2019
DOI: 10.3390/ijms20225807
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Ethyl Butyrate Synthesis Catalyzed by Lipases A and B from Candida antarctica Immobilized onto Magnetic Nanoparticles. Improvement of Biocatalysts’ Performance under Ultrasonic Irradiation

Abstract: The synthesis of ethyl butyrate catalyzed by lipases A (CALA) or B (CALB) from Candida antarctica immobilized onto magnetic nanoparticles (MNP), CALA-MNP and CALB-MNP, respectively, is hereby reported. MNPs were prepared by co-precipitation, functionalized with 3-aminopropyltriethoxysilane, activated with glutaraldehyde, and then used as support to immobilize either CALA or CALB (immobilization yield: 100 ± 1.2% and 57.6 ± 3.8%; biocatalysts activities: 198.3 ± 2.7 Up-NPB/g and 52.9 ± 1.7 Up-NPB/g for CALA-MNP… Show more

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Cited by 68 publications
(33 citation statements)
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“…It has a significant role in food and flavor industries, and the demand is increasing nowadays 27 . To meet the growing demand for ‘natural’ ethyl butanoate, studies have gradually focused on enzymatic synthesis 28,29 . Using ethyl butanoate as an ester example, this review summarizes most of the works that have been done on ethyl butanoate and will help to gather information and find out the future scopes to improve the production of ethyl butanoate.…”
Section: Introductionmentioning
confidence: 99%
“…It has a significant role in food and flavor industries, and the demand is increasing nowadays 27 . To meet the growing demand for ‘natural’ ethyl butanoate, studies have gradually focused on enzymatic synthesis 28,29 . Using ethyl butanoate as an ester example, this review summarizes most of the works that have been done on ethyl butanoate and will help to gather information and find out the future scopes to improve the production of ethyl butanoate.…”
Section: Introductionmentioning
confidence: 99%
“…After that time, the biocatalyst was separated from the solution by magnetic decanting and washed with sodium phosphate buffer solution (25 mM and pH 7) until neutrality. To determine the amount of enzyme immobilized on the support, the initial and final concentration of RML in the supernatant of the immobilization suspension was measured ( Bezerra et al, 2017 ; Monteiro et al, 2019b ). The protein concentration was determined using the method described by Bradford (1976) and bovine serum albumin was used as a reference ( Bradford, 1976 ).…”
Section: Methodsmentioning
confidence: 99%
“…Then 10 mL of APTES was added to the mixture. The system remained for another hour in the ultrasonic bath and after that time, the functionalized nanoparticles were washed four times with ethanol and dried under vacuum (Monteiro et al, 2019b).…”
Section: Synthesis Of Iron Magnetic Nanoparticles (Fe 3 O 4 ) Functiomentioning
confidence: 99%
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“…For monitoring the percentage of ethyl esters formed by the reaction, the methodology described by [98] and [99] was used, the analytical method of determining the acid index, official methodology [100], was used.…”
Section: Analysis Of the Percentage Of Ethyl Estersmentioning
confidence: 99%