High-performance thin-layer chromatographic quantification of three active compounds from the decoction of Abnormal Savda Munziq prescription

This method aims to analyze rosmarinic acid (RA), caffeic acid (CA), ursolic acid (UA) and oleanolic acid (OA) in different organs of Salvia deserta Schang qualitatively and quantitatively by high-performance thin-layer chromatography. Using chloroform–methanol–formic acid (10: 2: 0.5, v/v/v) as mobile phase and silica gel plate as stationary phase to analyze RA and CA at 330 nm. Using cyclohexane–ethyl acetate–methanol (10: 2: 0.5, v/v/v) as mobile phase and silica gel F254 plate as stationary phase to analyze UA and OA at 550 nm. The linearity ranges of RA, CA, UA and OA were 0.1250–0.4375, 0.0145–0.0870, 0.5000–2.5000 and 0.5000–2.5000 mg, respectively, with corresponding correlation coefficients of 0.9938, 0.9981, 0.9971 and 0.9969, respectively. Good precision, stability, accuracy (recovery rates were between 95 and 105%) of the method were determined. The limits of detection and the limits of quantification of RA, CA, UA and OA were determined, respectively, as 50, 58, 25, 33 and 160, 191, 80, 106 ng. The established method is simple, rapid, effective and can be easily used to determine the contents of RA, CA, UA and OA in different parts of S. deserta Schang.

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Qualitative and Quantitative Analyses of Four Active Components in Different Organs ofSalvia DesertaSchang by High-Performance Thin-Layer Chromatography

Wang

Tian

2022

Journal of Chromatographic Science

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Quantitative High-Performance Thin-Layer Chromatographic Analysis of Three Active Compounds in Gall ofQuercus infectoriaOlivier (Fagaceae) and Use of Thin-Layer Chromatography–2,2-Diphenyl-1-Picrylhydrazyl to Screen Antioxidant Component

Ou¹,

He²,

Ji³

et al. 2015

Journal of Planar Chromatography – Modern TLC

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Optimized extraction of artemisinin fromArtemisia annuaL. and corroborated quantitative analysis using high-performance thin-layer chromatography

Kundu¹,

Das²,

Ghosh³

2016

JPC - Journal of Planar Chromatography - Modern TLC

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High-performance thin-layer chromatographic quantification of three active compounds from the decoction of Abnormal Savda Munziq prescription

Cited by 5 publications

References 26 publications

Qualitative and Quantitative Analyses of Four Active Components in Different Organs ofSalvia DesertaSchang by High-Performance Thin-Layer Chromatography

Qualitative and Quantitative Analyses of Four Active Components in Different Organs ofSalvia DesertaSchang by High-Performance Thin-Layer Chromatography

Quantitative High-Performance Thin-Layer Chromatographic Analysis of Three Active Compounds in Gall ofQuercus infectoriaOlivier (Fagaceae) and Use of Thin-Layer Chromatography–2,2-Diphenyl-1-Picrylhydrazyl to Screen Antioxidant Component

Optimized extraction of artemisinin fromArtemisia annuaL. and corroborated quantitative analysis using high-performance thin-layer chromatography

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