Biochemical and immunological characterization of the major structural proteins of feline immunodeficiency virus

Steinman, R M; Dombrowski, J; O’Connor, Thomas P.; Montelaro, Ronald C.; Tonelli, Quentin; Lawrence, K.; Seymour, C; Goodness, Joel; Pedersen, Neils C.; Andersen, Philip R.

doi:10.1099/0022-1317-71-3-701

Cited by 51 publications

(33 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MAbs 3B7 and 1Cll reacted strongly with a 24K protein and faintly labelled other bands at 50K, 15K and 10K. The relative size of these proteins is consistent with previously published data for FIV gag products (O'Connor et al, 1989;Olmsted et al, 1989;Egberink et al, 1990;Steinman et al, 1990). This pattern could be attributed to recognition of both precursor and mature forms of the same gag proteins.…”

Section: Discussionsupporting

confidence: 80%

“…Several proteins encoded by FIV gag, pol and env genes have been identified; the gag gene encodes a polypeptide precursor with an apparent Mr of 50K (Talbott et al, 1989;Egberink et al, 1990;Steinman et al, 1990). When this protein is cleaved by the protease encoded by the pol gene, the major core protein p24 and at least two more proteins, p15 and pl0, are produced (Egberink et al, 1990;Steinman et al, 1990). Quantitative measurement of FIV has been conducted by immunofluorescence or by detection of reverse transcrip-0000-9853 © 1991 SGM tase activity in tissue culture supernatants.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of two monoclonal antibodies against feline immunodeficiency virus gag gene products and their application in an assay to evaluate neutralizing antibody activity

Fevereiro

Roneker

Laufs

et al. 1991

Journal of General Virology

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Characterization of two monoclonal antibodies against feline immunodeficiency virus gag gene products and their application in an assay to evaluate neutralizing antibody activity

Fevereiro

Roneker

Laufs

et al. 1991

Journal of General Virology

View full text Add to dashboard Cite

“…The relative binding affinities of llama and alpaca IgG for protein G were visualized by Coomassie blue staining of a sodium dodecyl sulfate (SDS) polyacrylamide gel. 19 This procedure provided a qualitative assessment of the equivalence of llama and alpaca serum for these assays for all ELISAs.…”

Section: Methodsmentioning

confidence: 99%

Specificity of four Serologic Assays for Mycobacterium Avium ss Paratuberculosis in Llamas and Alpacas: A Single Herd Study

et al. 2000

View full text Add to dashboard Cite

Abstract. An investigation was conducted for Mycobacterium avium ss paratuberculosis infections in a research herd of llamas and alpacas. Herd culture-negative status was established over a 23-month period by screening any individuals with any signs compatible with paratuberculosis (n ϭ 1), high serology values (n ϭ 8), or other health and research related reasons (n ϭ 24). There were no M. avium ss paratuberculosis isolates from radiometric cultures of multiple tissue and fecal samples from these individuals and no known sources of exposure. Paratuberculosis is uncommon in North American llamas and alpacas: only 5 cases were identified after an extensive search of the Veterinary Medical Data Base, diagnostic laboratory records, publication databases, and personal communications. Therefore, serum samples from llamas (n ϭ 84) and alpacas (n ϭ 16) in the culture-negative herd were used to obtain preliminary estimates of test specificity for 3 enzyme-linked immunoassays (ELISAs) and an agar gel immunodiffusion (AGID) assay kit for detecting serum antibodies to M. avium ss paratuberculosis in South American camelids. The ELISAs were modifications of established bovine assays for antibody detection. With provisional cutoffs, ELISA-A had 52 false positives (specificity 48%), ELISA-B had 8 false positives (specificity 92%), ELISA-C had two false positives (specificity 98%), and the AGID had 0 false positives (specificity 100%). The range of ELISA values for culture-positive llamas and alpacas (n ϭ 10) from other herds overlapped the range of values for culture-negative llamas and alpacas. The accuracy of the ELISAs may be improved by using age-and sex-specific cutoffs because uninfected male llamas and alpacas that were older than 1 year had higher values for some tests. These tests can be used for either llamas or alpacas; the protein-G conjugate ELISA (ELISA-B) may be useful for multispecies applications. These assays are best used for rapid presumptive diagnoses of llamas and alpacas with diarrhea and weight loss and as a screening tool for herds known to be exposed to infection. All seropositive results should be confirmed with culture.Recent reports have demonstrated that paratuberculosis is a potential health risk for South American camelids. 2,13 An ongoing outbreak in Australian alpacas (Lama paca) 13 has illustrated that, as in bovine herds, 8 when paratuberculosis is introduced into a previously unexposed herd, much of the herd can become infected prior to any animals showing clinical signs of disease. North American llamas (Lama glama) have been reported with culture-confirmed infections. 2 Because llamas and alpacas constitute a significant industry worldwide and because the value of individual animals can be substantial, paratuberculosis could cause severe economic losses if the disease becomes Received for publication September 29, 1998. established in the industry. Because llama and alpaca importations and animal transfers among herds are important to the genetic and economic health of the llama and a...

show abstract

“…Recent studies revealed that FIV genome has three major open reading frames (ORFs), i.e., gag, pol, and env as seen in other lentiviruses 1,10,17]. Among the ORFs, gag encodes a putative precursor of p 50 which cleaves into three major core proteins (p24, p 15, and p 10) 1,16].…”

mentioning

confidence: 99%

Expression of feline immunodeficiency virusgag gene inEscherichia coli

Furuya

Hasegawa²,

Saitoh³

et al. 1992

Archives of Virology

View full text Add to dashboard Cite

The gag gene of a Japanese feline immunodeficiency virus (FIV) isolate, designated as FIV TM 2, was expressed in Escherichia coli as a fusion protein with TrpE. Using this expressed protein, an enzyme-linked immunosorbent assay was developed for detection of antibodies to FIV gag protein in feline sera. With serum samples from a cat experimentally infected with FIV, it was demonstrated that the period of seroconversion detected by this method corresponded to that by Western blotting.

show abstract

Biochemical and immunological characterization of the major structural proteins of feline immunodeficiency virus

Cited by 51 publications

References 36 publications

Characterization of two monoclonal antibodies against feline immunodeficiency virus gag gene products and their application in an assay to evaluate neutralizing antibody activity

Characterization of two monoclonal antibodies against feline immunodeficiency virus gag gene products and their application in an assay to evaluate neutralizing antibody activity

Specificity of four Serologic Assays for Mycobacterium Avium ss Paratuberculosis in Llamas and Alpacas: A Single Herd Study

Expression of feline immunodeficiency virusgag gene inEscherichia coli

Contact Info

Product

Resources

About