Characterization of two monoclonal antibodies against feline immunodeficiency virus gag gene products and their application in an assay to evaluate neutralizing antibody activity

Virus neutralization reveals antigenic variation among feline immunodeficiency virus isolates

“…could have occurred as early as the first or second week of life and in the presence of the high levels of colostral antibody, supporting the suggestion that VNA do not eliminate virus from infected cats (Fevereiro et al, 1991).…”

“…The presence of VNA to the Petaluma strain of FIV (FIV/PET) has been reported in cats naturally or experimentally infected with FIV (Fevereiro et al, 1991 ;Yamamoto et al, 1991 ;Tozzini et al, 1992). Since all of the sera tested neutralized the single virus isolate used in these studies (FIV/PET), it was suggested that there may be little antigenic difference between FIV strains.…”

“…The neutralization assay was similar to that described previously (Fevereiro et al, 1991). CrFK/ID10/R cells were usefll throughout.…”

“…A small proportion of isolates also replicates in the CrFK fibroblast line (Pedersen et al, 1987), This system has proved useful in studies of FIV neutralization because of its great sensitivity (Fevereiro et al, 1991;Tozzini et al, 1992). In the present study four strains of FIV, adapted for growth in CrFK cells, were used.…”

“…An exceptionally high VNA titre of 257000 was recorded at week 19. Since VNA do not appear before 5 to 6 weeks after experimental infection by inoculation (Fevereiro et al, 1991;Hosie & Jarrett, 1990), infection of kitten 5…”