Bioautography detection in thin-layer chromatography

Choma, Irena; Grzelak, Edyta M.

doi:10.1016/j.chroma.2010.12.069

Cited by 248 publications

(130 citation statements)

References 88 publications

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“…The initial fractionation of the crude extract (570 mg) by size exclusion column chromatography on Sephadex LH-20 (3 × 115 cm; methanol as the eluent) resulted in seven fractions A-G, based on thin layer chromatographic (TLC) analysis. These fractions were tested for antimicrobial activity against MRSA and B. subtilis using a bioautographic TLC assay (Choma & Grzelak, 2011 The structure elucidation of the isolated compounds 1 and 2 was done using NMR and mass spectral data. 1 H, 13 C and 2D NMR spectral datasets in CDCl 3 were obtained using a Bruker AVANCE 600-MHz spectrometer with a 5 mm cryoprobe, while the electrospray ionisation mass spectral (ESIMS) data were obtained using Bruker Esquire-LC electrospray spectrometer.…”

Section: Isolation and Structure Elucidation Of The Bioactive Componentsmentioning

confidence: 99%

Antimicrobial constituents of Hypocrea virens, an endophyte of the mangrove-associate plant Premna serratifolia L.

Ratnaweera¹,

Silva²,

Wijesundera³

et al. 2016

J. Natn. Sci. Foundation Sri Lanka

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Emergence of multidrug-resistant pathogenic microorganisms has prompted a worldwide search for new antibiotics from various sources. Endophytic fungi from unique habitats are considered as potential sources of novel bioactive compounds. Sri Lankan mangrove ecosystem is such a distinctive and unexploited resource for the discovery of structurally diverse and biologically active metabolites including antimicrobials. Nine endophytic fungi were isolated from the leaves and twigs of Premna serratifolia L. from a mangrove habitat in the Negombo lagoon and the antimicrobial activities of their laboratory cultures were evaluated. The most promising antimicrobial activity was exhibited by the endophytic fungus Hypocrea virens. Bioassay guided fractionation of the organic extract of this fungus led to the isolation of two known metabolites; the antimicrobial epidithiodioxopiperazine, gliotoxin (1), and the closely related but less active bisdethiobis(methylthio)gliotxin (2). The chemical structures of the two compounds were determined by spectroscopy and confirmed by comparison of mass and nuclear magnetic resonance (NMR) spectral data with the reported values for these molecules. The minimum inhibitory concentration (MIC) values obtained for gliotoxin (1) in the current study are 0.13 µg mL -1 for Bacillus subtilis, 16 µg mL -1 for Staphylococcus aureus, 32 µg mL -1 for Methicillin Resistant Staphylococcus aureus (MRSA) and Escherichia coli, 64 µg mL -1 for Pseudomonas aeruginosa and Candida albicans fungus. This is the first study to report the isolation of endophytic fungi from P. serratifolia and their antimicrobial activities.

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Section: Isolation and Structure Elucidation Of The Bioactive Componentsmentioning

confidence: 99%

Antimicrobial constituents of Hypocrea virens, an endophyte of the mangrove-associate plant Premna serratifolia L.

Ratnaweera¹,

Silva²,

Wijesundera³

et al. 2016

J. Natn. Sci. Foundation Sri Lanka

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“…The antibacterial effect of separated chamomile components was evaluated in vitro with direct bioautography (DB) [17][18][19][20][21][22] …”

Section: Bioassaymentioning

confidence: 99%

“…Direct bioautography (DB) [17][18][19][20][21][22] can be used to visualise the activity of different matrix components separated by planar layer chromatography against cells in the adsorbent bed. Therefore, it is suitable for guiding the isolation of effective compounds e.g.…”

Section: Introductionmentioning

confidence: 99%

Application of direct bioautography and SPME-GC-MS for the study of antibacterial chamomile ingredients

Móricz

Szarka

Ott

et al. 2012

Journal of Planar Chromatography – Modern TLC

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“…The potential applications of bioautography are summerized in the publication written by Choma [9]. Direct bioautography belongs to a large group of antimicrobial screening methods and is based on paper or layer separations [10].…”

Section: Introductionmentioning

confidence: 99%

Detection of antibacterial activity of essential oil components by TLC-bioautography using luminescent bacteria

Horváth

Kocsis

Lemberkovics

et al. 2013

Journal of Planar Chromatography – Modern TLC

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Bioautography detection in thin-layer chromatography

Cited by 248 publications

References 88 publications

Antimicrobial constituents of Hypocrea virens, an endophyte of the mangrove-associate plant Premna serratifolia L.

Antimicrobial constituents of Hypocrea virens, an endophyte of the mangrove-associate plant Premna serratifolia L.

Application of direct bioautography and SPME-GC-MS for the study of antibacterial chamomile ingredients

Detection of antibacterial activity of essential oil components by TLC-bioautography using luminescent bacteria

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