Biocatalyst CAL-B catalyzed synthesis of modified nucleosides: An overview

Kumar, Rajesh; Kumar, Vijay; Mathur, Divya; Kumar, Ram; Kumar, Ajit; Prasad, Ashok K.

doi:10.1080/00397911.2018.1554745

Cited by 9 publications

(5 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was possible to effectively isolate both free nucleosides 50a and 50b, as well as their N (3)-regio-isomers 51a and 51b, which resulted in yields of 89 and 94 percent, respectively. 23 Synthesis of the fluorinated benzimidazole ribosides 17a-c and 18a-c, respectively, was accomplished with the help of the related benzimidazoles 52a-c and 53a-c. Following treatment with sodium methoxide in methanol, the acetyl protecting groups that had been present on ribosides 54a-c and 55a-c have been successfully eliminated.…”

Section: Methodsmentioning

confidence: 99%

Study on Benzimidazole: A Comprehensive Review

-¹,

-²,

-³

et al. 2023

IJFMR

View full text Add to dashboard Cite

The chemical compound known as benzimidazole has been assigned to the category of heterocyclic aromatic in one of the potential classifications for it. This structure has a notable pharmacophore position in the scientific discipline of medicinal chemistry and enjoys a privileged status within that discipline. As a result of the fact that it possesses a wide range of beneficial therapeutic effects, such as those of antiulcerants, antihypertensives, analgesics, edicines, antivirals, antifungals, anticancer, and antihistamines, it serves a very important purpose. This is due to the fact that it has a very important purpose. The literature review reveals that benzimidazole derivatives are effective chemicals, and a wide range of reviews that are currently available on the market for organic chemistry and medical specialist studies have confirmed that the molecules of these compounds are helpful against a variety of different microorganisms. The importance of the methodology used in its synthesis has piqued the curiosity of synthetic organic chemists, who have shown an interest in the processes involved. As a direct result of this, we have made an effort in our evaluation of the gift to assemble information on the chemistry that lies behind a wide variety of substituted benzimidazole byproducts and the many medicinal applications of these substances.

show abstract

Section: Methodsmentioning

confidence: 99%

Study on Benzimidazole: A Comprehensive Review

-¹,

-²,

-³

et al. 2023

IJFMR

View full text Add to dashboard Cite

show abstract

“…Undoubtedly, CALB, an enzyme possessing 317 amino acids with a molecular weight of 33 kDa and a pI = 6, is the most used lipase in biotransformation [51,[108][109][110][111][112][113][114][115][116][117], especially as a commercially immobilized preparation (Novozym 435) in which the enzyme is immobilized onto a macroporous resin (Lewatit VP OC1600, poly-methyl methacrylate crosslinked with divinyl-benzene) [118]. However, CALA remains a very interesting enzyme, although CALA is not as frequently used as CALB in biotransformations for organic synthesis [91,117] it presents some very peculiar features which make it very attractive.…”

Section: Lipase a From Candida Antarctica (Cala)mentioning

confidence: 99%

Biotechnological relevance of the lipase A from Candida antarctica

et al. 2021

View full text Add to dashboard Cite

“…CALA (45 kDa, isoelectric point 7.5 and optimum pH 7.0) is an extremely thermostable protein (optimum temperature above 90 °C), whereas CALB has a lower stability (optimum temperature around 40 °C) [19,20]. CALA displays activity using large substrates (bulky alcohols, esters and tertiary alcohols) [21,22], and it is possible to accommodate sterically hindered substrates [23].…”

Section: Introductionmentioning

confidence: 99%

“…CALB (33 kDa, isoelectric point 6.0 and optimum pH 7.0) has a very small lid [24]. Both enzymes possess the classical serine hydrolases catalytic triad [20,25].…”

Section: Introductionmentioning

confidence: 99%

Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation

Monteiro

Neto

Fechine

et al. 2019

IJMS

View full text Add to dashboard Cite

The synthesis of ethyl butyrate catalyzed by lipases A (CALA) or B (CALB) from Candida antarctica immobilized onto magnetic nanoparticles (MNP), CALA-MNP and CALB-MNP, respectively, is hereby reported. MNPs were prepared by co-precipitation, functionalized with 3-aminopropyltriethoxysilane, activated with glutaraldehyde, and then used as support to immobilize either CALA or CALB (immobilization yield: 100 ± 1.2% and 57.6 ± 3.8%; biocatalysts activities: 198.3 ± 2.7 Up-NPB/g and 52.9 ± 1.7 Up-NPB/g for CALA-MNP and CALB-MNP, respectively). X-ray diffraction and Raman spectroscopy analysis indicated the production of a magnetic nanomaterial with a diameter of 13.0 nm, whereas Fourier-transform infrared spectroscopy indicated functionalization, activation and enzyme immobilization. To determine the optimum conditions for the synthesis, a four-variable Central Composite Design (CCD) (biocatalyst content, molar ratio, temperature and time) was performed. Under optimized conditions (1:1, 45 °C and 6 h), it was possible to achieve 99.2 ± 0.3% of conversion for CALA-MNP (10 mg) and 97.5 ± 0.8% for CALB-MNP (12.5 mg), which retained approximately 80% of their activity after 10 consecutive cycles of esterification. Under ultrasonic irradiation, similar conversions were achieved but at 4 h of incubation, demonstrating the efficiency of ultrasound technology in the enzymatic synthesis of esters.

show abstract

Biocatalyst CAL-B catalyzed synthesis of modified nucleosides: An overview

Cited by 9 publications

References 63 publications

Study on Benzimidazole: A Comprehensive Review

Study on Benzimidazole: A Comprehensive Review

Biotechnological relevance of the lipase A from Candida antarctica

Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation

Contact Info

Product

Resources

About