes do occur in stored whole blood over a duration of storage. As the demand for blood is high due to various medical conditions, the treating consultant should be aware of the biochemical changes seen in stored whole blood.

The effect of storage period on the platelet levels on whole blood in the blood bank refrigerator

“…Certain chemicals such as histamine, lipids, and cytokines released by leukocytes during storage directly affect the physical function and metabolism of red blood cells. The length of storage of whole blood affects changes in serum albumin levels, this is also similar to a study conducted by Bhargava et al in 2016 in India (n=30, p<0.05) (Bhargava et al, 2016;Mane et al, 2015).…”

“…During storage, red blood cells undergo progressive structural and functional changes that can reduce their function and viability of red blood cells. This is corroborated by a study by Mane et al (2015), which stated that there was a significant decrease in serum albumin levels in whole blood stored in blood banks (n=20, p<0.05). The release of oxygen from red blood cells decreases during storage.…”

Biochemical Changes in the Stored Whole Blood Assessed at Periodic Intervals in Cpda-1 Anticoagulant Containing Blood Bags: A Study at the Blood Bank of Tertiary Care Hospital of Southern Rajasthan

“…Blood is that magic potion that gives life to another person. Although we have made tremendous discoveries and inventions in science, we cannot yet make this magic potion called blood, reiterating the fact that human blood has no substitute [1].…”

“…Blood is a body fluid composed of RBCs, WBCs, platelets, and plasma, performing distinct functions in the body. The primary role of RBCs is to transport blood gases, nutrients, hormones, and immune complexes [1,2]. WBCs provide immunity, platelets help prevent bleeding, and plasma provides different proteins and electrolytes.…”

“…Administration of prolonged preserved blood cells has been well-correlated with multiple studies suggesting a risk of severe complication or death in critically ill patients, patients undergoing cardiac surgery, and those having received a massive transfusion. As the time duration of whole blood storage containing CPDA-1 anticoagulant increases, the sodium/potassium pump gets immobilized, causing a decrease in intracellular potassium with an increase in cytoplasmic sodium levels, glucose levels decline, and acidosis occurs as a result of low pH levels [1,4]. The stored fragile erythrocytes eventually trigger the formation of hemoglobin-containing microparticles with release of cellfree hemoglobin, resulting in transfusion difficulties.…”