Bioadhesive sulfacetamide sodium microspheres: Evaluation of their effectiveness in the treatment of bacterial keratitis caused by Staphylococcus aureus and Pseudomonas aeruginosa in a rabbit model

Şensoy, Demet; Cevher, Erdal; Sarıcı, Ahmet; Yılmaz, Mesut; Özdamar, Akif; Bergişadi, Nazan

doi:10.1016/j.ejpb.2009.02.006

Cited by 54 publications

(21 citation statements)

References 34 publications

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“…If left untreated bacterial keratitis often leads to progressive tissue destruction, with corneal perforation. Staphylococcus aureus, a common gram-positive bacterial pathogen implicated in bacterial keratitis, produces many biological active substances and toxins (Onlen et al, 2007;Sensoy et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Preparation and in vitro–in vivo evaluation of ofloxacin loaded ophthalmic nano structured lipid carriers modified with chitosan oligosaccharide lactate for the treatment of bacterial keratitis

Okur

Gökçe

Bozbıyık

et al. 2014

European Journal of Pharmaceutical Sciences

142

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Section: Introductionmentioning

confidence: 99%

Preparation and in vitro–in vivo evaluation of ofloxacin loaded ophthalmic nano structured lipid carriers modified with chitosan oligosaccharide lactate for the treatment of bacterial keratitis

Okur

Gökçe

Bozbıyık

et al. 2014

European Journal of Pharmaceutical Sciences

142

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“…It was surprising however, that of the tested three methods, 6 h incubation was the least proficient method. The literature shows that suspension of encapsulated products in a suitable dissolution medium is the most popular method (Blum and Saltzman 2008;Chandramouli et al 2004;Lyer, Kailasapathy and Peiris 2004;Ma et al 2008;Sensoy et al 2009;Thantsha et al 2009;Xiaoyan and Xiguang 2009;Yang et al 2008). The results indicate that, incubation of the encapsulated bacteria in simulated intestinal fluid for 6h will result in the underestimation of the viable bacteria present in the matrix, due to incomplete release.…”

Section: Resultsmentioning

confidence: 93%

Comparison of different methods for release of Bifidobacterium longum Bb46 from the poly(vinylpyrrolidone)-poly(vinylacetate-co-crotonic acid) interpolymer complex matrix, and the effect of grinding on the microparticles

Thantsha

Guest

Mputle

2011

World J Microbiol Biotechnol

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Bifidobacteria have been efficiently encapsulated in poly(vinylpyrrolidone)-poly(vinylacetate-co-crotonic acid) (PVP:PVAc-CA) interpolymer complex formed in scCO 2 . Research indicated that this method improves the stability of encapsulated bacteria in simulated gastrointestinal fluids in vitro. However, further analysis indicated release of lower numbers of encapsulated bacteria from the encapsulating matrix. The aims of this study were to determine a method that would release high numbers of bacteria from the PVP:PVAc-CA interpolymer complex matrix microparticles, and furthermore, to determine the effects of milling on the morphological properties of the microparticles. Three release methods, namely sonication, homogenization in a stomacher and incubation in simulated intestinal fluid (SIF) were compared. Released viable bacteria were assayed using plate counts. Viable bacteria released using a stomacher were three orders of magnitude higher than those released by incubation and an order of magnitude higher than those released using sonication. SEM indicated no negative effects such as exposure of encapsulated bacteria on the matrix due to milling of product. Homogenization in a stomacher is the most efficient method for releasing bacteria from the PVP:PVAc-CA interpolymer complex matrix. Particle size of the PVP:PVAc-CA microparticles encapsulating bacteria can be reduced further by grinding, without exposing the enclosed bacteria.

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“…Moreover, the presence of MC as a neutral polymer allows polymer swelling in water and helps entanglement of the polymer chains with the mucin molecules. All these contribute to the formation of a strengthened network (Andrews et al, 2009;Roy et al, 2009;Sensoy et al, 2009).…”

Section: Mucoadhesive Studiesmentioning

confidence: 97%

Moxifloxacin-gelrite In Situ ophthalmic gelling system against photodynamic therapy for treatment of bacterial corneal inflammation

et al. 2011

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In this study, six in situ gelling formulations based on Gelrite were prepared and evaluated for the retained ophthalmic delivery of Moxifloxacin (Mox). The effectiveness of the best developed formula G5 was compared with photodynamic therapy (PDT), the recent expanding approach for the treatment of ophthalmologic disorders after the assessment of optimum photodynamic inactivation parameters that permit efficient pathogens eradication. It was found that, Staphylococcus aureus (S. aureus) (Gram-positive) was more susceptible to effective lethal photosensitization that reaches 93.5% reduction in viable count than Escherichia coli (E. coli) (Gramnegative) of 76.1% using 3 mg/mL Hematoporphyrin (HP), illuminated by 630 nm Light Emitting Diode (LED) at 9 J/cm(2) and incubated for 15 min. Following topical instillation of G5 to rabbits corneas, higher amount of Mox was retained in the aqueous humor up to 24 h with significant 6-fold increase in the C(max) and AUC((0-∞)) compared to vigamox commercial eye drops. After post corneal infection with S. aureus, both approaches were effectively treating the infection without causing ocular irritation or collateral damage to corneal tissue where G5 showed remarkable improvement after four days compared to seven days of PDT treatment.

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Bioadhesive sulfacetamide sodium microspheres: Evaluation of their effectiveness in the treatment of bacterial keratitis caused by Staphylococcus aureus and Pseudomonas aeruginosa in a rabbit model

Cited by 54 publications

References 34 publications

Preparation and in vitro–in vivo evaluation of ofloxacin loaded ophthalmic nano structured lipid carriers modified with chitosan oligosaccharide lactate for the treatment of bacterial keratitis

Preparation and in vitro–in vivo evaluation of ofloxacin loaded ophthalmic nano structured lipid carriers modified with chitosan oligosaccharide lactate for the treatment of bacterial keratitis

Comparison of different methods for release of Bifidobacterium longum Bb46 from the poly(vinylpyrrolidone)-poly(vinylacetate-co-crotonic acid) interpolymer complex matrix, and the effect of grinding on the microparticles

Moxifloxacin-gelrite In Situ ophthalmic gelling system against photodynamic therapy for treatment of bacterial corneal inflammation

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