Biochemical Alterations as Markers of Dengue Hemorrhagic Fever

Villar-Centeno, Luis Ángel; Díaz-Quijano, Fredi Alexander; Martínez-Vega, Ruth Aralí

doi:10.4269/ajtmh.2008.78.370

Cited by 67 publications

(73 citation statements)

References 22 publications

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“…By diverting certain lipids from their original fate, NS1 has the potential to interfere with the biogenesis of endogenous lipoprotein particles (51) or to deregulate the intracellular lipid-sensing machinery during entry into target cells (52,53). In line with our observations, several reports show that DENVinfected patients who developed DHF/DSS present a decrease of HDL and low-density lipoprotein content in plasma as well as altered levels of cholesterol and triglycerides (54)(55)(56). Moreover, the nutritional status of children seems to have an impact on the risk of developing fatal DHF/DSS (57, 58).…”

Section: Discussionsupporting

confidence: 79%

Secreted dengue virus nonstructural protein NS1 is an atypical barrel-shaped high-density lipoprotein

Gutsche

Coulibaly

Voss

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

252

263

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Dengue virus (DENV) causes the major arboviral disease of the tropics, characterized in its severe forms by signs of hemorrhage and plasma leakage. DENV encodes a nonstructural glycoprotein, NS1, that associates with intracellular membranes and the cell surface. NS1 is eventually secreted as a soluble hexamer from DENV-infected cells and circulates in the bloodstream of infected patients. Extracellular NS1 has been shown to modulate the complement system and to enhance DENV infection, yet its structure and function remain essentially unknown. By combining cryoelectron microscopy analysis with a characterization of NS1 amphipathic properties, we show that the secreted NS1 hexamer forms a lipoprotein particle with an open-barrel protein shell and a prominent central channel rich in lipids. Biochemical and NMR analyses of the NS1 lipid cargo reveal the presence of triglycerides, bound at an equimolar ratio to the NS1 protomer, as well as cholesteryl esters and phospholipids, a composition evocative of the plasma lipoproteins involved in vascular homeostasis. This study suggests that DENV NS1, by mimicking or hijacking lipid metabolic pathways, contributes to endothelium dysfunction, a key feature of severe dengue disease.arbovirus | dengue hemorrhagic fever | amphiphilic proteins | secretion

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Section: Discussionsupporting

confidence: 79%

Secreted dengue virus nonstructural protein NS1 is an atypical barrel-shaped high-density lipoprotein

Gutsche

Coulibaly

Voss

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

252

263

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“…En la literatura científica hay reportes de niveles elevados de este marcador asociados con síntomas característicos de gravedad (6-9). Durante la infección por el virus del dengue, se genera una afección del miocito de los músculos estriados, lo que podría desencadenar la liberación de LDH (9,19). Otra manifestación frecuente en el dengue es el compromiso hepático, el cual también podría estar asociado con el incremento en los niveles de LDH (20,21 (9).…”

Section: Análisis De Datosunclassified

“…Con la albúmina se confirman los resultados de anteriores estudios en los que se afirma que tener niveles por debajo de 4 mg/dl incrementa el riesgo de dengue grave (9,10). La hipoalbuminemia es un indicador de extravasación plasmática.…”

Section: Análisis De Datosunclassified

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Alteraciones bioquímicas como marcadores predictores de severidad en pacientes con fiebre por dengue

et al. 2012

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Contribución de los autores:Luis Villar-Centeno aportó el diseño del estudio, el análisis y la interpretación de los datos, la redacción y la revisión crítica del manuscrito. Anyela Lozano-Parra contribuyó en la recolección y la obtención de los resultados, la interpretación de los datos y la redacción del manuscrito. Doris Salgado-García brindó soporte en la redacción del manuscrito. Óscar F. Herrán contribuyó en el análisis y la interpretación de los datos y la redacción del manuscrito. ARTÍCULO ORIGINAL Biomédica 2013;33(Supl. Introducción. El dengue es la infección transmitida por mosquitos más importante en el mundo. Existe información de que las alteraciones bioquímicas pueden utilizarse como herramientas predictoras de gravedad del dengue. Objetivo. Evaluar las alteraciones bioquímicas como posibles marcadores predictores de gravedad del dengue. Materiales y métodos. Se llevó a cabo un estudio de casos y controles anidado en una cohorte. Se seleccionaron al azar 125 casos con dengue grave y 120 controles con dengue no grave para evaluar los niveles séricos de lactato-deshidrogenasa (LDH), creatina cinasa (CK), proteína C reactiva (PCR) y albúmina, en sueros obtenidos en las primeras horas de la enfermedad. Para evaluar el valor diagnóstico de cada biomarcador, se establecieron puntos de corte con una sensibilidad del 90 % en la detección de casos graves. Resultados. Se observó una asociación entre los niveles de PCR por debajo de 9,8 mg/l (OR=0,04; IC 95% =0,02-0,08; p=0,000), de LDH inferiores a 400 U/L (OR=0,49; IC 95% =0,24-1,02; p=0,053) y de albúmina menor de 4 mg/dl (OR=3,46; IC 95% =1,96-6,12; p=0,000), con la gravedad del dengue. En contraste, los niveles de la CK no mostraron asociación con la gravedad de la enfermedad. Conclusiones. Los hallazgos de nuestro estudio sugieren una asociación de los niveles de PCR, LDH y albúmina con la gravedad del dengue. Estas pruebas bioquímicas podrían ser utilizadas como herramientas predictoras del curso clínico de la infección.Palabras clave: dengue, índice de gravedad de la enfermedad, marcadores biológicos, predicción, bioquímica, humanos. doi: http://dx.doi.org/10.7705/biomedica.v33i0.732 Biochemical alterations as prediction markers for the severity of illness in dengue fever patientsIntroduction: Dengue is the most important mosquito-borne infection in the world. There is evidence supporting the use of biochemical alterations as prediction tools for severity of illness in dengue. Objective: To evaluate biochemical alterations as potential prediction markers for severity in dengue. Materials and methods: This was a case-control study nested in a cohort. We randomly selected 125 severe dengue cases and 120 controls with non-severe dengue for measuring LDH, CK, CRP and albumin serum levels using acute phase sera. To evaluate the predictive value for each biomarker, we established cut-off points with 90% sensitivity in detecting severe cases. Results: There was association among the CRP levels < 9.8 mg/L (OR=0.04; 95%CI=0.02-0.08; p=0.000), <400 U/L LDH l...

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“…Alternatively, plasma leakage can also be seen from laboratory tests for hypoalbuminemia. (10) Regrettably, in daily practice hematocrit baseline values are not readily available and calculation of the degree hematocrit elevation is difficult. Furthermore, baseline hematocrit level may also be affected by conditions such as anemia, which can lead to a misleading diagnosis and inadequate therapy.…”

Section: Introductionmentioning

confidence: 99%

Serum lactate as predictor and diagnostic biomarker of plasma leakage in adult dengue patients

Bur

Suwarto

Santoso

et al. 2016

UnivMed

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BACKGROUNDDengue fever (DF) and dengue hemorrhagic fever (DHF) are differentiated by the occurrence in DHF of plasma leakage into the interstitial space as shown by pleural and peritoneal effusion, hemoconcentration, and intravascular hypovolemia. Perfusion dysfunction causes anaerobic metabolism, which leads to increased serum lactate. This study was to determine serum lactate as prognostic predictor and diagnostic biomarker of plasma leakage in adult dengue patients.

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Biochemical Alterations as Markers of Dengue Hemorrhagic Fever

Cited by 67 publications

References 22 publications

Secreted dengue virus nonstructural protein NS1 is an atypical barrel-shaped high-density lipoprotein

Secreted dengue virus nonstructural protein NS1 is an atypical barrel-shaped high-density lipoprotein

Alteraciones bioquímicas como marcadores predictores de severidad en pacientes con fiebre por dengue

Serum lactate as predictor and diagnostic biomarker of plasma leakage in adult dengue patients

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