Detection of antibacterial activity of essential oil components by TLC-bioautography using luminescent bacteria

Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds

“…The TLC plate is then coated with bioluminescent bacteria to reveal antimicrobial activity as dark zones on a luminescent background. The advantage of this kind of separation combined with post-chromatography bioassays is that it provides rapid and sensitive screening for individual antimicrobial compounds in various mixtures and can also be used for toxicity screening ( 57 , 58 ).…”

TLC-Direct Bioautography as a High Throughput Method for Detection of Antimicrobials in Plants

“…The developed TLC plate is immersed in a bacterial broth inoculated with luminescent bacteria and almost immediately observed at 490 nm under cooled CCD camera. The changes in emission of blue-green light (darker or brighter zones), related to disturbance in vital cell processes, indicate the presence of toxic substances [12,15,24,29,30] ( Figure 1). The image conversion and spreadsheet programs enable data interpretation [31].…”

“…Another luminescent test, using a genetically modified plant, pathogenic bacterium Pseudomonas syringae pv. maculicola, was applied in searching for natural agents against bacterial plant infections [18,29,32]. It is worth mentioning that the commercial Aliivibio fischeri test-Bioluminex™-is available on the market [33].…”

“…Antibacterial properties are not only attributed to essential oil components, like thymol, carvacrol or linalool, but also to more polar compounds seen below on the TLC plate. This simple example is only one from many others described in the literature proving that TLC-DB is an effective time-and money-saving tool for bioassay-guided detection of antibacterial components [18,24,29].…”

High-performance thin-layer chromatographic quantification of three active compounds from the decoction of Abnormal Savda Munziq prescription

“…The major advantage of HPTLC is that several samples can be run simultaneously using a small quantity of mobile phase unlike HPLC, thus, lowering analysis time and cost per analysis [27]. HPTLC has been widely used in the analysis of different pharmaceuticals, and it can be coupled with some new techniques and methods, such as thin-layer chromatography-mass spectrometry (TLC-MS) [28], TLC-diodearray detection (DAD) [29], and TLC-bioautography [30]. To this study, HPTLC is an ideal method which can be applied to quantitative analysis for ASMq because it is more rapid and practical than HPLC; the major components can be separated easily from this complex decoction, and multisamples can be analyzed on the same plate simultaneously.…”