Application of direct bioautography and SPME-GC-MS for the study of antibacterial chamomile ingredients

Móricz, Ágnes M.; Szarka, Szabolcs; Ott, Péter G.; Kertesy, Dóra; Héthelyi, Éva B.; Szöke, Éva; Tyihák, E.

doi:10.1556/jpc.25.2012.3.6

Cited by 15 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another luminescent test, using a genetically modified plant, pathogenic bacterium Pseudomonas syringae pv. maculicola, was applied in searching for natural agents against bacterial plant infections [18,29,32]. It is worth mentioning that the commercial Aliivibio fischeri test-Bioluminex™-is available on the market [33].…”

Section: Tlc-db (Thin-layer Chromatography-direct Bioautography)mentioning

confidence: 99%

“…The OPLC provides an efficient isolation of various substances from various matrices, including antimicrobial components, like essential oils of plants. Collected fractions can be concentrated and subjected to further experiments: TLC-DB, chemical derivatization and/or structural identification by spectroscopic methods [18,32,[56][57][58].…”

Section: Preparative Sample Isolationmentioning

confidence: 99%

See 1 more Smart Citation

TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants

Choma

Jesionek

2015

Chromatography

View full text Add to dashboard Cite

The richness of bioactive compounds in plant materials encourages continuous development of separation methods and bioassays for their isolation and identification. Thin-layer chromatography-direct bioautography links separation on the adsorbent layer with biological tests performed directly on it. Therefore, the method is very convenient for searching plant constituents with biological activity, such as antibiotics. Test bacteria grow directly on a plate surface excluding places where antibacterials are located. They can be detected with reagents converted by living bacteria. TLC-DB is a high throughput method enabling analyses of many samples in parallel and the comparison of their activity. Both screening and semi-quantitative analysis is possible. The targeted compounds can be identified using spectroscopic methods, mostly mass spectrometry, that can be performed directly on a TLC plate. This paper discusses all above mentioned aspects of TLC-DB, illustrating them with literature, schemes and our own results.

show abstract

Section: Tlc-db (Thin-layer Chromatography-direct Bioautography)mentioning

confidence: 99%

Section: Preparative Sample Isolationmentioning

confidence: 99%

TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants

Choma

Jesionek

2015

Chromatography

View full text Add to dashboard Cite

show abstract

“…So far, several methods for the isolation and identification of α‐bisabolol in chamomile and its products have been used . The purpose of the present work was to prepare a CNT/amino‐functionalized sol–gel coating on steel fiber suitable for SPME of α‐bisabolol from aqueous media.…”

Section: Introductionmentioning

confidence: 99%

A novel sorbent based on carbon nanotube/amino‐functionalized sol–gel for the headspace solid‐phase microextraction of α‐bisabolol from medicinal plant samples using experimental design

Yarazavi

Noroozian

2018

J of Separation Science

View full text Add to dashboard Cite

A novel sol-gel coating on a stainless-steel fiber was developed for the first time for the headspace solid-phase microextraction and determination of α-bisabolol with gas chromatography and flame ionization detection. The parameters influencing the efficiency of solid-phase microextraction process, such as extraction time and temperature, pH, and ionic strength, were optimized by the experimental design method. Under optimized conditions, the linear range was between 0.0027 and 100 μg/mL. The relative standard deviations determined at 0.01 and 1.0 μg/mL concentration levels (n = 3), respectively, were as follows: intraday relative standard deviations 3.4 and 3.3%; interday relative standard deviations 5.0 and 4.3%; and fiber-to-fiber relative standard deviations 6.0 and 3.5%. The relative recovery values were 90.3 and 101.4% at 0.01 and 1.0 μg/mL spiking levels, respectively. The proposed method was successfully applied to various real samples containing α-bisabolol.

show abstract

“…In other publications naturally luminescent marine bacterium, V. fischeri, and a luminescence gene-tagged plant pathogenic strain, Pseudomonas syringae pv. maculicola were used as bioautographic indicators [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Detection of antibacterial activity of essential oil components by TLC-bioautography using luminescent bacteria

Horváth

Kocsis

Lemberkovics

et al. 2013

Journal of Planar Chromatography – Modern TLC

Self Cite

View full text Add to dashboard Cite

Application of direct bioautography and SPME-GC-MS for the study of antibacterial chamomile ingredients

Cited by 15 publications

References 27 publications

TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants

TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants

A novel sorbent based on carbon nanotube/amino‐functionalized sol–gel for the headspace solid‐phase microextraction of α‐bisabolol from medicinal plant samples using experimental design

Detection of antibacterial activity of essential oil components by TLC-bioautography using luminescent bacteria

Contact Info

Product

Resources

About