Biochemical and Histopathological studies on female and male Wistar rats fed on genetically modified soybean meals (Roundup Ready)

Abstract

Background: Roundup Ready became the most cultivated genetically modified (GM) crop in the world. The aim of this study was to evaluate the effects of a diet containing GM soybean meal (RUR) on male and female Wistar rats. The body weights, food consumption, hematology, serum biochemistry, organ weight, and histopathological examination were compared between rats fed on GM soybean and those fed non-GM soybean after consumption of test diets for 18 weeks. Methods: Sixty weaned Wistar albino rats (30 females and 30 males) were randomly assigned to three groups of ten rats for each group per each sex. All rats have been fed either a diet containing 0% GM soybean meal (control), 50% GM soybean meal (T1), and 100% GM soybean (T2). The body weights, food consumption, hematology, serum biochemistry, organ weight, and histopathological examination were compared between rats fed GM soybean and those fed non-GM Soybean after consumption of test diets for 18 weeks. Results: Examined hematological parameters did not indicate a statistically significant difference between the GM soybean-fed groups (T1 and T2) and their respective controls, though the WBCs of the male of treated groups (T1 and T2), platelets count, and hemoglobin (HB) of the female of (T1) group were slightly elevated. The examined biochemical blood parameters showed that the level of triglycerides (TG) was statistically significantly higher in the female treated groups (T1 and T2) and lactate dehydrogenase (LDH) was significantly higher in treated groups compared to their respective controls. Elevated levels of alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatinine were observed in some of the treated groups. Histopathological examination of selected organs (liver, kidney, and pancreas) and testes or ovaries and female mammary gland revealed histological alterations among different treated groups (T1 and T2) with their respective controls. Conclusion: The obtained results indicate the necessity to revise the safety standards of consumption of diets containing GM soybean.

Antitoxic Effects of Curcumin against Obesity-Induced Multi-Organs’ Biochemical and Histopathological Abnormalities in an Animal Model

“…The present study showed significantly higher serum insulin and glucose levels in the HFD group compared to the control group. The present results coincide with several researchers who confirmed that oxidative stress and inflammation are the major components responsible for the pathogenesis of insulin resistance (IR) [ 45 – 47 ].…”

Assessment of biosafety and toxicity of hydrophilic gel for implantation in experimental in vitro and in vivo models

“…In the group of male rats administered with "Activegel" at a dose of 500 mg/kg we registered a signi cantly lower hemoglobin concentration (4.8%) and higher glucose concentration (31.6%) compared with the control. One should note that the above changes in the indices of general and biochemical blood analysis of of rats, although different from the control group, remained within normal range [19,21,23,24].…”

“…In the group of female rats injected with the test substance at a dose of 500 mg/kg a signi cantly lower glucose concentration and higher concentration of inorganic phosphorus (46.7%) was observed compared with the control. It is important to note that these altered biochemical parameters, according to the literature, were within normal range [21,24]. Thus, the in vivo toxicity of the new generation hydrophilic gel was evaluated using generally accepted approaches to clinical trials of the compounds and it was shown that this substance, according to the classi cation of the Environmental Protection Agency can be classi ed as hazard category IV, is not toxic.…”

Assessment of biosafety and toxicity of hydrophilic gel for implantation in experimental in vitro and in vivo models

“…In the group of male rats administered with “Activegel” at a dose of 500 mg/kg we registered a significantly lower hemoglobin concentration (4.8%) and higher glucose concentration (31.6%) compared with the control. One should note that the above changes in the indices of general and biochemical blood analysis of of rats, although different from the control group, remained within normal range [ 19 , 20 , 22 , 23 ].…”

“…In the group of female rats injected with the test substance at a dose of 500 mg/kg a significantly lower glucose concentration and higher concentration of inorganic phosphorus (46.7%) was observed compared with the control. It is important to note that these altered biochemical parameters, according to the literature, were within normal range [ 20 , 23 ].…”